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L F Pomazanskaia

Publications and source records attributed to L F Pomazanskaia.

9 recordsLinked to original sources
PubMedDate not supplied

[Some peculiarities of brain phospholipids in deep sea fishes].

Total phospholipids (PL) as well as the content of various phospholipid classes and their fatty acid composition have been investigated in the brain of mesopelagic and abyssal marine teleosts. These species were compared to shallow water ones. The brain of deep sea fishes was found to be very poor in PL as compared to the brain of mesopelagic ans surface water species. No differences concerning the brain PL content were revealed between the two last mentioned groups. The relative content of separate PL classes was very similar in all the species studied irrespectively of the depth of their habitat. Peculiarities were found in fatty acid composition of individual PL from deep sea species as compared to surface ones. The deeper the habitat, the lower the content of saturated fatty acids, especially of the stearic acid. The lowest content of saturated fatty acids, maximum level of polyenoic fatty acids as well as some peculiarities in the relative content of particular fatty acids were found in the brain of ultraabyssal (6, 000 m) Leucicorus sp.

Adaptation, Physiological↗
PubMedDate not supplied

[Sodium reabsorption and the membrane systems of vertebrate kidneys].

Sodium reabsorption is low in coldblooded animals (the lamprey Lampetra fluviatilis, red salmon Oncorhynchus nerka, carp Cyprinus carpio, frog Rana temporaria, tortoise Agryonemis horsfieldi) being much higher in warmblooded ones (chicken, pigeon, rat). High level of Na transport in warmblooded animals is paralleled by high activity of succinate dehydrogenase as compared with the activity of Na,K-ATPase. The content of monoenoic fatty acids in the glycerophosphatides from frog and tortoise kidneys is higher than that from pigeon and rat ones.

Adenosine Triphosphatases↗
PubMedDate not supplied

[Comparative study of phospholipids in the neurons and glial cells of the brains of mammals, birds and reptiles].

Studies have been made on total content of phospholipids and relative content of various phospholipid families in neuronal and glial fractions obtained from the brain cortex of albino rat and brain hemispheres of the pigeon Columba livia and tortoise Testudo horsfieldi, as well as in oligodendroglial fraction of centrum semiovale and corpus callosum of the brain of rabbits. It was shown that in all the animals studied, the total glial fraction is approximately twice as rich in phospholipids as the neuronal one. With respect to the quantity of phospholipids, glial fraction is similar to the homogenate. Relative content of various phospholipid families in the homogenate and the cellular fractions is approximately identical. Yet, some differences were found between various fractions, as well as between the homologous fractions obtained from representatives of different classes. In all animals investigated, the neuronal fraction exhibits higher phosphatydylinositol content than the glial one. Pigeon neurons have higher level of phosphatydylinositol and lower level of phosphatydyl serine, as compared to rat and tortoise neurons. Glial fraction of rats contains significantly less sphingomyelin, than that of pigeons and tortoises. With respect to phospholipid composition, oligodendroglial fraction differs from total glial one and from neuronal fraction, being also different from the myelin.

Animals↗
PubMedDate not supplied

[Brain lipids of a mammoth, Elephas primigenius, which died more than 40,000 years ago].

Studies have been made on the brain lipids of the 6--7-month mammoth which remained in the eternal ice for more than 40.000 years. Thin layer chromatography of chloroformmethanol extract of the brain lipids shows that all glycerophospholipids in the brain were destroyed. On the contrary, sphingophospholipid sphingomyelin yielded the evident spot which was identified by specific reactions and by comparison with sphingomyelin from the brain of rat. Sphingomyelin content was evaluated. Using gas-liquid chromatography, fatty acid composition of sphingomyelin was investigated. It was found to be close to that in contemporary mammals. Other shingolipids -- cerebrosides, sulfatides, gangliosides -- persisted (probably, only partially) and were studied quantitatively. Relative content of cerebrosides with normal fatty acids and hydrooxyacids was determined. Studies were also made on fatty acid composition of cerebrosides, sulfatides and gangliosides, as well as on the composition of spingosine bases of gangliosides. Free cholesterol was found in the brain of the mammoth. Other sterols were not detected. With respect to quantitative evaluation of the preserved lipids, it should be mentioned that on the one hand, the brain underwent dehydration which increased lipid content per a unit of "wet" weight, whereas on the other one lipids were partially degraded, this process decreasing their content.

Animals↗
PubMedDate not supplied

[Method of extracting brain proteolipids].

From the whole brain of animals representing 5 classes of vertebrates--mammals (Macaca rhesus and albino rat), birds (the pigeon Columba livia), reptiles (the tortoise Testudo horsfieldi), amphibians (the frog Rana temporaria) and fishes (the trout Salmo irideus), as well as from myelin preparations of mammals (dog, rabbit and rat) proteolipids have been isolated by emulsion--centrifugation method. It was shown that extraction procedure results in the loss and destruction of several proteolipid complexes. In all the animals investigated, the main loss (from 25 to 75% of all complexes) was observed during emulsion--centrifugation stage, especially during 10-min centrifugation at 200 g. During extraction of proteolipids from the whole brain, under the conditions employed, the lost proteolipids are presumably those which belong to the myelin fraction. To prevent excessive proteolipid loss, the final low speed centrifugation step should be avoided. It was shown that in order to obtain proteolipids in a soluble form in a mixture of chloroform and methanol, total proteolipids from the brain of mammals should contain not less than 27% of phospholipids. Studies were also made on the composition and relative content of phospholipids in proteolipids extracted from the whole mammalian brain and myelin preparations. With respect to phospholipid composition, proteolipids from the whole brain significantly differ from those obtained from myelin fraction. Myelin fraction, being itself rich in proteolipids, contain large amounts of phosphatidyl serine (up to 69); however, proteolipids of this fraction contain lower amounts (4%) of diphosphatidyl glycerol as compared to proteolipids from the whole brain (22%).(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMedDate not supplied

[Brain proteolipids in representatives of different vertebrate classes].

The Folch-Lees proteolipid complexes of different purity (crude proteolipids and relative pure proteolipids) were isolated from vertebrate brain: mammalia (Macaca irus, Macaca rhesus and white rat), birds (Columbia livia), reptilia (Testudo horsfieldi), amphibia (Rana temporaria) and fishes (Salmo irideus). The proteolipid complexes were isolated by emulsion-centrifugation method. The content of proteolipid protein (mg/g w. w.) correlates with the level of phylogenetic development of the animals studied. It is the highest in monkey brain (10.5 and 8.6 mg/g) and the lowest in fish brain (2.2 mg/g). The yield of proteolipids from the brains of animals studied shows the same pattern. Crude proteolipids of mammalia, birds and reptiles contain 40-50% of protein and 60-50% of lipids. The content of phospholipids is about 40%. Proteolipids of amphibia and fish brain contain less protein--about 30%. In the conditions of mild purification, the protein content in mammalia, birds and reptilia makes up about 70% and lipid content--about 30-35%. The crude and purified proteolipids in all the animals studied (as compared with the original lipid extracts from which they were isolated) are enriched in acid phospholipids: phosphatidyl serine, phosphatidyl inositol and diphosphatidyl glycerol. Acid phospholipids in total lipid extract make up 10-20% of total phospholipids, in crude proteolipids 16-32 and in purified proteolipids--56-75%. There are no marked differences between fatty acid composition of phospholipids in proteolipids and in the same phospholipids isolated from total lipid extract.

Animals↗
PubMedDate not supplied

[Comparative study of the fatty acid makeup of the neuronal and glial cell phospholipids in the brain of reptiles, birds and mammals].

Studies have been made on fatty acid composition of phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol and sphingomyelin from the neurons and glial cells of the brain of rats, pigeons (Columba livia) and tortoises (Testudo horsfieldi). It was shown that fatty acid composition of the phospholipids investigated in both cellular fractions of tortoises differs from that of rats and pigeons. Within the same animal, fatty acid composition of homologous phospholipids from the neurons and glial cells is not identical. Differences in this respect were found in all the animals studied, although they are more evident in cells of pigeons and rats than in the cells of tortoises.

Animals↗