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L Ferreras

Publications and source records attributed to L Ferreras.

2 recordsLinked to original sources

Soil bacterial functional diversity as influenced by organic amendment application.

The aim of the present work was to assess the response of microbial functional diversity to organic soil amendment and the relationship between the microbial functional diversity and soil carbon availability. Household solid waste compost, horse and rabbit manure and chicken manure were applied at two doses. The same undisturbed soil type (Vertic Argiudoll) was used as reference. Soil suspensions were applied to Biolog EcoPlate and average color development (AWCD), richness (R) and Shannon-Weaver index (H) were calculated. The amendment incorporation resulted in significant increases (p < 0.05) in AWCD, R and H compared to the unamended plots. The regression functions showed linear relationships when R and H were related to soil organic carbon (R(2) = 0.77 and 0.72, respectively). Principal component analysis allowed the differentiation of treatments from the control and the undisturbed sites. The Biolog EcoPlate assay was sensitive to changes in the short term due to management practices.

Agriculture↗

Early decrease in GLUT4 protein levels in brown adipose tissue of New Zealand obese mice.

The aim of this study was to determine if the previously described insulin resistance in the New Zealand Obese (NZO) mouse is associated with a decrease in GLUT4 protein and if such changes occur early in the evolution of the syndrome. GLUT4 levels were measured in whole membranes isolated from a variety of tissues in 4 and 20-week-old NZO and control NZC mice by Western blotting using a specific antibody to the C terminal end of the protein. At 20 weeks of age, GLUT4 levels were lower in the NZO mice in brown and white adipose tissue, heart, diaphragm, red and white quadriceps, and red and white gastrocnemius, but not in soleus muscle. At 4 weeks of age, GLUT4 levels were 52% lower in BAT (3309 +/- 1006 vs 6951 +/- 1870 cpm P = 0.039) but were not lower in WAT, heart or red quadriceps. It is concluded that adult NZO mice have a decrease in GLUT4 levels in most insulin-sensitive tissues and that in BAT, this occurs at an early age.

Adipose Tissue↗