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L Füzesi

Publications and source records attributed to L Füzesi.

At least 19 recordsLinked to original sources
PubMed1998

Molecular assessment of p53 abnormalities at the invasive front of oral squamous cell carcinomas.

BACKGROUND: The prognostic significance of the invasive tumor front in squamous cell carcinomas has recently been recognized. The aim of the present study was to investigate possible molecular mechanisms underlying the significance of this area in oral squamous cell carcinomas. METHODS: We used immunohistochemical and molecular genetic techniques to investigate whether p53 alterations at the invasive tumor front could determine the aggressiveness of oral cancers. RESULTS: p53 Overexpression was detected in 52% to 56% (four different p53 antibodies) of 100 carcinomas studied. The concordance rate between results of immunohistochemistry and genetic analysis was 60%. No correlation was found between p53 status of the tumors and clinicopathologic parameters analyzed statistically. CONCLUSIONS: p53 Alterations have no prognostic impact in oral squamous cell carcinomas and apparently do not represent a molecular basis for the biologic significance of the invasive tumor front. The detection of discordant p53 aberrations between primary and second primary carcinomas in some patients provide evidence for their independent origin, with possible impact on prevention and therapy.

Adult↗
PubMed1998

Frequent loss of chromosome 12 in human epithelial ovarian tumors: a chromosomal in situ hybridization study.

The short arm isochromosome of chromosome 12 and trisomy 12 are well-established chromosomal alterations in human ovarian germ cell tumors. However, numerical aberrations of chromosome 12 in epithelial ovarian tumors (EOTs) are highly controversial; both trisomy 12 and monosomy 12 have been observed. We performed chromosomal in situ hybridization in paraffin-embedded and formalin-fixed tissue sections of 31 EOTs. Twenty-five EOTs could be evaluated statistically (2 mucinous, 11 serous, 5 endometrioid, 3 borderline, and 4 other epithelial-type tumors) to examine the copy number of chromosome 12 and 15. The frequency distribution of hybridization signals with alpha-satellite centromeric DNA probes for chromosome 15 revealed disomy in all cases. However, we found the loss of chromosome 12 in 16 of 25 tumor samples. No correlation was found between the presence of monosomy 12 and the clinical stage of the tumors. Frequent loss of chromosome 12 may indicate that this chromosome is involved in the tumorigenesis of EOTs. Further studies are needed to clarify whether loss of chromosome 12 is an early or late event in ovarian carcinogenesis.

Carcinoma, Endometrioid↗
PubMed1997

Detection of circulating prostatic cells during radical prostatectomy.

The detection of micrometastasis of prostate cancer could help to decide more appropriate therapeutic strategies in an individual patient. We have developed a flow cytometric method for detecting cytokeratin-positive cells in the peripheral blood before, during and after radical prostatectomy in patients with prostatic carcinoma. By means of this technique we were able to detect a higher number of cytokeratin-positive cells in the intraoperative blood sample than in the pre- and postoperative blood sample in 15 patients with prostate cancer (P < 0.05). Our results show an increase in the number of cytokeratin-positive cells with increasing tumor stage and grade, as well a good correlation of prostate-specific antigen (PSA) value with the number of cytokeratin-positive cells (r > 0.6). Our results underline the importance of no-touch techniques at prostatectomy to minimize release of tumor cells into the circulation during surgery. In the light of our results we consider that the indication for cell savers during radical prostatectomy should be reevaluated. The possibility of detecting single metastatic cells in peripheral blood will enable better individual patient management, and open up new modalities for diagnosing early prostate cancer and enhancing patient monitoring in relapse and tumor progression.

Aged↗
PubMed1997

Cytogenetic analysis of gliomas by in situ hybridization of stereotactic biopsy material.

Chromosome analysis of brain tumours can provide important pathobiological data; however, cytogenetic tools are so far not routinely applied for diagnosis. In the present study 25 paraffin embedded stereotactic biopsies from 19 glioma patients were studied using in situ hybridization of chromosome #10 and #15 using biotinylated pericentromeric probes. Numerical changes of chromosome #10 are frequent alterations in glioblastoma. Quantification of chromosome #15 served as a control in order to exclude artificial monosomies or nonspecific changes. The number of chromosomes in at least 200 cells were counted for each specimen. 18 of 25 biopsies could be evaluated quantitatively. The small volume of probes was not a limiting factor for analysis. Quantification of "nonspecific" chromosome #15 revealed single spots in 22-41% of all cells in the 18 biopsies. Chromosome #10 showed single spots in a range between 34 and 44% of counted nuclei in 13/18 biopsies. In 5 out of 18 biopsies 51-60% monosomies were found: in this subgroup were 4 high grade gliomas. These cases were interpreted as monosomy of chromosome #10. The results demonstrate feasibility and quantitative evaluability of cytogenetic analysis in stereotactic biopsy material using in situ hybridization.

Adolescent↗
PubMed1997

Computed tomography characterization of renal cell tumors in correlation with histopathology.

RATIONALE AND OBJECTIVES: The authors distinguish the histomorphologic subtypes of renal cell tumors (RCTs) by computed tomography (CT). METHODS: In a consensus conference between radiologists, pathologists, and urologists, the CT criteria of the various subtypes of RCTs (clear cell, chromophilic cell, chromophobic cell renal carcinoma and oncocytoma) were established. Computed tomography scans of 65 resected RCTs were reevaluated independently by seven radiologists. Using a numerical scoring system, they first attempted to differentiate clear cell from nonclear cell RCTs. A further attempt then was made to classify each tumor into one of the four categories. RESULTS: The sensitivity for the diagnosis of clear cell RCT was 72.5% (213 of 294 true-positive findings) and 82% (132 of 161 true-positive findings) for the nonclear cell group. For tumors more than 3 cm in diameter the sensitivities were 80.25% for the clear cell group and 80.7% for the nonclear cell group. Specific differentiation into the four subtypes was not possible. Oncocytomas were classified correctly in only 6 of 49 observations (12.2%). CONCLUSIONS: Small clear cell tumors often fail to show the CT characteristics that would permit an accurate classification. In tumors measuring 3 cm or more, differentiation between clear cell and nonclear cell types by means of CT criteria is possible. Nevertheless, as RCTs show a great variation in appearance, a differentiation into subtypes of the nonclear cell RCTs cannot be accomplished by CT. Using a uniform examination protocol and spiral scanning technique, the sensitivity of CT in the diagnosis of the subtypes of RCTs may be able to be further increased. Some tumors, especially oncocytomas, undoubtedly will remain diagnostic dilemmas.

Adenocarcinoma↗
PubMed1997

Novel enzyme immunoassay and optimized DNA extraction for the detection of polymerase-chain-reaction-amplified viral DNA from paraffin-embedded tissue.

Four different DNA extraction methods were compared to determine their ability to provide DNA for amplification of viral sequences from paraffin-embedded human tissue samples by polymerase chain reaction (PCR). The suitability of extraction methods was assessed using parameters like DNA yield, length of recovered DNA fragments, and duration. Furthermore, the efficiency of amplifying a human single-copy gene, the beta-globin gene, from DNA samples was tested. The best preservation of DNA molecules could be achieved by binding the DNA onto a silica column before further purification. Viral DNA sequences could be amplified by PCR in DNA extracted from routinely processed paraffin blocks from cases with clinically or morphologically suspected cytomegalovirus or Epstein-Barr virus infections. The PCR products were specified by a novel liquid hybridization assay called PCR-enzyme-linked immunosorbent assay. Using this assay, the time-consuming Southern hybridization could be replaced and the time requirement for the detection of PCR products could be reduced from 1 day to 4 hours. The assay system described here represents a reliable, sensitive, and specific method for the detection of viral DNA from paraffin-embedded tissue samples.

Cytomegalovirus↗
PubMed1997

Inflammatory pseudotumor of the epididymis.

We report the case of a 73-year-old patient who presented clinically with a palpable left scrotal mass after a 3-month history of therapy-resistant epidiymitis. He underwent epidiymectomy, and the following histopathologic and immunohistochemical evaluation revealed an inflammatory pseudotumor. We present the second case of an inflammatory pseudotumor of the epididymis being reported in the literature and give a brief review of the literature concerning this very rare neoplastic entity.

Aged↗
PubMed1996

Diagnosis of non-Hodgkin lymphoma of the maxilla with support of polymerase chain reaction.

Primary non-Hodgkin lymphoma of bone is an unusual extranodal presentation, and considerable difficulty exists in diagnosing some extranodal osseous lymphomas that develop in the head and neck region. Frequently, malignancies of the jawbones appear clinically as inflammatory diseases. Recognition of these cases usually occurs after the inflammatory-like symptoms have failed to respond to conventional therapy. This case report presents an extranodal non-Hodgkin lymphoma of the maxilla, which clinically imposed as odontogenic infection in a 45-year-old woman. The definite diagnosis was made by histologic means and confirmed by polymerase chain reaction (PCR). The latter is a molecular biologic method, which in unclear cases may be helpful in early differentiation of inflammatory from malignant processes.

Diagnosis, Differential↗
PubMed1996

Intratumorous heterogeneity of chromosome 10 and 17 in meningiomas using non-radioactive in situ hybridization.

Contrary to classical cytogenetics non-radioactive chromosomal in situ hybridization (CISH) may be performed within 24 hours while the morphological structure of paraffin-embedded tumor material is preserved. Slides of 34 formalin fixed and paraffin-embedded meningiomas were hybridized with a biotinylate alpha-satellite DNA-probe for chromosome 10 and 17. According to the distribution of hybridization signals per nucleus we found five meningiomas with signs of trisomy 17 whereby all of them exhibited intratumorous heterogeneity. Trisomy 10 was found in five tumors. Monosomy 17 was assumed in two cases and monosomy 10 in one meningioma. As a control six meningiomas were karyotyped by G-banding. Formalin fixed and paraffin-embedded tissue of the same tumors was hybridized against chromosome X and Y. In five of six cases the result of conventional karyotyping could be confirmed by in situ hybridization. CISH of paraffin-embedded tissue is new tool for the analysis of intratumorous cytogenetical heterogeneity with potential prognostic significance.

Adult↗
PubMed1996

Bellini duct carcinoma: a rare variant of renal cell carcinoma.

Bellini Duct Carcinoma (BDC) or collecting duct carcinoma is a rare but very aggressive renal neoplasm which originates from the epithelium of the ducts of Bellini in the distal tubule. This tumour often occurs in a young population and has a bad prognosis. Histomorphological differentiation from the more common renal cell carcinoma of the proximal tubuli is difficult. Immunohistochemic and cytogenetic characterisation can lead to the correct diagnosis.

Adult↗
PubMed1996

[Pancreatoblastoma--a rare pancreatic malignancy in childhood].

Between 1885 and 1991 only 71 cases of malignant pancreatic tumours in childhood and adolescence have been reported in literature; the majority of these were pancreatoblastomas. The symptoms, pathology and therapy of this rare tumour are demonstrated in the case of a 17-year-old girl. The tumour is believed to develop at an early stage of pancreatic cell differentiation. Usually it is composed of both exocrine and endocrine cell types. The treatment of choice is radical resection. In contrast to pancreatic neoplasms in adult patients the pancreatoblastoma has a favourable prognosis. The role of adjuvant chemotherapy or radiotherapy is still under discussion due to the small number of patients treated as yet.

Adolescent↗
PubMed1995

Endometrial stromal sarcoma with clonal chromosomal aberrations and mixed phenotype.

We report a case of a moderate-grade endometrial stromal sarcoma with the following chromosomal complement based on the evaluation of 43 metaphases: 47,XX,der(3)t(3;6)(q29;p21.1),der(6) t(3;6)(q21;q27), + 19. Immunohistochemically, the paraffin-embedded tumor tissue displayed positive vimentin reactivity and lack of cytokeratin expression, indicating a mesenchymal origin. Interestingly, the cultivated tumor cells revealed a co-expression of vimentin and different subtypes of cytokeratin. Therefore, the cytogenetically monoclonal tumor cells which showed co-expression of epithelial and mesenchymal phenotypes suggest that the endometrial stromal sarcoma can be interpreted as a monophasic variant of the malignant mixed Müllerian tumor.

Aged↗
PubMed1995

[The percutaneous CT-guided treatment of osteoid osteomas: a combined procedure with a biopsy drill and subsequent ethanol injection].

PURPOSE: The purpose of this study was the clinical evaluation of a percutaneous treatment modality in patients with an osteoidosteoma. PATIENTS AND METHODS: 6 patients with an osteoidosteoma of the upper (n = 1) and lower (n = 5) extremity which were confirmed on plain film radiographs and computed tomography underwent CT controlled drill biopsy of the nidus with subsequent injection of 96% ethanol into the biopsy channel to sclerose probably remaining remnants of the nidus. The procedure was started under local anaesthesia, but drilling of the nidus was carried out under a short general anaesthesia using ketamine. RESULTS: The intervention was successful in all patients. No postinterventional infection occurred and no recurrence was observed in any of the patients in a follow-up time between 0.5 and 2 years. CONCLUSION: Although only 6 patients were treated until now, we conclude that this combined procedure using a bone biopsy system and the subsequent injection of alcohol is a safe and successful procedure for percutaneous treatment of osteoidosteoma.

Adolescent↗
PubMed1995

[Sterility as a sequela of tubal schistosomiasis].

A 21-year old african women presented with primary sterility in the outpatient department of our university hospital. Screening examinations, including ultrasound, demonstrated a tumour of 5.8 x 4.1 cm diameter on the left ovary. While performing laparotomy to extirpate this tumour, bilateral occlusion of the tubes was discovered by chromopertubation.This diagnosis led us to perform bilateral salpingostomy and reimplantation of the Fallopian tubes. In addition to the ovarian tumour (dermoid), fragments of the Fallopian tubes were sent for histological examination, which revealed eggs of schistosomes probably of the species Schistosoma haematobium. Since tourism to and immigration from endemic areas is increasing, schistosomiasis should be considered when it is not possible to elucidate the cause of sterility by classical means.

Adult↗
PubMed1994

Morphometric study of centrilobular vessels in the rat liver after continuous hypothermic perfusion with Euro-Collins and University of Wisconsin solution.

Damage to the hepatic microcirculation plays an important part in the preservation-related loss of graft viability. In a study on the isolated, hypothermically preserved rat liver perfused continuously with Euro-Collins and University of Wisconsin solution (gluconate substituted for lactobionate) at different flow rates we determined changes in diameter of central veins and pericentral sinusoids and related them to weight changes. While post-perfusion liver weight increased with Euro-Collins and decreased with University of Wisconsin solution, weight changes did not vary significantly with different flow rates. Changes in sinusoidal diameter depended on the type of solution as well as on the flow rate. Cell swelling under Euro-Collins perfusion leads to compression of pericentral sinusoids, which varies in degree with different flow rates. Perfusion with University of Wisconsin solution under near-normal flow, due to slight shrinking of parenchymal cells, keeps the sinusoidal lumina wide open. We conclude that there is a causal relationship between perfusion-dependent cell swelling and reduction in sectional area of the hepatic microvasculature. Continuous hypothermic perfusion with University of Wisconsin solution at physiologic flow rates excellently preserves the size and trabecular architecture of hepatocytes and thereby the sinusoidal lumina within the hepatic lobules.

Adenosine↗