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L Gimeno

Publications and source records attributed to L Gimeno.

16 recordsLinked to original sources
PubMed2004

Ezrin gene, coding for a membrane-cytoskeleton linker protein, is regionally expressed in the developing mouse neuroepithelium.

Ezrin is a member of the Ezrin, Radixin, Moesin (ERM) proteins family that are proposed to act as linkers between the cytoskeleton and plasma membrane. Ezrin regulates cell-cell and cell-matrix interactions playing a role in the regulation of cellular adhesion, movement and morphology in epithelia. Alterations in the expression of Ezrin and other members of ERM family have also been observed in brain tumours. Here we report the expression pattern of Ezrin during mouse neural development, from early stages to postnatal stages. In young and middle gestation embryos, Ezrin is expressed in the roof plate of the neural tube, in the presumptive domain of the choroidal plexus, and in some precise domains of ventricular epithelium. These domains are distributed in basal and alar neuroepithelial regions, some of them in relation to the expression of cadherins. At later gestation and postnatal stages, Ezrin expression is maintained on the mature choroidal plexus and is weakly detected in the proliferative regions of the mature brain.

Animals↗
PubMed2003

Study of Fgf15 gene expression in developing mouse brain.

Fibroblast growth factor 15 (Fgf15) is a gene regulated by the expression of Otx2 in developing mouse brain (Proc Natl Acad Sci USA 97 (2000) 14388). Otx2 gene codes for a transcription factor and is fundamental for the regionalisation and development of the anterior neural plate and cephalic region of the vertebrate embryo (Development 124 (1997) 3639). In addition, the thalamic expression of Fgf15 has been recently reported under the control of Shh signalling gene, expressed in the diencephalic basal plate (Development 129 (2002) 4807). In the present work, we have analysed Fgf15 expression pattern during mouse neural development. Fgf15 appeared early in the developing neural epithelium, in domains where Fgf8 gene is also expressed and, at later stages, in specific groups of neural cells. Fgf8 is an important signalling protein with demonstrated morphogenetic activity in several embryonic regions. Fgf15 expression is localized, like Fgf8, in secondary neural tube organizers: the isthmic organizer (IsO) and the anterior neural ridge (ANR).

Animals↗
PubMed2001

How effective has been the reduction of SO2 emissions on the effect of acid rain on ecosystems?

The paper attempts to assess the effectiveness of the reduction of SO2 emissions over recent years for protection of ecosystems in both Europe and eastern USA by analysis of temporal changes in the acidifying potential (AP) of wet deposition defined as [SO4(2-)] - ([Ca2+] + [Mg2+]). Spatial and temporal patterns of acidifying potential were studied. The main result is that there is no statistical evidence for a trend towards improvement in the acidifying potential, because there have been declines in Ca precipitation. This suggests that the reduction of SO2 emissions has not been effective as an abatement strategy.

Acid Rain↗
PubMed2001

[Urologic complications in renal transplantation. Study of 250 cases].

OBJECTIVE: The aim of this study is value the incidence of urological complications (fistulae and estenosis) in our serie of renal transplant, to analyze the variables that can influence in their appearance and the treatments used. Likewise, to value the follow-up of theses patients and the survival of the renal graft and of the patient. MATERIAL AND METHODS: 250 renal transplants are carried out between july of 1985 and october of 1998. The relationship among the variables you makes by means of the test chi 2 of Pearson and the test of Fisher; the contrasts of stockings with the t of Student; the survival of the organ and of the patient, by means of the analysis of curved of survival according to the method of Kaplan and Meier; and the comparison among curved of survival was carried out with the test of Cloth and Cox. RESULTS: Of the 250 transplants, 46 patients suffered for complications, 29 urinary fistulae (11.6%) and 21 estenosis (8.4%). The most frequent localization in both complications was the union uretero-vesical. The presence of urinary fistulae didn't influence negatively in a significant way nor over the survival of the implant (p < 0.211), neither over the patient's survival. The estenosis appearance was related in a significant way with the donor's age (p < 0.02). The estenosis presence was not related in a significant way neither with the survival of the implant neither with that of the receiver. CONCLUSIONS: The incidence of urological complications was of 18.4% (11.6% estenosis and 8.4% fistulae). The most frequent localization was the union uretero-vesical. The presence of estenosis correlated with the increase of the age of the donors. The technique of reimplante ureteral didn't influence in the results in a significant way. We don't find any relationship between the appearance of urological complications and the patient's survival.

Adolescent↗
PubMed2000

Therapy of visceral leishmaniasis in renal transplant recipients intolerant to pentavalent antimonials.

Visceral leishmaniasis should be suspected in renal transplant recipients in whom a fever develops of unknown origin. A 53-year-old renal transplant recipient developed pyrexia, hepatosplenomegaly, and pancytopenia 4 years after transplantation. Antileishmaniasis serology was negative, and the diagnosis was confirmed through bone marrow examination. Treatment with glucantine (N-methylglucamine antimoniate) led to acute pancreatitis, and treatment with ketoconazole plus allopurinol for 21 days was effective to eradicate Leishmania donovani.

Antimony↗
PubMed1998

The ideal therapy must be defined in each geographical area: experience with a quadruple therapy in Spain.

BACKGROUND: Multiple therapeutic combinations have been tested to determine the optimal regimen(s) for Helicobacter pylori eradication, leading to very different results depending on the geographical area. Our goal was to evaluate the efficacy of a "quadruple" therapy with omeprazole, tetracycline, bismuth and metronidazole in our area. MATERIALS AND METHODS: We investigate 106 consecutive patients with active peptic ulcer disease (duodenal, gastric or both) and Helicobacter pylori infection. One-week therapy with omeprazole 20 mg b.i.d., tetracycline hydrochloride 500 mg q.i.d., colloidal bismuth subcitrate 120 mg q.i.d., and metronidazole 250 mg t.i.d was prescribed. Between the days 30 and 40 after treatment ended follow-up endoscopy was performed. Eradication was defined as both negative urease test and histology. Between days 90 and 360 a 13C urea breath test was performed in 100 patients. RESULTS: Of the 106 patients in the study, 91 had duodenal ulcer, 12 had gastric ulcer, and 3 had both. Side effects were observed in 25% of the cases. Eradication was achieved in 87.7% (93/106; CI 79.9-93.3). Healing was obtained in 95.2% (100/105; CI 89.2-98.4); 97.8% (CI 92.4-99.7) in those eradicated and 75% (CI 42.8-94.5) in non-eradicated (p < .01). CONCLUSIONS: Quadruple therapy with omeprazole, tetracycline, bismuth subcitrate and metronidazole achieves healing rates up to 95-100%. The 87.7% eradication rate obtained suggests that the regimen we used is a reasonable therapeutic alternative in our area.

Adult↗
PubMed1996

Eradication of helicobacter pylori among patients from a primary care practice.

BACKGROUND: The eradication of Helicobacter pylori is becoming the therapy of choice for peptic ulcers, if the infection is present. Published data from primary care settings are, however, limited. METHODS: An open-ended, prospective study was undertaken that included 31 patients with active peptic ulcer demonstrated by endoscopy and H pylori infection confirmed by urease and histologic tests. After a 14-day period of treatment with omeprazole, bismuth, tetracycline, and metronidazole, healing and H pylori status were evaluated by repeat endoscopy done at least 28 days after the last treatment dose. Eradication is defined as absence of H pylori in at least four (two from the fundus and two from the antrum) samples taken from the gastric mucosa and a negative urease test. Drug side effects and patient compliance were monitored in all cases. RESULTS: Twenty-eight patients completed the protocol. Healing was obtained in all cases, and eradication was accomplished in 25 (89%). Side effects were common (69%) but mild. Compliance was good. After a mean follow-up of 300 days (range, 180 to 400), one ulcer recurrence was observed in an H pylori-positive patient and none in H pylori-negative patients. CONCLUSIONS: The treatment of H pylori infection is an effective way of healing peptic ulcers, and can be applied in primary care settings. Further studies with more patients and with shorter and easier therapies should be undertaken to confirm our findings.

Adult↗
PubMed1996

[The value of symptoms in the diagnosis of peptic ulcer: an approximation to the primary care medium].

GOAL: To evaluate which clinical data are useful to select those patients with peptic ulcers in primary care most likely to benefit from complementary studies. METHODS: This is a prospective study done on 101 patients evaluated in a period of a year in a Primary Care Center. In all patients an endoscopy was done when a peptic ulcer was considered a possibility. In all cases a standardized questionnaire was completed before endoscopy and the patient evaluated by his primary care physician. The final diagnosis was defined according to endoscopy, done by expert endoscopists within seven days of the clinical evaluation. Statistical analysis was undertaken with SPSS software. RESULTS: An active peptic ulcer was found in 45 (44.5%) cases. A high-grade MALT lymphoma was diagnosed in one case. Male sex, smoking status, number of cigarettes, smoking-index, and a previous history of ulcer complications were significantly associated with the diagnosis, as well as severe diurnal or nocturnal pain. Mean age was lower in ulcer patients. However no clinical data in individual or combined form did show any predictive value. CONCLUSIONS: Clinical data do not permit to obviate endoscopy as the key initial procedure to diagnosis, even in primary care.

Adult↗
PubMed1995

Pesticide effects on eel metabolism pesticide; levels did not decline at any time when animals were exposed to 4.1 mg/liter.

Previous works on endosulfan eel toxicology in this laboratory demonstrated that 0.041 mg/liter of endosulfan was the 50% lethal concentration of 96 hr exposure. Eels of species Anguilla anguilla were exposed to two sublethal endosulfan concentrations: 8.2 micrograms/liter (1/5 LC50) and 4.1 micrograms/liter (1/10 LC50), and the experiment was done at different exposure times: 12, 24, 48, 72, and 96 hr. Muscle glycogen content decreased significantly (p < 0.05) at 24, 48, 72, and 96 hr exposure to 8.2 micrograms/liter. Muscle lactate levels in fish did not change significantly while lactate levels in eel blood increased at 12, 24, 48, 72, and 96 hr exposure to 8.2 micrograms/liter. Mean blood glucose values were elevated after exposure to both endosulfan concentrations. The results are discussed in relation to the stress effect produced by the pesticide and the related responses of the fish.

Analysis of Variance↗
PubMed1995

[Ocular toxocariasis or retinoblastoma?].

BACKGROUND: Seroprevalence studies in Spain have shown that a high number of the population has been in contact with Toxocara canis although no cases of ocular involvement by this helminth have been described. The clinical and age of presentation makes differential diagnosis with retinoblastoma necessary, which is at time difficult, although essential since the treatment and prognosis are very different. METHODS: One case of ocular toxocariosis and the diagnostic keys based on the following are described: ophthalmologic exploration, imaging techniques (ECHO, TC, NMR), aqueous humor cytology, LDH quotient of aqueous/serum humor, and determination of specific anti-Toxocara antibodies by ELISA in serum and aqueous humor. CONCLUSIONS: Correct differential diagnosis between ocular toxocariosis and retinoblastoma is required.

Child, Preschool↗
PubMed1991

Gastric juice and analysis of basal and stimulated secretion following treatment with rice-bran oil.

In determining whether rice-bran oil (RBO) has antisecretory activity, we induced stress ulcers in 20 Wistar rats (10 controls; 10 animals given 0.2 ml/day RBO orally for 4 days prior to ulcer induction). In turn, we analyzed gastric juice for histamine, pepsin, H+ concentration ([H+]) and output volume. A second, complementary study was made of basal and stimulated gastric secretion through continuous "in vivo" recording of output. Secretion was stimulated with increasing doses of histamine, betanechol and pentagastrin. Ninety-four rats were used (47 controls and 47 rats given 0.2 ml/day RBO orally for 4 days prior to gastric output evaluation). The results were evaluated by the Student t-test. Ulcer index in the RBO-treated rats was significantly lower than among the controls (p less than 0.01), as also reflected by a significantly greater decrease in [H+] among the RBO-treated rats (p less than 0.05). However, no significant differences were observed for the remaining parameters. Continuous recording of basal gastric output showed a significant decrease in [H+] among the RBO-treated rats (p less than 0.01). Following histamine stimulation, [H+] was likewise significantly lower among the RBO-treated rats than in the controls. However, no significant differences were observed following stimulation with either betanechol or pentagastrin. RBO contains a high percentage of unsaturated fats; the latter act as precursors in the synthesis of arachidonic acid, which in turn is the essential precursor of prostaglandins--established inhibitors of gastric secretion. RBO also contains antioxidants such as alpha-tocopherol, which may likewise stimulate the synthesis of prostaglandins. RBO likely acts by increasing prostaglandin output, thus interfering with gastric HCl production.

Administration, Oral↗
PubMed1977

Neurosurgical infection treated with fosfomycin and 6-methylprednisolone.

A left parietal parasagittal meningioma was removed in a 67-year-old female patient. In the postoperative period she had a neurosurgical cerebral suppurating infection: subdural, epidural and epicranial, connected to the exterior by several fistulas. In the operative revision and after a culture, the germ causing the infection, E. coli, was isolated. Treatment was begun with 16 g of intravenous fosfomycin and 120 mg daily of 6-methylprednisolone intramuscularly, and this treatment cured the patient.

Aged↗
PubMedDate not supplied

Analysis of Fgf15 expression pattern in the mouse neural tube.

The dynamic process of neural tube regionalization in vertebrates is regulated by the expression of developmental genes which appear in characteristic patterns at neuroepithelial transversal domains, which are called secondary organizers. The molecular code present in these neuroepithelial organizers controls the generation of morphogenetic signals that induce and maintain regional characteristics in the surrounding neuroepithelium. The product of the Fgf8 gene is a secreted protein that has been demonstrated to be the key molecule for the isthmic organizer and is also expressed in two other organizer regions: the zona limitans and the anterior neural ridge. Here we analyze the expression of Fgf15 at different stages of mouse development in relation to Fgf8 and Otx2 expression patterns.

Animals↗