Biomedical subjects

L G Belov

Publications and source records attributed to L G Belov.

5 recordsLinked to original sources

PubMed2000

Lipid peroxidation during experimental cholera intoxication.

Cholera intoxication in albino mice was induced by intraperitoneal injection of endotoxin in doses of LD(16), LD(25), and LD(50) and combination of endo- and enterotoxin in doses equivalent to LD(25). Dose-dependent activation of superoxide dismutase, phasic changes in the contents of MDA and conjugated trienes and dienes, and modulatory influence of enterotoxin on catalase activity in the blood were observed during intoxication.

Animals↗

PubMed1993

Identification of Yersinia pestis with varied plasmid composition using monoclonal and polyclonal fluorescent immunoglobulins.

The efficiency of serological identification of Yersinia pestis strains which contain different plasmids was assessed with polyclonal and monoclonal immunoglobulin preparations in the direct fluorescent antibody method. Plague polyclonal luminescent immunoglobulins recognize only those Y. pestis strains which contain pPst, pFra plasmids or both. Anticapsular plague monoclonal antibodies interact only with capsule-forming plague agent strains (pFra+) grown at 37 degrees C. With plague monoclonal lipopolysaccharide antibodies one can identify all Y. pestis strains irrespective of their plasmid content and cultivation temperature. However, these antibodies cross-react with Yersinia pseudotuberculosis bacteria in 60% of cases. The problem of laboratory diagnosis of the plague organism, whatever its plasmid profile, can be solved through the development of a test kit involving two preparations such as plague lipopolysaccharide monoclonal luminescent antibodies and pseudotuberculosis specific luminescent adsorbed immunoglobulins.

Animals↗

PubMed1991

[A micromethod of determining the activity of factor XIII in whole blood].

A simple, available, and sensitive micromodification of Sigg and Baluda's methods for measurements of Factor XIII activity is suggested with registration of the blood clot hemolysis degree in the urea. The method was tried in rabbits and guinea pigs during immunization against cholera. The results of Factor XIII activity measurements were found to be unrelated to blood fibrinogen level but they are in slight correlation with the blood fibrinolytic activity.

Animals↗

PubMed1988

[Interserovar antitoxic immunity to Vibrio cholerae toxins].

The immunochemical affinity of V. cholerae enterotoxins, serovars Inaba and Ogawa, has been shown in animal experiments on cross antitoxic immunity in the small intestine, the passive hemagglutination test and the toxin neutralization test. However, antitoxic interaction with both enterotoxins is characteristic only for antibodies to V. cholerae of serovar Inaba, while in animals immune to Ogawa toxin the choleragenic effect of enterotoxins produced by V. cholerae of both serovars in retained. The possible mechanisms of one-sided cross interserovar antitoxic immunity in cholera are discussed.

Animals↗

PubMed1988

[Interrelation between the blood neutrophil damage test and the passive hemagglutination reaction in detecting antibodies].

Statistical analysis of the results of examinations of vaccinees against plague has revealed that the values of the neutrophil damage index (NDI) and antibody titers as determined in the passive hemagglutination (PHA) test qualitatively (but not quantitatively) correlate. The statistical series for the PHA test and NDI belong to different variations, i. e. they describe different functions of antibodies with respect to the same antigen. Besides, the determination of NDI permits the detection of antibodies in 95% of cases with probability equal to 0.06, while the PHA test determines antibodies in 67% of the vaccinees with probability equal to 0.30. The determination of NDI, used as an alternative qualitative method for antibody detection, is particularly effective in the evaluation of faintly immunogenic antigens, as well as at the early and late periods of immune state.

Animals↗