Biomedical subjects

L Fabry

Publications and source records attributed to L Fabry.

40 records · Page 3Linked to original sources

PubMed1978

Mutagenicity tests with styrene oxide in mammals.

The capability of styrene oxide to induce chromosome damage in vivo has been tested in the male mouse by examination of bone-marrow cells, by scoring micronuclei in polychromatic erythrocytes, by observation of meiotic chromosomes from treated males and by the dominant-lethality test. Furthermore, studies have been performed on short-term cultures of human lymphocytes in vitro. Whereas an increase in the yield of chromatid and chromosomal aberrations was observed after exposure in vitro, only negative results were obtained in the tests in vivo. One has, therefore, to conclude that styrene oxide is potentially capable of breaking mammalian chromosomes but that an exposure to an acute dose in vivo does not produce visible damage in somatic cells or in male germ cells.

Animals↗

PubMedDate not supplied

Effect of low-dose acute X-irradiation on the frequencies of chromosomal aberrations in human peripheral lymphocytes in vitro.

In a coordinated research programme sponsored by the International Atomic Energy Agency, the frequencies of chromosomal aberrations induced in peripheral blood lymphocytes (in vitro) by 250 kV X-rays at low doses (0.4, 1, 2, 3, 5, 10 and 30 rad) were determined. Blood from 2 donors was used to conduct one master experiment at these dose levels. The culture time used was 48 h and all samples including the controls were processed according to a standard protocol. The coded slides were scored by investigators from 10 participating laboratories. The main results are the following: (1) the frequencies of all types of chromosome aberrations at 0.4 rad are significantly lower than the control values; (2) there is no increase in the frequencies of dicentrics up to 2 rad and in those of terminal deletions up to 5 rad; (3) the mean frequencies of all aberrations considered together are not significantly different from one another at 1, 2 and 3 rad (P = 0.05); and (4) over the entire dose range the dose-effect relationship is clearly non-linear. A fit of these data to a linear quadratic model (E(D) = c + alpha D + beta D2) showed that the observed total aberration frequencies at doses 1, 2, 3 and 5 rad are below the curve defined by the model. The deviations can be explained by an altered kinetics of aberration production at very low doses probably due to DNA repair mechanisms operating these cells.

Adult↗

PubMedDate not supplied

Lack of mutagenicity of diphenylhydantoin in in vitro short-term tests.

The mutagenicity of diphenylhydantoin (DPH) and its major metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin (HPPH), has been re-evaluated by the Ames test using Salmonella typhimurium and, for DPH only, by an in vitro cytogenetic test with human lymphocytes and a turbidimetric assay of tubulin polymerization. As negative results were obtained in all test systems used here, one has to conclude that DPH is devoid of mutagenic properties.

Aflatoxin B1↗

PubMedDate not supplied

Cytogenetic damage induced in human lymphocytes by low doses of 60Co gamma rays delivered at high and low dose rates.

Chromosomal aberrations were investigated in human peripheral blood lymphocytes after exposure to low doses of 60Co gamma rays delivered acutely or at low dose rates (0.1 or 0.03 Gy/h). Chromosome analysis was performed in cells collected after a 44 to 46 hour culture time in order to avoid scoring of second division cells, and the dose-related induction of aberrations was analysed by the maximum likelihood method. In all cases, the induction of dicentrics was well described by a linear-quadratic dose response model (Y = aD + bD2), the data obtained at a low dose rate being equally well fitted to a linear equation. According to earlier findings on the mechanisms of aberration formation, the two lesions originating from single ionizing tracks have to be produced within a period of approximately 5 hours, in order to interact and to give rise to dicentric aberrations, which could explain the decrease in the quadratic term at the low dose rate since the highest doses were delivered over a period of more than 5 hours.

Cell Division↗