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Biomedical subjects

L García

Publications and source records attributed to L García.

At least 163 records · Page 9Linked to original sources
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Bovine respiratory syncytial virus: first serological evidence in Uruguay.

Bovine respiratory syncytial virus (BRSV) is a major cause of respiratory disease in calves resulting in a substantial economic loss for the cattle industry worldwide. In order to determine the presence of BRSV in Uruguay, an immunoenzymatic test was set up, using a recombinant BRSV nucleocapsid (N) protein as the antigen. The N protein was produced in Sf9 insect cells by a recombinant baculovirus expressing the N protein. Serum samples collected from one hundred cattle from four different geographic regions of Uruguay were analyzed. Antibodies against the N protein of BRSV were detected in 95% of the serum samples analyzed. These results show for the first time the presence of BRSV antibodies and suggest a widespread BRSV infection in the cattle population of Uruguay.

Animals↗
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Emboli in bulls killed in Spanish traditional bullfighting.

The finding of brain tissue fragments in blood and lungs of cattle stunned in slaughterhouses has raised concerns about food safety in the context of the bovine spongiform encephalopathy epidemic. In the present study, the possible occurrence of brain tissue emboli in animals killed in traditional Spanish bullfighting was investigated. Thorough histological analysis of multiple possible target organs was carried out in 434 bulls. No evidence of brain tissue embolism was obtained, but emboli from diverse sources were detected in pulmonary and hepatic tissue of a significant number of animals. These emboli seem to have been caused by the use of a long sword, which extensively disrupts intra-thoracic and intra-abdominal organs and vascular structures.

Animals↗
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[Amebic liver abscesses with bacterial superinfection in a nonendemic area].

A 26-year-old man was admitted to hospital with asthenia, weight loss, right upper quadrant abdominal pain, diarrhea without blood, and fever. Abdominal ultrasonography showed multiple hypoechoic areas in the left hepatic lobe. On abdominal CT, multiple hypodense areas without contrast capture were consistent with hepatic abscesses. Cultures were obtained and the patient was placed empirically on metronidazole, gentamicin and ampicillin, without improvement in the first 72 hours, and a drain was placed in the largest lesion collecting a purulent material. The abscess culture was positive for group C beta-hemolytic Streptococcus. Entamoeba histolytica serology was positive and colon biopsies revealed trophozoites. Multiple left hepatic lobe abscesses secondary to E. histolytica, with bacterial superinfection, is an unusual presentation of amoebic infection, considering that Spain is not an endemic area.

Adult↗
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[Validation of the Panic Attack Symptoms Scale].

INTRODUCTION AND METHOD: The ESAP is a self-rating scale based on the panic attack with 0 to 3 scoring and the total score is obtained by adding the 14 scores- the highest score thus being 42. Forty patients were included for the study, and the were assessed at their first visit, after one week and after one month in the treatment. RESULTS: The items at the first basal assessment followed a normal distribution (Test Shapiro-Wilk w: 0.97, p< 0.57). The mean score was 18.5 with a SD of 7.43. The scale's internal consistency was measured by Cronbach's alpha, which was 0.66 at the first visit, 0.60 at the second one and 0.90 at the third one. Test-retest reliability was good in most cases, with values greater than 0.7 (p< 0.001). The correlation between the first visit total score and the second one was 0.89 (p< 0.001). The correlation with other scales (concurrent validity was highly significant with Hamilton's anxiety scale, total PASS and most of the PASS sub-scales (unexpected panic, limited and anticipatory anxiety) and slightly significant with the number of crises in past month. Four factors accounting for 65% of variance were identified in the factor analysis.

Adult↗
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[Causes of failure in psychopharmacological treatment of anxiety disorder].

OBJECTIVE AND METHODS: A comprehensive review of literature on the causes of failure in pharmacologic treatment of panic disorder (PD). RESULTS: PD has high rates of response to psychopharmacological treatment, reaching up to 80-90%. However, in a small percentage of cases (10-20%), the disorder is refractory to the combination of drugs and psychotherapy, at correct dosages and during adequate time periods. Refractoriness in PD is not a well-known issue as few studies have addressed this question. Intolerance to psychotropic drugs is usually reported to be the commonest cause, especially to antidepressants. Other causes are: comorbidity (especially with depressive disorders, personality disorders, and alcohol abuse, as well as with medical conditions); severe panic attacks; severe agoraphobia; and long illness duration. < > PD is to be ruled out, since incorrect diagnosis, incorrect treatment, or bad compliance are common causes of a poor response. We review treatment approaches for PD when no response is achieved with antidepressants (TCA, SSRI, MAOI), alprazolam and cognitive-behaviour therapy. Two recommended strategies are drug potentiation and use of clonazepam.

Antidepressive Agents↗
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Matrix metalloproteinase profile in patients with Creuztfeldt-Jakob disease.

Preliminary findings suggest that abnormalities in matrix metalloproteinase (MMP) activity may be found in the cerebrospinal fluid (CSF) of patients with Creutzfeldt-Jakob disease (CJD). In this study of 16 subjects with CJD and 16 age-, and sex-matched controls, we determined the presence of MMP-2 and MMP-9 in their active and proenzyme forms, the relative levels of MMP-3 and four inhibitors of MMP activity (TIMP-1, TIMP-2, TIMP-3 and TIMP-4), and the concentration of 4-3-3 protein. The methodology used involved zymography and immunological techniques. The results indicate that, compared with controls, CJD patients have a significantly higher positive frequency of pro-MMP-9 and of the active form of MMP-2, along with significantly higher levels of TIMP-1 and TIMP-2, classical inhibitors of MMP-9 and MMP-2, respectively. We also found a positive correlation between 14-3-3 protein concentration and that of TIMP-1 and TIMP-2 levels (correlation coefficients of 0.793 and 0.798, respectively). These results suggest that abnormalities in MMP and TIMP profiles may be helpful in the biochemical characterisation of CJD.

14-3-3 Proteins↗
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[Cutaneous hemangiomas and vascular malformations and associated pathology (Pascual-Castroviejo type II syndrome). Study of 41 patients].

AIM: To describe the clinical, diagnostic and therapeutic features of this angiomatous neurocutaneous syndrome, which is the most frequent one, and to report a personal series of 41 patients. PATIENTS AND METHODS: Forty one patients--31 females and 10 males--were studied during childhood and then, several patients were followed during many years, which allowed us to learn about the evolution of the abnormalities. The cutaneous lesions were classified as hemangiomas in 30 patients (73%) and as vascular malformations in 11 patients (27%). RESULTS: A cerebellar anomaly (unilateral hemispheric hypoplasia and Dandy-Walker malformation) was seen in 13 patients (31.5%) cerebral cortical dysplasia in 4 patients (10%), aortic arch coarctation in 6 patients (15%), and congenital cardiopathy in 5 patients (12%). The most frequent abnormalities were intracranial and/or extracranial vascular malformations. Persistence of the trigeminal artery was observed in 7 patients (17%), absence or severe hypoplasia of an internal carotid artery in 13 patients (32%), absence of a vertebral artery in 7 patients (17%), hypoplasia of intracranial arteries in 6 patients (15%) and aneurysmal enlargement of carotid or vertebral arteries in 5 patients (12%). Also were observed 4 patients (10%) with intracranial hemangioma, 2 (5%) with hemangioma in mediastinum, and 3 (7.5%) with intestinal hemangioma, all of which disappeared during the first years of life. Aneurysmal enlargement of the carotid and vertebral arteries and intracranial branches also disappeared after a process of progressive narrowing of the arterial lumen that caused complete obstruction of these arteries. At the same time the cutaneous hemangioma regressed. During this process, collateral vascularization through branches of the external carotid artery and of the non-affected branches of the contralateral intracranial arteries developed. CONCLUSION: This neurocutaneous syndrome is the most frequent one and it is associated with several types of vascular and non-vascular abnormalities which can involve any organ of the body. Internal and external hemangiomas and hemangiomatous lesions progress and tend to regress concomitantly.

Cardiovascular Abnormalities↗
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[Spontaneous pneumopyelography: ureterocolic fistula caused by rectosigmoid carcinoma].

We present a case of ureterocolic fistula, secondary to a rectosigmoid carcinoma. The infrequency of these ureterocolic fistulae, contrasts with the more usual vesicointestinal ones. In our case, the diagnosis of pathological communication arose from the presence of gas in the excretory tract, after ruling out all those processes which could have led to a similar situation. In agreement with the world literature consulted, we confirm the great difficulty in tracing the course of the fistula by the usual means of diagnosis, in view of the minimal size of the pathological communication and the frequent obstructive behaviour of the fistula itself.

Adenocarcinoma↗
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[Clinical subgroups of anxiety disorders: from phenomenology to cluster diagnosis].

The panic disorder is heterogeneous. The factorial study of the phenomenology of panic crisis suggests the existence of different subtypes: the cardio-respiratory, the vestibular, the despersonalization, the gastrointestinal and others. We review the clinical and biological data which suggest the display of these subtypes.

Adult↗
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Troublesome diagnosis in two children born to HIV-1 infected mothers.

In order to assess early HIV infection vertically transmitted in children it is necessary to use techniques that directly detect HIV. Positive results were found in some rare cases who later seemed to have cleared the infection. We communicate two cases of children with troublesome diagnosis. Two girls born to HIV-1 infected mothers were followed-up since birth up to 40 months old, with viral culture, polymerase chain reaction (PCR), p24 antigen detection and serologic techniques. PCRs were positive in three opportunities at 2, 8 and 30 months of age in the first child and confirmatory tests for specific antibodies remained indeterminate up to the age of 34 months. Positive viral DNAs were detected in two opportunities in the second child at 2 and 4 months of age. Western Blots were negative since 25 months. No virus was recovered nor was p24 antigen detected during the whole period of study in either child. Sequestration of the virus in lymphatic tissue, low replicative ability of the virus and/or immunological tolerance can be postulated in the first case. In patient 2, it could be hypothesized that infection, if any, had cleared up.

Adult↗