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L F Siqueira

Publications and source records attributed to L F Siqueira.

5 recordsLinked to original sources

[Stable solution of fuchsin--a new method for preparation of Ziehl-Neelsen staining].

The fuchsin salts currently available in Latin American market have shown some instability when in solution, according to the classic Ziehl-Neelsen method, resulting in a total precipitation of the salt. The authors indicate a new technique for the preparation of this solution, in order to minimize the action of interfering factors responsible for the precipitation, obtaining thus a greater solubility of the salt, as well as the solution stability. The method effectiveness is reinforced by the utilization of a smaller amount of the salt and the attainment of a larger storage period for the solution.

Chemical Precipitation

Rapid, automated identification of novobiocin-resistant, coagulase-negative staphylococci.

A modified automated method that uses the MS-2 system (Abbott Laboratories, Diagnostics Div., Irving, Tex.) to verify the reaction of coagulase-negative staphylococci to novobiocin is described. This technique permits the testing of a great number of specimens in an average time of 99 min and results in a 100% match with the traditional method of culturing.

Bacteriological Techniques