Reverse-phase high performance liquid chromatography on a microBondapak C-18 Column has been used to separate and quantify acetanilide and its aromatic monohydroxy derivatives in the 2-, 3- and 4- positions. Separation was achieved within 22 min by using an isocratic mixture of 2-propanol: methanol: water, 8:18:74 (v/v). This method compares very favourably with other HPLC techniques already reported to separate acetanilide from the monohydroxy aromatic derivatives.

Acetaminophen↗

PubMedDate not supplied

Modulation of rat liver aryl hydrocarbon (benzo[a]pyrene) hydroxylase activity by nutritional effects.

Rats malnourished since birth and fed a protein-free diet for 2 wk showed almost undetectable levels of liver microsomal aryl hydrocarbon (benzo[a]pyrene) hydroxylase. Treatment with benzo[a]pyrene rapidly enhanced this activity to levels higher than those observed with untreated normal rats. The carbon-monoxide-reduced cytochrome P-450 spectral peak was shifted from 452 nm in malnourished untreated rats to 448 nm in malnourished benzo[a]pyrene-injected rats and resulted in increases in the intensity of several microsomal protein bands (MW range 46,000-60,000) separated by gel electrophoresis. Malnourished rats then fed with a protein diet exhibited an important increase in aryl hydrocarbon hydroxylase activity, an increase in the intensity of microsomal protein electrophoretic bands (MW range 46,000-60,000), and a shift of the carbon-monoxide-reduced cytochrome P-450 spectral peak from 452 nm to 450 nm. These results suggest that alterations in cytochrome P-450 species related to benzo[a]pyrene metabolism might explain the modulation of this activity by nutritional effects.

Animals↗

PubMedDate not supplied

[The evaluation of oxidative stress in a patient with transient cerebral ischemia].

Explore the source record for details and available documents.

Antioxidants↗

PubMedDate not supplied

[Use of information in evaluating team work in a peripheral outpatient clinic].

Explore the source record for details and available documents.

Ambulatory Care Facilities↗

PubMedDate not supplied

Skilled nursing facilities as a setting for comprehensive care of older people with advanced cancer: a model of care in the post oncologic stage.

METHODS: We surveyed the elderly cancer patients admitted to the Skilled Nursing Wards of Shmuel Harofe Hospital, Geriatric Medical Center, during a 5-year period. RESULTS: The 304 elderly patients admitted with advanced cancer represented 16% of the skilled nursing patients admitted during this period. Their average time of survival was 4.2 months, ranging from 9.1 for breast cancer to 1.8 months for gastric cancer. Ninety-five percent were severely dysfunctional, and all had a high rate of medical complications. CONCLUSIONS: Our study reveals the benefits of a skilled nursing system that provides comprehensive care, flexibility, and continuity of care at a difficult and demanding stage of this illness. This model functions at reasonable cost, and we recommend it for the care of elderly patients with advanced cancer in the post oncologic stage.

Journal Article↗

PubMedDate not supplied

Effect of the extender supplement Equex-STM on cryopreserved semen in the Assaf sheep.

This study was designed to evaluate the effect of adding the detergent Equex-STM to the extender used to dilute semen for cryopreservation on several indicators of sperm preservation. Two consecutive ejaculates per day were obtained from 5 Assaf sheep on two days out of every week over three alternate months. The freezing protocol involved diluting the semen in Fiser's extender, to which 0.7 percent Equex-STM was added or omitted before cryopreserving the semen in straws by exposure to nitrogen vapor. Equex-STM supplementation gave rise to significantly (p=0.05) improved sperm quality variables after different periods of freezing (0 hours, 1 week and 1 month). The variables examined were: individual motility, viability, acrosome integrity, plasma membrane integrity (HOS test) and morphological anomalies. This improvement was independent of the ram and month of testing. In a second experiment in which we incubated the semen (0 and 6 hours) at 37 degree C after thawing, Equex-STM also showed a beneficial effect on sperm quality.

Animals↗