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L Geerts

Publications and source records attributed to L Geerts.

28 records · Page 2Linked to original sources
PubMedDate not supplied

Techniques of sperm selection, improvement and separation in an in vitro fertilization program.

The Authors present a review of the various techniques used for improving the fertility potential of sperm within the context of an in vitro fertilization (IVF) program. After a brief description of the mechanisms leading to normal in vivo fertilization, they discuss the different methods of selecting and improving sperm for IVF. They conclude that centrifugation on discontinuous Percoll gradients would seem to be the most efficient separation method from all points of view, while the addition of pharmacological agents to improve sperm quality and motility lead to extremely unsatisfactory results.

Cell Separation↗
PubMedDate not supplied

Within-subject variations of sperm parameters and their importance in an in vitro fertilization program.

Spermiograms of 150 males were assessed, in view of studying them before an in vitro fertilization attempt. Within subject variations were determined with regard to sperm count and spermatozoal motility pattern. Ultimately these results were compared to the seminological parameters studied at the time of the in vitro-fertilization attempt. Overall results showed a clear drop in semen parameters at the time of egg retrieval. This drop was more marked when semen parameters were amongst the higher values at the start.

Humans↗
PubMedDate not supplied

Pregnancy obtained with human testicular spermatozoa in an in vitro fertilization program.

The fertilizing capacity of human testicular spermatozoa and the positive outcome of an in vitro fertilization program open a wide range of opportunities for men suffering from obstructive and inoperable azoospermia. In six cases, subzonal sperm injection or intracytoplasmic sperm injection techniques were applied to inject testicular spermatozoa into human oocytes. The fertilization rate after testicular sperm injection reached 45%. Normal cleavage was observed and replacement of 10 embryos in 6 patients resulted in one chemical and one ongoing pregnancy.

Cell Separation↗