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Biomedical subjects

L G Kindblom

Publications and source records attributed to L G Kindblom.

At least 109 records · Page 6Linked to original sources
PubMed1984

Fibroma of tendon sheath. A light and electron-microscopic study of 6 cases.

The clinical picture, the light and electron microscopic appearance, and the histochemical findings are described in six cases of fibroma of tendon sheath. The ages at onset for the four men and two women range between 16 and 54 years. The lesions were well circumscribed, rounded or oval, and had a diameter of about 1 cm. Light-microscopically the lesions consisted of a dense fibrocollagenous tissue with a variable degree of collagenization and cellularity, the peripheral zone often being most cellular and containing slit-like vascular spaces. Electron-microscopically two main cell types were identified: myofibroblasts and fibroblasts, the former dominating in cellular areas, the latter dominating in collagenized areas. The histochemical analysis indicated the presence of sulphated glucosaminoglycans within the collagenous matrix. Immunoperoxidase staining for factor VIII RAG-related antigen gave prominence to the endothelium of capillary-like blood vessels. The differential diagnosis against giant cell tumor of tendon sheath is discussed. The observations indicate that fibroma of tendon sheath is a distinct entity of probable reactive, non-neoplastic nature.

Adolescent↗
PubMed1984

The ultrastructure of alveolar and embryonal rhabdomyosarcoma. A correlative light and electron microscopic study of 17 cases.

An ultrastructural study is presented of 8 alveolar rhabdomyosarcomas and 8 embryonal rhabdomyosarcomas, and one mesenchymoma with predominantly rhabdomyoblastic differentiation. Thin and thick myofilaments and Z-lines or Z-like densities were found in all. Intermediate filaments were found in 4 alveolar and 4 embryonal rhabdomyosarcomas. A correlation was noted between the light- and electron microscopic appearance of the tumours regarding the degree of differentiation. A wide differentiation spectrum of the tumour cells was observed which could be correlated partly with normal fetal myogenesis. Ultrastructural differences were demonstrated in the alveolar and embryonal types. Electron microscopy is considered to play an important role in the characterization and diagnosis of rhabdomyosarcoma and is a valuable tool in the differential diagnosis.

Adult↗
PubMed1984

Nasal polyps with atypical stroma cells: a pseudosarcomatous lesion. A light and electron-microscopic and immunohistochemical investigation with implications on the type and nature of the mesenchymal cells.

A case of nasal polyposis with stroma cell atypia in a 33-year-old man is reported. The light microscopic appearance made a rhabdomyosarcoma strongly suspected because of the presence of polymorphous, acidophilic, rhabdomyoblast-like cells. The clinical course was that of an ordinary recurring inflammatory nasal polyp. The ultrastructural and immunohistochemical analysis showed the fibroblastic and histiocytic nature of the atypical cells as being of a probably reactive nature. The reported case illustrates the value of electron microscopy and immunohistochemistry in the evaluation of the true type and nature of atypical mesenchymal cells in pseudosarcomatous lesions.

Adult↗
PubMed1983

Myxoid chondrosarcoma of the synovial tissue. A clinicopathologic, histochemical, and ultrastructural analysis.

The first case of a myxoid chondrosarcoma, originating in the synovia of the joint and tendon sheaths of the ankle in a 37-year-old man, is reported. Light microscopically the tumor was similar to extraskeletal myxoid chondrosarcoma as previously described. The chondromatous nature of the tumor was further indicated by the histochemical analysis, which revealed the presence of chondroitin 4- and 6-sulphate in the tumor matrix. Ultrastructurally, chondroblast-like cells with dilated rough endoplasmic reticulum, abundant glycogen, some lipid droplets and numerous microvilli-like projections of the cytoplasm dominated. Also mitochondria-rich cells were observed as well as intermediate cell forms. Based on the observations in this case, the frequent occurrence of synovial chondromatosis, the assumed synovial origin of extraskeletal chondromas and the wide morphologic and functional spectrum synovial intima cells may cover, it is suggested that the myxoid chondrosarcoma originated from the synovial intima cells.

Adult↗
PubMed1983

Light and electron microscopic examination of embedded fine-needle aspiration biopsy specimens in the preoperative diagnosis of soft tissue and bone tumors.

A technique is described for the light- and electron microscopic examination of fine-needle aspiration biopsy specimens in the preoperative diagnosis of soft tissue tumors and bone tumors. The results obtained after studies on 18 soft tissue tumors, five bone tumors, and three metastatic carcinomas, thought to be primary soft tissue tumors, indicate important advantages to be obtained by using this method compared to conventional aspiration cytology: (1) it gives a very well preserved cellular morphology as seen both light and electron microscopically; (2) the preserved tissue structure and growth pattern is of importance for the diagnosis in many soft tissue and bone tumors; (3) some conventional histologic stainings can be applied giving additional information and an appearance familiar to the surgical pathologist; (4) the specimens can be saved in blocks and new sections made whenever necessary; and (5) the opportunity of studying the material under the electron microscope often helps to confirm a suggested diagnosis and may be necessary in some cases in order to arrive at a definite diagnosis.

Biopsy, Needle↗
PubMed1983

Clear-cell sarcoma of tendons and aponeuroses. An immunohistochemical and electron microscopic analysis indicating neural crest origin.

A clinico-pathological, light microscopic and immunohistochemical study of 15 clear-cell sarcomas, with an ultrastructural analysis of 6 of the tumors, is presented. The tumors showed a strong predilection for tendons and aponeuroses of the extremities in predominantly young and middle-aged people. The clinical setting, course and light microscopic appearance agree well with the original description by Enzinger (1965). Nine of the 15 patients developed metastases, most of them including lymph nodes, and 8 of the patients had died at the time of follow-up (median follow-up time 4.8 years). Reducing pigment was demonstrated within the cells of 2 tumors. Ultrastructurally the 6 tumors studied had a uniform appearance with characteristically rounded or oval tumor cells with a single nucleus containing one or two very prominent nucleoli, a light-staining cytoplasm with a moderate amount of organelles and a variable content of glycogen. Polymorphic melanosomes were seen in the cells of one of the tumors. External laminas enclosed groups of tumor cells and invested parts of individual tumor cells. With immunoperoxidase analysis for S-100 protein positive staining was observed in the vast majority of the tumor cells of all 15 clear-cell sarcomas. Metastases appearing in 9 of the 15 cases showed positive staining for S-100 protein. There was a strong staining of the cytoplasm and generally a weak and varying staining of nuclei. The immunohistochemical and electron microscopic findings indicate that clear-cell sarcoma is a homogenous entity among soft tissue sarcomas, of probable neural crest derivation.

Adolescent↗
PubMed1983

Enzyme histochemistry of malignant fibroblastic histiocytic tumors. A light and electron microscopic analysis.

The enzyme pattern of 13 cases of malignant fibrous histiocytoma (MFH) and 11 cases of myxofibrosarcoma (MFS), a malignant myxomatous soft tissue tumor of fibroblastic histiocytic origin, has been studied. 6 of the 13 MFHs were analyzed enzyme histochemically at the light microscopic level and 7 on the ultrastructural level; of the 11 MFSs 9 were analyzed enzyme histochemically at the light microscopic level and 2 on the ultrastructural level. Differences were observed in the subjectively estimated enzyme activity between low grade MFS and high grade MFS and MFH, and also between histiocyte-like and fibroblast-like tumor cells. Generally a strong reaction of oxidoreductase enzymes (NADH2-diaphorase, NADPH2-diaphorase, glucose-6-phosphate dehydrogenase) and hydrolytic enzymes (acid phosphatase and leucine aminopeptidase) was found in the high grade tumors and was usually higher in the histiocyte-like than in the fibroblast-like cells. Ultrastructurally acid phosphatase occurred predominantly in primary and secondary lysosomes and Golgi zones of the histiocyte-like cells. A strong reaction of alkaline phosphatase was found light microscopically in 2 of 5 MFHs and 5 of 9 MFSs. Ultrastructurally alkaline phosphatase was located along the cytoplasmic membrane of predominantly fibroblast-like cells in 3 of 7 MFHs and 1 of 2 MFSs. The results agree with the concept of two main cell types in MFH and MFS, fibroblasts and histiocytes.

Adult↗
PubMed1983

Malignant hemangiopericytoma with admixed glandular structures in breast and lung metastases. A light and electron microscopic and histochemical study of a case.

A 29-year-old woman with a malignant retroperitoneal hemangiopericytoma which metastasized to both breasts and the lung, is reported. The metastases in the breasts and the lung included numerous glandular structures. The light and electron microscopic, and histochemical analysis confirmed the diagnosis of hemangiopericytoma, and indicated that the glandular component was not a true part of the neoplasm but preformed glandular structures were included. The findings are discussed in relation to previous reports on low-grade leiomyosarcoma with glandular formation in lung metastases. The differential diagnosis against synovial sarcoma, which in monophasic forms may show a prominent pericytoma-like pattern, and in biphasic forms includes pseudoglandular structures, is discussed.

Adult↗
PubMed1982

Elastofibroma. A correlated light and electron microscopic study.

Four cases of elastofibroma located in the subscapular region of 3 men aged 66, 74, and 83 years, and a woman 70 years old are reported. A correlated light and electron microscopic study including ultrastructural examination of Verhoeff's iron-hematoxylin (VIH)-stained sections was performed. Light microscopically, the elastofibromas were characterized by connective tissue built up by collagen fibers and sclerotic masses mingled with numerous fibers and globules of elastin material. In one micron thick Epon sections these elastin fibers often revealed a central axis surrounded by a mantle composed of periodic segments giving them a necklace-like appearance. The ultrastructural findings of these elastin structures, stained with VIH, and the appearance of the stroma cells and their relation to the elastin indicate that elastofibroma is a non-neoplastic reactive lesion in which elastin material is synthesized by the stromal cells and predominantly laid down around preexisting elastic fibers.

Aged↗
PubMed1982

Immunohistochemical investigations of tumors of supposed fibroblastic-histiocytic origin.

The aim of this study was to localize alpha 1-antitrypsin, ferritin, and lysozyme by means of the indirect immunoperoxidase technique and to evaluate the significance of these antigens as markers of histiocytic differentiation in tumors of a supposed dual fibroblastic-histiocytic origin. The series comprised 31 malignant fibrous histiocytomas (MFH) of the pleomorphic, spindle cell, and myxoid types, four cutaneous fibrous histiocytomas, and four atypical fibroxanthomas, four dermatofibrosarcoma protuberans, and two osteoclastomas of bone. For comparison, 15 soft tissue sarcomas of various other types were examined. Of the MFHs of the pleomorphic type, 18 of 22 (82 per cent) were positively stained for alpha 1-antitrypsin and 12 of 22 (54 per cent) were positively stained for ferritin. Of the five MFHs of the spindle cell type, none was positively stained for alpha 1-antitrypsin, three were positive for ferritin, and one was positive for lysozyme. None of the myxoid variants (corresponding to grade I-II myxofibrosarcoma) was positively stained for either of the antigens. These results and the observations made on the cutaneous fibrous histiocytomas, atypical fibroxanthomas, dermatofibrosarcoma protuberans, and the various soft tissue sarcomas indicated that 1) alpha 1-antitrypsin is a valuable marker of histiocytic differentiation in both benign and malignant fibrous histiocytomas, 2) ferritin can be visualized in more than half of these fibroblastic-histiocytic tumors, and the presence of ferritin distinguishes the spindle cells of these tumors from fibroblasts of connective tissue and most fibrosarcomas, and 3) lysozyme, although a good marker of histiocytic differentiation in ordinary histiocytes and benign fibrous histiocytomas, is a poor marker of neoplastic histiocytes of malignant tumors. The results further support the concept that MFH is a tumor of a dual fibroblastic-histiocytic origin.

Ferritins↗
PubMed1982

Atypical lipoma.

In a previous clinico-pathologic and prognostic study of a Swedish national series of liposarcoma the designation atypical lipoma was suggested for a group of well-differentiated non-metastasizing liposarcomas characterized by only moderate nuclear atypia. In the present report, 21 new cases of atypical lipoma are described. The tumors were mainly composed of univacuolated fat cells without cellular or nuclear atypia, but also showed univacuolated fat cells with enlarged, moderately polymorphic, dark nuclei. In two of the tumors a few multivacuolated fat cells with scalloped nuclei were found. Small multinucleated cells with overlapping, peripherally arranged nuclei, reminiscent of so-called floret-like cells as in pleomorphic lipoma, could occasionally be seen. Areas of generally delicate linear or patchy fibrosis with atypical nuclei were a common finding. Two atypical lipomas were studied ultrastructurally. The ultrastructural findings suggest that the nuclear polymorphism is of a neoplastic nature rather than of a regressive changed. Nine atypical lipomas were located intramuscularly, two intermuscularly and ten subcutaneously. Five of the subcutaneous tumors and three of the inter-and intramuscular tumors recurred one or more times. Most of them, particularly the subcutaneous ones, had primarily been diagnosed as ordinary lipomas, but re-examination of the histological sections after one or more recurrence revealed overlooked nuclear atypia. Metastasis or dedifferentiation at recurrence was not seen in any case.

Adult↗
PubMed1982

Immuno-histochemical localization of myoglobin in human muscle tissue and embryonal and alveolar rhabdomyosarcoma.

An immuno-histochemical investigation of the presence and localization of myoglobin was performed on cardiac and skeletal muscle tissue and on 9 embryonal and 9 alveolar rhabdomyosarcomas, utilizing an immunoperoxidase technique. Cardiac muscle fibres were evenly stained whereas staining of skeletal muscle fibres varied, giving a mosaic-like pattern. Of the fixatives used (4 percent formaldehyde, 4 percent paraformaldehyde, formaldehyde-glutaraldehyde, and Bouin's fixative), 4 percent formaldehyde gave the most prominent staining; short fixation-time slightly increased the staining intensity. All the 9 embryonal and 9 alveolar rhabdomyosarcomas contained positively-stained tumour cells. The number of cells positively-stained and the intensity of the staining varied with differentiation: the most differentiated rhabdomyoblasts stained most intensely. However, also many poorly-differentiated tumour cells in highly cellular areas were positive. Myoglobin is considered a suitable marker of rhabdomyoblastic differentiation, thus the method used in this study may be valuable in the diagnosis.

Cell Differentiation↗
PubMed1982

Factor VIII related antigen and mast cells.

Using an immunoperoxidase technique for the demonstration of factor VIII related antigen, a hitherto undescribed observation was made that human mast cells are positively stained for this coagulation factor. Mast cells of both connective tissue and mucosa origin were positively stained. The observation is considered to support the opinion that mast cells may play a role in local haemostasis.

Antigens↗
PubMed1981

Malignant giant cell tumor of soft tissues: a clinicopathologic, cytologic, ultrastructural, angiographic, and microangiographic study.

A clinical, angiographic, pathologic-anatomic, and cytologic study of 12 cases of malignant giant cell tumor of soft tissues is presented. Preoperative angiography was performed in five cases, one of which was also studied by microangiography. Aspiration cytologic examination was performed in five cases and electron microscopy in one case. In nine cases the tumors were deeply situated and in three they were subcutaneous. In six cases the tumors were located within the lower extremity, in four cases in the upper extremity and shoulder, in one case retroperitoneally and in one case in the neck. Except for one case, the follow-up period was 1--14 years. Seven of the patients had died from metastasizing tumor disease at the time of follow-up. High vascularity and rapid circulation characterized the tumors at preoperative angiography. Conventional histology, aspiration cytology, and electron microscopy disclosed histiocyte-like cells, fibroblast-like cells, and multinucleated giant cells of both osteoclast-like benign and pleomorphic malignant type, in varying proportions. The histiocyte-like cells and the giant cells exhibited phagocytic activity at these examinations. The finding of phagocytosing pleomorphic malignant cells and giant cells of osteoclast type in aspiration cytologic material strongly favors the diagnosis of malignant giant cell tumor of soft tissues.

Adult↗
PubMed1981

Chordoma. A clinicopathologic and prognostic study of a Swedish national series.

Chordoma is a rare tumor with slow growth developing from remnants of the notochord and thus appears in close relation to the axial skeleton. A Swedish national series collected over a period of 13 years, comprising 51 patients with a follow-up time of 8-20 years, was studied. Histologically all tumors had a typical appearance except two, which had characteristics of "chondroid chordoma". Fifty-seven per cent of the tumors were located in the sacrococcygeal region, 27 per cent in the spheno-occipital region and 16 per cent in the vertebrae. The peak age incidence was in the 6th and 7th decades. Male: female ratio was 1:1. The main symptoms were pain and neurologic disturbances. Skeletal destruction was noted radiographically in most instances. Intratumoral calcifications were rarely seen. The treatment was surgery, radiotherapy or a combination of these. There was only one long-term survivor without evidence of disease, a patient operated on for cervical chordoma 14 years earlier. Six other patients lived 8-18 years after diagnosis with chordoma. All other patients were dead; 39 died of chordoma, five of unrelated causes. Distant metastases were noted in 29 per cent. Chordoma constituted 17.5 per cent of all primary malignant bone tumors of the axial skeleton. The yearly incidence was 0.51 per million inhabitants in Sweden.

Adolescent↗
PubMed1981

Malignant giant cell tumor of the uterus. A clinico-pathologic, light- and electron-microscopic study of a case.

A clinico-pathologic study of a 69-year-old woman with a malignant giant cell tumor of the uterus compatible with malignant giant tumor of soft tissues is presented. The diagnosis was based mainly on light- and electron-microscopic findings, including the demonstration of phagocytic activity of the neoplastic cells. The tumor metastasized to the lung and the patient died a short time after clinical onset. The classification of uterine sarcomas and the differential diagnosis are briefly discussed.

Aged↗
PubMed1981

Angiolymphoid hyperplasia with eosinophilia of the skin. Light microscopic and ultrastructural study of 4 cases.

A clinical, light and electron microscopic study of four patients with angiolymphoid hyperplasia with eosinophilia of the skin is presented. Light-microscopically, the lesions were characterized by the small size and superficial location, the proliferation of mostly delicate vessels with prominent endothelial hyperplasia and cellular areas without distinct vessel structures, but with infiltrates of eosinophilic leukocytes, lymphocytes and plasma cells. Ultrastructurally, a marked proliferation of both endothelial cells and pericytes could be demonstrated and the solid cellular areas were shown to be composed of solid vascular buds. A multi-layered basal lamina was enclosing pericytes and external surfaces of endothelial cells. The differential diagnosis is discussed and it is emphasized that this lesion may be misinterpreted as angiosarcoma.

Adult↗