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Biomedical subjects

L Ferrer

Publications and source records attributed to L Ferrer.

At least 91 records · Page 5Linked to original sources
PubMed1990

Immunocytochemical demonstration of intermediate filament proteins, S-100 protein and CEA in apocrine sweat glands and apocrine gland derived lesions of the dog.

The presence of carcinoembryonic antigen (CEA), intermediate filament proteins and S-100 protein in normal and pathological canine apocrine sweat glands was investigated, using a standard immunoperoxidase technique. The normal apocrine sweat glands showed a constant immunoreactivity in all the cases studied. The cells of the acini and of the ducts only reacted with the antikeratin antibody. The myoepithelial cells reacted positively with the antisera antikeratin and anti protein S-100. Epithelial cells of apocrine cysts, sweat gland adenomas and sweat gland carcinomas showed the same immunoreaction than normal apocrine cells. Proliferating myoepithelial cells were also positive for vimentin. In two out of three adenocarcinomas a positive reaction with the anti CEA could be detected in the glandular cells. This can be due to the presence in glandular cells of CEA or of Nonspecific Crossreacting Antigen (NCA). These findings indicate that demonstration of keratin is a useful aid in the detection of apocrine gland derived lesions in the dog. Similarly, S-100 protein is a marker for myoepithelial cells. Further research is necessary to investigate the expression of CEA in canine tumours.

Animals↗
PubMed1989

Immunohistochemical localization of S-100 protein and lysozyme in canine lymph nodes and lymphomas.

The present study concerns the immunocytochemical localization of S-100 protein and lysozyme in the cells of canine lymph nodes and lymphomas. In the normal canine lymph node S-100 protein was detected in follicular dendritic cells (FDCs) and in interdigitating reticulum cells (IRCs). S-100 positive cells were found in thirteen out of twenty-four lymphomas, scattered throughout the tumour or in follicles. In three cases (two cutaneous lymphomas and one nodal lymphoma) the number of positive cells was high, although the main proliferating cell population was S-100 negative. In normal lymph nodes lysozyme immunoreactivity was found in the cytoplasm of cortical and medullary histiocytes. Lymphomatous cells were negative for lysozyme, although in three cases lysozyme-positive cells were present between the lymphatic cells. The possible origin of these S-100 or lysozyme positive cells is discussed. It is suggested that S-100 protein and lysozyme can be used as useful markers for these two types of dendritic cells and for macrophages.

Animals↗
PubMed1989

Immunocytochemical diagnosis of skin tumours of the dog with special reference to undifferentiated types.

Immunoperoxidase techniques for S-100 protein, keratin, cytokeratin, vimentin and desmin were applied to 65 canine skin tumours. These included eight squamous cell carcinomas, eight fibrosarcomas, eight melanomas, eight mastocytomas, eight haemangiosarcomas, eight leiomyosarcomas, five liposarcomas and 12 poorly differentiated tumours. Consistent results were obtained within each group. Cytokeratin and keratin immunoreactivity was detected only in squamous cell carcinomas. Vimentin was present in fibrosarcomas, melanomas, haemangiosarcomas, mastocytomas, leiomyosarcomas and liposarcomas. S-100 protein immunoreactivity was detected in melanomas, haemangiosarcomas, liposarcomas and leiomyosarcomas. Only leiomyosarcomas were positive for desmin. According to these results the 12 anaplastic tumours were diagnosed either as carcinomas, fibrosarcomas or malignant melanomas.

Animals↗
PubMed1989

Alpha-thalassemia changes the cell density profile in sickle cell anaemia.

Several factors are implied in the haematological and clinical picture of sickle cell anaemia. Attention has been focused on the concomitant presence of -alpha-thalassemia and high levels of HbF, but contradictory results have been reported in different populations. We compared the blood cell density profile, obtained by the phtalate esther method, of normal subjects with those of patients with sickle cell anaemia - with or without heterozygous alpha-thalassemia. We found that the density profile of both groups of patients differs from normal subjects, and that a difference can also be demonstrated between normal alpha genotype patients with sickle cell anaemia and patients with heterozygous alpha-thalassemia. These results are in agreement with the findings obtained in other countries in which a gene from Caucasian to African populations have been demonstrated, and are different from the results obtained in populations of more pure African ancestry. It can be suggested, therefore, that these data, in addition with findings of other authors in different geographical areas, support the hypothesis that the genetic make up plays an important role in the haematologic and clinical picture of sickle cell anaemia.

Anemia, Sickle Cell↗
PubMed1988

Identification of Leishmania donovani amastigotes in canine tissues by immunoperoxidase staining.

This paper describes the demonstration of Leishmania donovani amastigotes in canine tissues by immunoperoxidase staining. An indirect immunoperoxidase method was applied to the organs of 20 dogs in which leishmaniasis was previously diagnosed. Haemosiderin pigment was eliminated with 5 per cent oxalic acid. Amastigotes of L donovani appeared as dark brown stained bodies which contrasted with haematoxylin stained host cells. No positively stained amastigotes could be seen in any of the sections incubated with control serum. The organs which more frequently showed leishmanids were: skin (macrophages and fibroblasts), liver, spleen, lymph nodes and bone marrow. In a few cases amastigotes were seen in kidneys, gut, adrenal glands, eyes and testicles. This technique is simple to perform, gives consistent results and allows unequivocal histopathological diagnosis of canine leishmaniasis.

Animals↗
PubMedDate not supplied

Electron microscopy in pathology articles: a retrospective appraisal.

Electron microscopy (EM) is a valuable standard tool in basic research and teaching. However, its use in diagnosis is limited, either for strategic reasons or budgetary constraints. This means that its many potential applications are more often neglected, either as an ancillary tool, quality control method, or gold standard, to complement, support, or confirm results of pathological studies. To evaluate the use of EM in this setting, the authors analyzed all articles (n = 2,531) in the three top indexed diagnostic pathology journals for a period of 60 months from July 1993 to June 1998. A total of 448 articles in which the use of EM was indicated, according to standard surgical pathology textbooks, were selected. Both the actual and the potential EM content of each article were scored, as follows: zero, illustrative, supportive, gold standard (for confirmation of research results), extensive, and predominant. Of the total number of articles in which EM was indicated, 77% made use of the technique. EM support was lacking most frequently in articles on serosal neoplasms and on new diagnostic strategies (p < .00005). There was no definite trend toward an increase or decrease in the use of EM during the period analyzed. The authors conclude that EM is used in most reports on diagnostic pathology, when it is indicated. However, a small but non-negligible percentage of articles (23%) could benefit from including EM as an ancillary, control, or gold standard method to complement, support, or confirm their results.

Diagnosis, Differential↗
PubMedDate not supplied

Cryptococcosis in a cat seropositive for feline immunodeficiency virus.

A 5-year-old female Siamese cat was presented to the veterinary teaching hospital with a history of bronchopneumonia for 20 days. The cat had not responded to antibacterial chemotherapy and had developed a pronounced submandibular lymphadenopathy. Characteristically encapsulated yeast cells with narrow-necked buds were clearly seen in a fine-needle aspirate of the lymph node with an India ink preparation. Cryptococcus neoformans var. neoformans was identified. Susceptibility tests on the isolated strain were performed using antifungal tablets. The strain was sensitive to amphotericin B, fluconazole, itraconazole and ketoconazole and was resistant to 5-fluorocytosine. The cat was positive for feline immunodeficiency virus. Nevertheless, the cat was treated with ketoconazole for 3 months and apparently recovered. Three months later the animal was presented in a precomatose state. The owners refused to treat the animal and the cat was destroyed.

Animals↗
PubMedDate not supplied

Canine mucosal leishmaniasis.

Four dogs infected with Leishmania had proliferative lesions on the mucosae of the penis, tongue, oral cavity, prepuce, or nose. These mucosal, nodular lesions produced by parasites of the genus Leishmania have not been described previously in the dog. Leishmaniasis should be considered in the differential diagnosis of tumor-like lesions of mucous membranes.

Animals↗
PubMedDate not supplied

[Non-aneurysmatic aortic dysphagia].

Esophageal compression by a vascular structure is a rare cause of dysphagia, the aberrant right subclavian artery being the most common congenital abnormality. Aortica dysphagia is usually observed in the elderly, especially in hypertensive women with cardiopathy and degenerative osteopathy. We report a 73-year-old woman with dysphagia, caused by a non-aneurysmatic aortic elongation, who presented progressive dysphagia, which ended in aphagia associated with heart failure. The diagnostic approach to these patients is discussed. The patient received cinitapride and, following treatment for heart failure, remains asymptomatic after a 3-year follow-up period, although manometric alterations persist.

Aged↗
PubMedDate not supplied

Relationship between gastric levels and antiulcerogenic activity of zinc.

The relationship between the absorption of an organic zinc salt, zinc acexamate, and its antiulcerogenic activity in a model of cold-restraint stress was studied. Serum and gastric levels of zinc, as well as its antiulcerogenic effect, were determined after oral or intravenous administration of zinc acexamate. Cytochemical and X-ray microanalysis techniques were also applied. In the rats subjected to cold-restraint stress, gastric levels of zinc correlated with the antiulcerogenic effect observed after administration of zinc acexamate. However, it was not possible to establish a relationship between serum levels and the pharmacologic effect of zinc. Our results in animals subjected to regular diet indicate that the antiulcerogenic effect exhibited by zinc compounds could be associated with the presence of zinc at different levels of gastric tissue.

Administration, Oral↗