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L G Kindblom

Publications and source records attributed to L G Kindblom.

144 records · Page 8Linked to original sources
PubMed1977

Myxofibrosarcoma: a pathologico-anatomical, microangiographic and angiographic correlative study of eight cases.

A correlated angiographic, microangiographic and histological study of eight myxofibrosarcomas is presented. Myxofibrosarcoma is a very recently described type of malignant soft tissue tumour characterized by a mucoid and nodular appearance, predominantly arising superficially in the extremities and trunk in elderly people. Microscopically, the lobulated and myxoid tumours in the present series showed a characteristic plexiform network of fine capillary-like vessels and occasional wide, sinusoidal, angulated vessels. On plain radiographs they were all dense and well demarcated. Angiographically the tumours appeared highly vascular, with irregular tumour vessels often arranged in a patchy or nodular fashion. All revealed signs of a rapid circulation. The microscopic and angiographic appearances were correlated, in two cases utilizing microangiography. In the presence of a superficially located tumour with high density on plain radiographs, marked vascularity, patchy or nodular opacities, and signs of rapid circulation on angiogram one should suspect this diagnosis.

Adult↗
PubMed1977

Myxofibrosarcoma. A study of 30 cases.

A series of 30 myxofibrosarcomas is described. These malignant soft tissue tumours are characterized by a mucoid and nodular appearance, a coarse plexiform capillary pattern, and they are mostly seen subcutaneously (26 out of 30) in the extremities (24 out of 30) and trunk (4 out of 30) elderly people. Histochemical studies, comprising staining with Alcian blue and toluidine blue at different pH's with and without preceding digestion with testicular hyaluronidase and with the Scott technique, indicated the presence of hyaluronic acid but not sulphated glycosaminoglycans as chondroitinsulphates. Myxofibrosarcoma is believed to belong to the general category of fibroblastic and histiocytic malignant soft tissue tumours. The median diameter of the tumours was 7 cm. They were divided into 4 grades according to cellularity, cell atypia and mitotic activity. The grade III and IV tumours showed pronounced atypia, often with the bi- and multinucleated giant tumour cells and occasionally with giant cells of Touton's type, suggesting a relationship to malignant fibroxanthoma. All of the patients were treated surgically and one received also pre- and post-operative irradiation. None of the 2 grade I myxofibrosarcomas recurred, while 2 out of 7 grade II tumours, 6 out of 10 grade III tumours, and 7 out of 11 grade IV tumours recurred once and up to 9 times. Metastasis appeared in 7 out of 30 patients; grade I tumours were not seen in any of these cases. By the time of follow-up after intervals ranging from 1 month up to 27 years, 14 patients had died; 6 of these had died post-operatively or of intercurrent disease. The differential diagnosis between myxofibrosarcoma and other myxoid soft tissue tumours is discussed.

Adult↗
PubMed1977

Relapsing polychondritis. A clinical, pathologic-anatomic and histochemical study of 2 cases.

A 57-year-old man and a 70-year-old woman with relapsing polychondritis are reported. The man, suffering from arthralgias, respiratory obstruction, external ear and sanddle-nose deformities, conjunctivitis and irido-cyclitis, died after 4 years from airway obstruction because of tracheal and bronchial collapse. The woman is alive 8 months after the development of respiratory obstruction, probably caused by radiographically demonstrated tracheal obstruction, a saddle-nose deformity and hearing impairment. Microscopically, the involved cartilages showed degenerative and slight inflammatory changes and were eventually replaced by fibrous tissue. Histochemical studies, utilizing staining with Alcian blue at controlled electrolyte concentrations (Scott technique) and at controlled pH:s, with or without digestion with bacterial chondroitinase ABC; and staining with the PAS-method, with or without diastase digestion, revealed a complete or relative loss of glucosaminoglycans and glycogen. A biosynthetic defect is considered unlikely to be the primary pathogenetic mechanism of relapsing polychondritis. Histological and histochemical examination of biopsies from involved cartilages contribute to a definite diagnosis.

Aged↗
PubMed1977

Disappearing bone disease. A clinical and histological study.

The case histories of three patients, a man and two boys, with disappearing bone disease are reported. The clinical, roentgenographic, and histopathological features are described in detail. Histologically, in the early stages of the disease, the vanishing bone is replaced by numerous wide engorged capillaries. Eventually the bone is replaced by dense fibrous tissue. All three patients were treated by radiotherapy. Histochemical studies performed in one case revealed strong acid phosphatase and leucine aminopeptidase activities in perivascular mononuclear cells (possibly pericytes), suggesting that these cells took part in the bone resorption.

Acid Phosphatase↗
PubMed1976

Primary carcinoma of the seminal vesicle. Case report.

A 72-year-old man with a seminal vesicle carcinoma is reported. The patient was treated by local excision of the tumour and radiotherapy. When the patient deteriorated and radiological and scintographic signs of skeletal metastases developed, hormone therapy (oestrogen) was initiated. Within a few weeks the patient was free from his severe pain. The radiological and scintographic signs of metastases had either diminished in size or disappeared after one year of oestrogen therapy. The patient is still alive and well 2 years after the diagnosis was established.

Adenocarcinoma↗
PubMed1976

Liposarcoma of the meninges. A case report.

A report on a liposarcoma in the meninges of a 70-year-old woman is presented. Pre-operative clinical and angiographic findings indicated that the tumour might be a meningeoma. Pathological examination revealed a predominantly lipoma-like, well-differentiated liposarcoma with round-cell and pleomorphic areas. The angiographic and pathological differential diagnosis are discussed.

Aged↗
PubMed1976

Spindle cell lipoma.

A clinical, light- and electronmicroscopic study of 14 patients with spindle cell lipoma is presented. Spindle cell lipoma is considered to be a distinctive lipomatous tumour histologically characterized by a mixture of fat cells and fibroblast-like spindle cells, ultrastructurally similar to fibroblasts, in a matrix with varying amounts of collagen and mucosubstances. The tumours showed a predominance for elderly men and all but one were situated in the posterior neck, shoulder region or upper back. The tumours varied between 1 and 9 cm, with a median value of 5 cm, in the widest diameter and were entirely or almost entirely situated in the subcutaneous tissue. A follow-up study of 11 patients, observed for 1-25 years, confirmed that the clinical course is benign. The differential diagnosis is discussed and it is emphasized that spindle cell lipoma is easily misinterpreted as sarcoma. Three tumours showed a pronounced nuclear polymorphism without mitotic activity, thought to be regressive in nature. The cellular change in these three tumours are presumed to be analogous with those in so called ancient neurilemmoma and therefore the name ancient spindle cell lipoma is proposed for the polymorphic spindle cell lipomas.

Adult↗
PubMed1975

Histochemical characterization of mucosubstances in bone and soft tissue-tumors.

The present investigation endeavors to characterize the mucosubstance content of 170 myxoid and chondromatous tumors and chordomas by histochemical methods. The results obtained using the critical electrolyte concentration (CEC) method as introduced by Scott and co-workers23,24 were compared with those obtained by staining with alcian blue and toluidine blue at different pH's with and without pretreatment with bovine testicular hyaluronidase. Tissues known biochemically to contain different heteroglycans were used as controls: synovial fluid and cock's comb (hyaluronic acid) stained with alcian blue up to a MgCl2 concentration of 0.1 M; fetal cartilage (chondroitin 4- and 6-sulphate) pulposus with notochordal remnants (keratan sulphate) up 10 1.0 M. The staining reaction of intramuscular myxoma and myxoid liposarcoma corresponded to that of synovial fluid and cock's comb (containing hyaluronic acid). Benign chondromatous tumors (osteochondroma, enchondroma, extraskeletal chondroma, chondromatosis in bursae, synovia, and tendon) as well as well-differentiated chondrosarcomas had a similar staining reaction to that of adult cartilage (containing keratan sulphate). However, the intensity of the reaction was lower in these tumors than in the adult cartilage, indicating that the keratan sulphate content of the tumors is lower. Most of the moderately well-differentiated chondrosarcomas, the poorly differentiated chondrosarcomas, and pulmonary metastases of chondrosarcoma, as well as mesenchymal chondrosarcoma and extra-skeletal chondrosarcoma possessed the same staining properties as fetal cartilage, known to contain chondroitin 4- and 6-sulphate but not keratan sulphate. A few of the moderately well-differentiated chondrosarcomas stained up to a MgCl2 concentration of 1.0 M. Three cases of poorly differentiated chondrosarcomas stained with alcian blue up to 0.35-0.45 M in the lowest differentiated areas, indicating the presence of sulphated heteroglycans, as chondroitin 4- and 6-sulphate. Most chordomas possessed the same staining properties as fetal cartilage; however, a few chordomas stained in the same way as notochordal remnants of nucleus pulposus (containing keratan sulphate), which are thought to be the origin of these tumors. The results of staining of the tumors in the present series with the Scott technique corresponds well with toluidine blue and alcian blue at different pH's with and without pretreatment of the sections with testicular hyaluronidase. Since bone and soft tissue tumors may contain varying mucosubstances depending on the tissue of origin and on differentiation, histochemical investigation of the heteroglycan content of these tumors may be a valuable diagnostic aid.

Adult↗
PubMed1975

Diffuse lipomatosis in the leg after poliomyelitis.

Two elderly women with gradual enlargement of the leg following paralytic poliomyelitis are reported. From the clinical appearance, plain radiograms and angiographic studies the enlargement was thought to be caused by adipose tissue. On exploration the muscles within the calfs were found to be entirely replaced by adipose tissue. Histological examination revealed diffuse lipomatosis involving skeletal muscle, fasciae and in one case even periosteum and bone. Peripheral nerves exhibited fibrosis and myelin degeneration and vessel walls showed unusual angioma-like changes. Since it is known from the literature that peripheral nerve lesions of different kinds can be followed by adipose overgrowth we believe that there is a pathogenetic relationship between poliomyelitis and the diffuse lipomatosis in the present cases.

Adipose Tissue↗
PubMed1975

Liposarcoma a clinicopathologic, radiographic and prognostic study.

The present investigation is a clinicopathologic, radiologic and prognostic study of 122 liposarcomas registered in the Swedish Cancer Registry between 1958 and 1966. The incidence of liposarcoma in Sweden was estimated to be 2.5 cases per million inhabitants per year. The liposarcomas were divided into 4 main subtypes according to WHO's recommended classification (1969): well-differentiated (33 cases), myxoid (22 cases), rundcell (14 cases) and pleomorphic types (21 cases). When predominantly well-differentiated or myxoid liposarcomas contained areas of round-cell or pleomorphic types (24 and 8 cases, respectively), they were classified separately, thus adding 2 further subtypes to our classification. The validity of this histologic classification was emphasized by the prognostic study, based on a 10 year follow-up. The differential diagnosis for each of the 4 main subtypes is discussed. Three well-differentiated, lipoma-like liposarcomas which contained highly vascular hibernoma-like areas are described. Two well-differentiated liposarcomas situated in the neck and upper part of the back, respectively, of elderly patients included area similar to "spindle-cell lipoma" (Enzinger). An intracytoplasmic network of birefringent fibers with the staining reaction of collagen was found in 2 well-differentiated fibrosing liposarcomas...

Adolescent↗
PubMedDate not supplied

A comparative study of enzyme histochemical features in the gerbilline and human cholesteatoma.

Spontaneous and experimentally induced cholesteatoma in the Mongolian gerbil has been found to exhibit histopathological similarities to human aural cholesteatoma and has been suggested as an experimental model for studies of the clinical situation. In an attempt to further characterize this model, we compared experimentally induced cholesteatomas in the external auditory canal from gerbils with those of the human ear by means of a correlated histopathologic and enzyme histochemical study. The human and gerbilline cholesteatomas revealed similar histopathologic features. Even enzyme histochemically, the human and experimentally induced cholesteatomas demonstrated similar features. Thus glucose-6-phosphate dehydrogenase activity, an indicator of oxidative metabolism, was demonstrated especially in the stratum granulosum cells of the heavily orthokeratinizing squamous epithelium adjacent to the cholesteatomas. The human ear canal skin also revealed enzyme histochemical characteristics similar to the squamous epithelium lining the human cholesteatoma. The hydrolytic enzyme activity (leucyl-aminopeptidase) was strong in the connective tissue surrounding human cholesteatoma when compared with that of ear canal skin. In the gerbilline cholesteatoma, this activity was demonstrated especially in the connective tissue adjacent to eroded bone, which possibly may facilitate cholesteatoma progression. We conclude that experimentally induced cholesteatoma has both histophatological and enzyme histochemical similarities to human aural cholesteatoma and therefore it is suggested that the gerbilline model may be used for studies on the development of human cholesteatoma. Our results support the view that cholesteatoma may originate from migrated hyperkeratinizing cells from the epidermis of the tympanic membrane or the meatus.

Acid Phosphatase↗
PubMedDate not supplied

A rapid and simple method for electron microscopy of paraffin-embedded tissue.

A new rapid and simple method for electron microscopy of paraffin-embedded material is described. The paraffin-embedded tissue is deparaffinized in xylene, stained in a 0.01% toluidine blue/absolute ethanol solution, infiltrated in a propylene oxide/resin mixture, and embedded in epoxy resin. This quick method, which excludes rehydration, postosmification, and dehydration, takes about 3 h and produces results equal to previously described more laborious re-embedding techniques.

Humans↗
PubMedDate not supplied

Agarose embedding: a new method for the ultrastructural examination of the in-situ morphology of cell cultures.

A new technique for the ultrastructural examination of the in-situ morphology of cell cultures is described. By embedding the cell cultures in situ in agarose before embedding them in epoxy resin, numerous agarose blocks with the cells on top of the blocks can be obtained and used for different types of investigation, including electron microscopy. Use of this technique enables many of the problems encountered with earlier methods to be avoided.

Animals↗