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L Fornůsek

Publications and source records attributed to L Fornůsek.

31 records · Page 2Linked to original sources
PubMed1984

Comparison of some properties of normal or stimulated murine peritoneal macrophages.

We studied phagocytic activity of murine macrophages for 2-hydroxyethylmethacrylate particles, determined the percentage of cells carrying Fc receptors for IgG1, IgG2A, IgG2B, IgM, IgA and IgE, and phagocytic activity mediated by Fc receptors, as well as pinocytic activity and adherence of normal and thioglycollate- or proteose peptone-stimulated peritoneal macrophages. A significant increase in phagocytic activity was found (the exceptions were FcR for IgG2A, IgG2B and IgM in TG-stimulated and FcR for IgA in PP-stimulated macrophages). Pinocytic activity increased two- to three-fold, adherence capacity, on the contrary, fell to one third in the TG-stimulated macrophages, whereas it remained unchanged after treatment in the PP-stimulated ones. Further evidence is provided for considerable differences in the properties of normal and stimulated peritoneal macrophages.

Animals↗
PubMed1983

An advantageous method for detection of Fc-receptors and for studying Fc-receptor-mediated phagocytosis.

A method employing fluorescent 2-hydroxyethyl methacrylate copolymer particles with dinitrophenyl haptenic group and anti-haptenic murine monoclonal antibodies of different isotypes is described for tracing Fc-receptors and for studies of phagocytosis via these cell surface structures. The possibility of quenching the fluorescence of non-phagocytosed particles enables us to distinguish clearly and reliably between internalized and cell surface-bound particles.

Animals↗
PubMed1983

Macrophage function in high- and low-responder strains of mice.

Unstimulated peritoneal macrophages of the high-responder A/J mice compared to the low-responder B10 strain have a lower number of cells with the different Fc receptor (FcR) subtypes and correspondingly the FcR mediated phagocytosis is also lower. The intraperitoneal immunization with SRBC leads to a prompt increase of FcR expression and of phagocytic and pinocytic activity in the A/J mice, while in the B10 strain the activity of macrophages remains unchanged.

Animals↗
PubMed1983

Some properties of the plasma membrane of macrophages from germ-free rats.

The mobility of plasma membrane receptors for lectins concanavalin A (Con A), phytohemagglutinin (PHA), garden pea agglutinin (PSA), lentil agglutinin (LCA), peanut agglutinin (PNA), soybean agglutinin (SBA), wheat germ agglutinin (WGA), and perch spawn agglutinin (PFA), the presence of binding sites for sheep erythrocytes, the presence of Fc and complement receptors, as well as pinocytic and phagocytic activities were investigated in normal peritoneal macrophages from conventionally reared (CV) and germ-free (GF) rats. Differences varying according to the lectin used were found in lectin-receptor-complex lateral mobilities measured as a function of patch and cap formation. Germ-free-rat-derived macrophages showed a significant decrease in the average amount of SBA binding sites per cell as determined by 125I-SBA labeling. The percentage of complement- and Fc-receptor-bearing macrophages was lower in GF rats, in contrast to the higher percentage of macrophages forming spontaneous rosettes with sheep erythrocytes. The pinocytic activity as determined by the neutral red uptake assay exhibited a threefold increase in GF rat-derived macrophages in comparison to CV ones. On the other hand, phagocytosis was more intense in macrophages from conventional rats, as detected by the engulfing of CdCO3 microcrystals. Our results, together with other recent reports, indicate that the earlier opinion that the peritoneal macrophages of GF animals do not differ essentially from those of conventional ones needs to be revised.

Animals↗
PubMed1983

Phagocytosis of 2-hydroxyethylmethacrylate copolymer particles by different types of macrophages.

Phagocytic activity (for 2-hydroxyethylmethacrylate particles) in normal and thioglycollate- or proteose peptone-stimulated peritoneal macrophages, Peyer's patches macrophages, bone marrow macrophages and macrophage-like cell lines BWM, DCH 5, PU-5-lR and RAW 264.7, in suspension and after adherence to a substrate, was tested. Results of testing showed that 2-hydroxyethylmethacrylate particles are advantageous for following the phagocytic activity of the above cell types.

Animals↗
PubMed1982

Phagocytosis of human blood leukocytes: a simple micromethod.

A simple micromethod for testing human blood leukocyte phagocytosis employing synthetic hydrophilic particles based on 2-hydroxyethylmethacrylate is described. The normal level of phagocytosing leukocytes in healthy children was 20.1 +/- 2.7%, and in healthy adult donors 34.3 +/- 6.1%. The method was found suitable for routine testing in both clinical and laboratory practice.

Adult↗
PubMed1982

Changes in some properties and functions of plasma membranes of macrophages from young, adult and aged mice.

We studied age-dependent changes in distribution of receptor-ligand complexes in the lateral plain of plasma membrane after binding of six different lectins and anti H-2a and anti Ias antisera to the surface of mouse peritoneal macrophages. Additionally we examined formation of spontaneous rosettes with sheep red blood cells and the phagocytic and pinocytic activities of these macrophages. Macrophages of young and aged mice exhibited a lower mobility of the studied antigens and lectin receptors (determined as a function of patch and cap formation) and a higher rate of formation of spontaneous rosettes. The percentage of Ia antigens bearing macrophages was very low during early ontogenesis; it increased during adulthood and decreased during ageing. The highest percentage of phagocytizing macrophages was found in adult mice, even thought the phagocytic index (i.e., the average quantity of phagocytized particles in a phagocytizing cell) kept increasing during postnatal life. However, the level of pinocytosis was the same in all three groups.

Aging↗
PubMed1981

[Effect of diluents for long-term storage on the acrosomes of ram spermatozoa].

Samples of ram sperm, stored for a long time and diluted in a lactose-yolk or TRIS-glucose-yolk diluent in formulation currently used in A. I. practice in the Czech Socialist Republic, were subjected to morphological evaluation under optical and electron microscope. No visible differences between the diluents were observed under the optical microscope and the amount of acrosomal defects was just slightly higher than in the control fresh sperm. However, differences and changes were observed in the electron-microscopic pictures. When the former diluent was used, the percentage of acrosomal defects in the sperm stored for a long time was 44% and in the case of the latter diluent this percentage was as high as 59%, whereas in the control it was not higher than 16%. Most of the defects are caused during diluting and equilibration; less damage is suffered during freezing. The control osmometric determinations demonstrated a high hyperosmolality (495 mOsmol.kg-1) of the TRIS-glucose-yolk diluent. Hence the lactose-yolk diluent appears to be better, although the solubility of egg yolk in it is worse.

Acrosome↗
PubMed1981

Comparison of different methods for separation of human, mouse and rat macrophages and lymphocytes.

This paper gives a comparison of the following methods used for separating mouse or rat spleen peritoneal cells, and mononuclear cells from human peripheral blood: adherence to glass wool, repeated adherence to polystyrene, adherence to polystyrene with subsequent detachment of adherent cells by scraping, or by lidocaine or dispase treatment, adherence to polystyrene coated with foetal calf serum; and subsequent release of adherent cells by means of EDTA, adherence to Sephadex G-10 columns, phagocytosis of carbonyl nickel and subsequent separation on the Ficoll-Verografin mixture, or phagocytosis of silica particles. The techniques in italics seem most suitable for routine use.

Animals↗