[A new approach-pancreatoduodenectomy with preservation of the pylorus--in the surgical treatment of diseases of the pancreas head].
Explore the source record for details and available documents.
Adult↗
Biomedical subjects
L Flautner
Publications and source records attributed to L Flautner.
Changes in blood coagulation due to pancreas transplantation in dogs.
Blood coagulation was studied in the course of 11 pancreatectomies and 7 pancreas transplantations. Comparison of the postoperative changes in the fibrinogen level suggests the important role of the normal pancreas in fibrinogen generation. Investigation of the indices of the coagulation and fibrinolytic system revealed in the post-transplantation period a shift in the activity of the coagulation indices towards hypercoagulability. The increased fibrinogen level, the inhibited fibrinolysis and the elevated activity of the coagulation factors are assumed to be responsible for the high incidence of thrombotic complications associated with transplantation of the pancreas.
Animals↗
[Enzyme-histochemical studies of ischemic damage to the pancreaticoduodenal graft ready for transplantation in the dog].
The ischaemic lesion of the pancreaticoduodenal graft prepared for transplantation was studied by enzyme histochemical methods. Estimation of lactate dehydrogenase, non-specific acid and alkaline phosphatases and esterases and of glucose-6-phosphatase pancreas. While in the pancreas, lesions due to warm ischaemia will appear after one hour, the same can be observed in the duodenum after 30 minutes and in both organs the lesions progress rapidly. Lesions resulting from cold effects appear in the pancreas after three hours and remain unchanged for 15 to 18 hours, while in the duodenum they will appear after one hour and deteriorate considerably after three hours. Continuous perfusion for more than 30 minutes or the glucose perfusion test performed after cold ischaemia of more than two hours duration considerably affected the enzymes of the organs.
Acid Phosphatase↗
[Surgically treated mucocele of the vermiform process].
Explore the source record for details and available documents.
Appendectomy↗
Inhibitory effect of a long-acting somatostatin analogue on EGF-stimulated cell proliferation in Capan-2 cells.
Numerous studies have reported diverse effects of gut-derived regulatory peptides on growth of the normal pancreas, pancreatic neoplasms induced experimentally in animals, and pancreatic cancer cell lines, but the results of these investigations are rather controversial. The stimulatory effect of epidermal growth factor (EGF) on cell proliferation of pancreatic cell lines is well established. Whether this action can be modulated by somatostatin is not clear. Furthermore, it is not certain whether another regulatory peptide, cholecystokinin (CCK), affects the proliferation of these cells. In the present study we investigated the presence of CCK-A and CCK-B, as well as somatostatin-2 (SSTR2) receptors by RT-PCR, and studied the actions of EGF, CCK and octreotide on DNA synthesis in the human pancreatic adenocarcinoma cell line Capan-2. Octreotide, a long-acting somatostatin analogue was used as somatostatin agonist. Cells were cultured in RPMI-1640 medium. They were incubated in serum free medium containing 0.2% BSA in the absence (control) or the presence of the peptides. [3H]-thymidine incorporation into DNA was measured after 48 h of incubation. By means of RT-PCR analysis we were able to demonstrate SSTR2 expression, but not CCK-A or CCK-B receptor mRNA in Capan-2 cells. DNA synthesis evaluated by [3H]-thymidine incorporation was found to be increased by 45.2 +/- 5.6% in response to EGF (10(-8) M) and decreased by 11.7 +/- 2.6% to octreotide (10(-8) M) compared to controls (P < 0.01). The increase in [3H]-thymidine incorporation was significantly lower when EGF treatment was combined with octreotide administration (10.1 +/- 2.5% over control). In the concentration range of 10(-11)-10(-8) M, CCK did not alter significantly the incorporation of [3H]-thymidine into DNA in Capan-2 cells. In conclusion, these data support a role for EGF as a growth factor for the human pancreatic cancer cell Capan-2. Somatostatin may play an important role in regulating cell proliferation in Capan-2 cells both under basal, and growth factor-stimulated conditions. Our results suggest, however, that CCK receptors are not expressed, and CCK does not affect cell proliferation in this transformed pancreatic cell line.
Adenocarcinoma↗
Ultrastructural study of a black insulinoma.
A case of black insulinoma is reported. The color was due to a cytoplasmic pigment. Immunostaining for neuron-specific enolase and chromogranin was positive in the tumor cells, and the pigment granules themselves reacted with the chromogranin antibody. Numerous beta cell type dense core granules as well as atypical granules were found by electron microscopy. An important finding was that the dense core granules contribute to the lipofuscin pigment formation.
Aged↗
The outcome of pancreatic fistulas developed after surgery for pancreatic pseudocysts.
Pancreatic fistulas (PF) develop in about 20% of cases operated for pancreatic pseudocysts. The authors analyse 81 cases of postoperative PF. These were formed following 991 operations performed for pancreatic pseudocysts (PPCs) in 850 patients from 1987 to 1992. It is concluded that the incidence of PF formation is significantly (P < 0.01) increased following interventions for acute-type pseudocysts and after external drainage. One-third of the fistulas closed spontaneously within 1-4 weeks, while another 1/3 persisted for 1-6 months before gradual closure. Closure of fistulas was facilitated by inhibition of pancreatic secretion with Somatostatin or endoscopic intervention (EST, endoprosthesis) in 24% of all cases. Only 15% (14 cases), of the fistulas, i.e. 1% of total patients, required surgery. The procedure of choice in the 14 cases was exstirpation of fistulas alone (2 cases) or combined with necrectomy (10 cases), or with distal pancreatic resection (2 cases). In cases of drained pancreatic fistulas observation can be an appropriate treatment option, while long-standing fistulas producing large amounts require intervention.
Adult↗
Occurrence and significance of Helicobacter pylori infection after gastric surgery.
Occurrence and significance of Helicobacter pylori (Hp) infection in patients having undergone partial and total gastric resection was studied. Forty-nine patients after gastric resection with Billroth I and II operation (28 patients due to ulcer and 21 patients due to gastric cancer), and 8 patients with total gastrectomy due to gastric cancer were selected. In addition, 40 patients with active peptic ulcer disease, 32 patients suffering from gastric cancer, and 28 patients with non-ulcer dyspepsia as a control group were enrolled. Occurrence of Hp in patients having been operated on due to ulcer disease was slightly lower than in controls (50% vs. 54%), but it was significantly lower than in patients with active peptic ulcer (50% vs. 85%, p < 0.001). On the other hand, in patients who were operated on due to gastric cancer, Hp was much less frequent than in the control group, as well as in patients in the preoperative period (9.5% vs. 54% and 72%, p < 0.001). As a conclusion, since in our series colonization with Hp was found to be less frequent after gastric resection than before surgery, its aetiologic significance in postoperative chronic gastritis and ulceration seems to be unlikely. Therefore, the routine postoperative eradication of Hp appears to be unnecessary in such patients.
Adult↗