Biomedical subjects

L G Stoliarova

Publications and source records attributed to L G Stoliarova.

3 recordsLinked to original sources

PubMed1978

[Effect of dimethyl sulfoxide on transformation of B. subtilis in vitro].

Bacillus subtilis transformation was conducted in the presence of dimethylsulfoxide and polyethyleneglycol. B. subtilis transformation was most frequent under the effect of 0.1% dimethylsulfoxide and was not altered significantly by polyethyleneglycol. As suggested, an increase of the transformation frequency was associated with the change of the membrane permeability under the influence of dimethylsulfoxide of with the altered activity of the membrane DNase.

Bacillus subtilis↗

PubMed1977

[Conduct of Bacillus subtilis transformation in mice under conditions of immunologic suppression of exogenous DNAase 1 activity].

Bacillus subtilis transformation was conducted in the abdominal cavity of mice. The frequency of transformation was considerable decreased when bovine DNA-ase 1 (3-- 5microgram) was injected intraperitoneally to these animals. Immune rabbit gamma-globulins containing antibodies to bovine DNA-ase 1 inhibited in vivo the activity of DNA-ase 1, protected transforming DNA from the hydrolyzing effect of this enzyme. This model can be utilized in search for ways to preserve DNA injected into the animal organism for the purpose of genetical engineering.

Animals↗

PubMed1977

[Antigenic properties of genetic recombinants of serologically typed E. coli].

The conjugation between the typed strains of E. coli belonging to various serological groups and conjugation between typed and untyped E. coli strains were studied. Genetic determinant controlling the synthesis of the O100 antigen proved to be closely linked with histidine locus. Among recombinants obtained in crossing the typed E. coli strains there were such belonging to the serological type different from the serological types of donor and recipient cells.

Antigens, Bacterial↗