A rapid gas chromatography-mass spectrometry method for the determination of urinary 3-methyladenine: application in human subjects.
A rapid gas chromatography-mass spectrometry method, employing immunoaffinity clean-up, has been developed for the measurement of 3-methyladenine in human urine samples. A wide variation in levels of urinary 3-methyladenine was observed, indicating that at least some may be derived from the diet and not be related to endogenous nitrosation and subsequent methylation.