PubMed Health⌕ Search

Biomedical subjects

L Fernando

Publications and source records attributed to L Fernando.

12 recordsLinked to original sources
PubMed1989

Effects of in utero exposure to cyclophosphamide in mice. II. Assessment of immunocompetence of offspring from 5 to 10 weeks of age.

Offspring of mice treated with cyclophosphamide (Cy; 1, 2.5 or 5 mg/kg) during pregnancy (6-18 days of gestation) and tested for immunocompetence from 5 to 10 weeks of age were found to have defective reticuloendothelial clearance. The main effects were: a) increased elimination half time (T 1/2) of 51Cr-labeled SRBC from circulation, b) decreased liver uptake of 51Cr and c) impaired ability of the spleen, mostly affecting the female pups, to compensate for decreased liver uptake. The highest dose group suffered the most pronounced effects. This group was also found to have increased IgG immunoglobulin levels at 7 weeks of age. IgG antibody production in response to specific antigenic stimulation and delayed hypersensitivity reactions to oxazolone did not appear to be affected by Cy treatment.

Aging↗
PubMed1988

An asparaginase of Aspergillus nidulans is subject to oxygen repression in addition to nitrogen metabolite repression.

Of five amidohydrolase activities subject to nitrogen metabolite repression in Aspergillus nidulans, L-asparaginase shows clearest evidence of also being subject to repression by atmospheric oxygen. Such oxygen repressibility is only evident under nitrogen metabolite derepressed conditions. Asparaginase levels are also considerably elevated by areA300, an altered function allele of the positive acting wide domain regulatory gene areA mediating nitrogen metabolite repression and are drastically reduced by loss of function mutations in areA. A. nidulans has two L-asparaginase enzymes and it has been shown by the use of appropriate mutants that these regulatory effects are exerted on the expression of that specified by the ahrA gene but probably not that specified by the apnA gene.

Aerobiosis↗
PubMed1986

Early detection and treatment of spinal epidural metastases: the role of myelography.

Spinal epidural metastases were detected in 75 of 140 cancer patients with back pain who were evaluated prospectively by clinical criteria, spine roentgenography, and bone scan. Fifty-five of the 75 patients with epidural metastases had no evidence of myelopathy when diagnosed. Of the patients diagnosed and treated while still ambulatory, more than 90% remained so. Myelograms were performed in 127 patients to diagnose the 75 with epidural disease. To try to reduce the number of myelograms needed, we attempted to design radiotherapy ports based on clinical symptoms and the plain spine films alone. A port could not be designed for 64 of the 127 patients, either because of diffuse vertebral metastases or a normal plain roentgenogram. A port could be designed for 63 patients, and all epidural disease would have been encompassed in 50 of the 54 patients who had spinal epidural metastases (93%). Most patients with cancer and back pain require myelography for accurate treatment planning. There are, however, situations in which treatment can be determined based on symptoms and plain films alone, with a low risk of missing epidural cancer.

Back Pain↗
PubMedDate not supplied

Identification of HIV-infected transfusion recipients: the utility of crossreferencing previous donor records with AIDS case reports.

An expanded "look-back" program has been developed, in which patients who may have been exposed to HIV through blood transfusion were identified via three triggers: the blood that they received was donated by persons who 1) have subsequently been reported to local health departments as meeting the diagnostic criteria for AIDS, 2) have donated since the introduction of anti-HIV screening and tested positive, or 3) have been found to be infected during investigation of reported transfusion-associated HIV infections. In comparing triggers, cross-referencing the list of patients reported to have AIDS (AIDS case list) proved to be the most efficient approach for identifying HIV-infected donors and transfusion recipients. Of the 7973 AIDS patients reported in eight Northern California counties as of December 1988, 316 (4.0%) were determined to have donated at Irwin Memorial Blood Centers between 1977 and 1985. Despite the logarithmic increase in reported cases of AIDS in the San Francisco Bay area over the last 5 years, the rate of detection of previous donors from AIDS case lists remained between 3.3 and 5.4 percent. These persons accounted for 69 percent of the identified infected donors, and their identification led to notification of 60 percent of potentially exposed recipients and 51 percent of known infected recipients. The crossreferencing of AIDS case listings with blood bank records was equally efficient for regions with low and high AIDS prevalence. National adoption of AIDS case list crossreferencing is recommended as an effective means of identifying previously unidentified infected recipients in an effort to limit the secondary spread of HIV infection.

Acquired Immunodeficiency Syndrome↗