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Biomedical subjects

L Grillner

Publications and source records attributed to L Grillner.

At least 55 records · Page 3Linked to original sources
PubMed1990

Syphilis, gonorrhoea and genital chlamydial infection in a Somali village.

A total of 767 sera were collected from 187 men, 200 women and 380 children in a Somali village, Jambaluul. All sera were tested for syphilis serological markers by Venereal Diseases Research Laboratory (VDRL) and Treponema pallidum Haemagglutination Assay (TPHA). Sera positive for both or either of these tests were further analysed for the presence of specific IgM antibodies by Solid Phase Haemadsorption Assay (SPHA). A high and almost equal prevalence of TPHA positivity was found in men and women; 24% and 22.5%, respectively, and IgM antibodies were found in 3% and 4%, respectively. TPHA positivity significantly increased with age. Thus more than half of the villagers at the age of 45 years or more were TPHA positive. One percent of the children were TPHA positive. From all adults aged 15 years and above urogenital specimens were also taken for Chlamydia trachomatis antigen detection with an enzyme-amplified immunoassay (IDEIA) and Neisseria gonorrhoeae culture. Chlamydial genital infection was found in 6% of the men and 18% of the women. All gonococcal cultures were negative.

Adolescent↗
PubMed1990

Immunoglobulin contents in cervical secretions of women with chlamydial cervicitis.

Contents of albumin, IgG, IgA and secretory IgA (S-IgA) were determined in secretions of 16 women with chlamydial cervicitis (study group) and 13 women with vulvovaginal candidiasis (control group). The amounts of respective protein in cervical secretions were higher among the women with chlamydial infection representing both an increased transudation and an increased local production of antibodies in the infected cervix. Specific chlamydial antibodies of IgG, IgA and S-IgA class were found significantly more often in the study group. Comparing the IgG/IgA ratios in serum and secretions gave indications for an active local secretion of specific IgA antibodies. This was also supported by the demonstration of specific S-IgA.

Adult↗
PubMed1990

Sexually transmitted diseases in men in Mogadishu, Somalia.

The prevalence of viral and bacterial sexually transmitted diseases were studied in 101 men attending a dermatovenereal outpatient clinic in Mogadishu. A control group of 103 healthy adult men were included for the serological part of the study. Serological markers of hepatitis B virus (HBV), human immunodeficiency virus (HIV), cytomegalovirus (CMV) and herpes simplex virus (HSV) were studied. All sera were tested for syphilis markers. HBV serum markers were detected in 84% of the men in the study group and 66% of the healthy controls (P less than 0.005). Hepatitis B virus carriers were detected more frequently in the study group than among the controls. Also, 96% of the men in both groups had CMV antibodies and all of them had antibodies to HSV. No sera were found to contain HIV antibodies. The TPHA-positivity was 10% and 3% in the study and control groups respectively, and 5% of the patients had syphilis IgM antibodies. Sexual contact with prostitutes was recorded in 54% and 48% respectively of patients and controls, and such contact was correlated with TPHA-positivity in the study group. Chlamydia trachomatis antigen was detected in urogenital specimens of 14% of the men in the study group and gonococcal culture was positive in 53% of those with urethral discharge.

Adolescent↗
PubMed1990

Antibodies against hepatitis C in a population of Swedish haemophiliacs and heterosexual partners.

The prevalence of antibodies against hepatitis C virus in 160 Swedish patients with haemophilia or related coagulation disorders and 13 heterosexual partners was assessed by means of an ELISA method. A high prevalence of HCV antibodies was found in haemophiliacs with chronic hepatitis (87%), with HIV-antibodies (87%), with severe form of the coagulopathy (81%), and among those who at any point had received factor concentrates without viral inactivation (79%) or of foreign origin (84%). On the other hand, none of the partners were seropositive, indicating a low risk of sexual transmission.

Adolescent↗
PubMed1990

Aetiology, outcome and prognostic factors in community-acquired pneumonia requiring hospitalization.

Outcome and prognostic factors were prospectively studied in 277 adult patients (average age 62 yrs) with community-acquired pneumonia requiring hospitalization. The aetiology was established in 68%, with S. pneumoniae as the predominating agent. Mortality was 4% (12 of 277), and all but one who died were greater than or equal to 60 yrs of age. Features associated with high mortality included greater age, absence of chills and chest pain, high respiratory rate (greater than 30 breaths.min-1) and low serum albumin on admission, and the occurrence of airway colonization and secondary infection during hospital stay. Multivariate analysis showed that low serum albumin and the occurrence of secondary infection, but also absence of chills and airway colonization, were correlated to a higher mortality. In patients who survived, the median length of hospital stay was seven days, and at follow-up, about eight weeks after admission, 81% had recovered and chest X-ray was normal in 84%. In conclusion, we believe that the outcome of community-acquired pneumonia can be influenced by prophylactic measures against pneumococcal infection, and an increased surveillance of risk patients.

Adolescent↗
PubMed1989

Characterization of two genome types of adenovirus type 31 isolated in Stockholm during 1987.

The authors isolated during 1987 seven adenovirus type 31 (Ad31) within a 9-month period. The isolates were obtained from urine, throat, and feces, implying a systemic spread of the infection. Most patients displayed gastrointestinal symptoms, but some had respiratory symptoms and fever. All of the strains differed by restriction endonuclease analysis from the prototype strain (1315) by an additional Bgl II restriction site. Ad31 isolates 1-6 could be divided into two groups by the enzymes Bam HI, Msp I, and Xho I. Each enzyme gave rise to the same group distribution: isolates 1-3 and 4-6, respectively. Digestion with Bst EII, Hind III, Kpn I, and Sma I resulted in identical patterns for isolates 1-6. Isolate 7, however, demonstrated a DNA deletion of approximately 0.8 kbp, but it was otherwise identical to isolates 4-6. In conclusion, two separate genome types of Ad31 were isolated, one of which included a DNA deletion mutant. The increased isolation rate may reflect an epidemiological situation, as the same isolation procedure had been used both before and after this period.

Adenovirus Infections, Human↗
PubMed1988

Transfusion transmitted human T-lymphotropic virus infections.

Human immunodeficiency virus (HIV) type 1 has emerged during the 1980s as an important transfusion transmitted agent. The impact of HIV depends on the epidemiology and characteristics of the virus, serological response to infection and efficacy of serological tests to identify infected blood units. These factors will be described in the present article. The risk to acquire HIV today, by transfusion of anti-HIV screened blood, is extremely small in Scandinavia and the Western world. The risk can be further minimized by more sensitive screening tests and an improved donor information. The epidemiology in Scandinavia of other members of the human T-lymphotrophic viruses, HTLV-1 and HIV-2, has to be further investigated.

Acquired Immunodeficiency Syndrome↗
PubMed1988

Detection of cytomegalovirus by in situ hybridisation and immunohistochemistry using new monoclonal antibody CCH2: a comparison of methods.

In situ hybridisation, immunohistochemistry, and morphological analysis for the detection of cytomegalovirus (CMV) were compared in routinely processed tissue sections from a patient with acquired immune deficiency syndrome (AIDS) and widespread CMV infection. Both in situ hybridisation and immunohistochemistry with the monoclonal antibody CCH2 labelled all "owl's eye" cells intensely and, in addition, nuclei of some morphologically normal cells. Quantitative evaluation of the results showed that in situ hybridisation and immunohistochemistry with CCH2 were considerably more sensitive than purely morphological analysis, particularly in tissues with only a few cells infected by CMV. It is further shown that immunohistochemistry with CCH2 detected a higher figure of CMV infected cells than in situ hybridisation. In conclusion, both in situ hybridisation and immunohistochemistry are rapid, sensitive, and specific methods for CMV detection. For routine purposes, however, immunohistochemistry seems to be more suitable.

Acquired Immunodeficiency Syndrome↗
PubMed1988

Endonuclease cleavage pattern of cytomegalovirus DNA of strains isolated from congenitally infected infants with neurologic sequelae.

A prospective study of congenital cytomegalovirus infection in Malmö, Sweden, was performed from 1977 through 1985. The diagnosis was based on isolation of cytomegalovirus soon after birth. Congenital cytomegalovirus infection was identified in 76 infants, and as of September 1986 CNS symptoms have been experienced by nine of them. In at least seven of these infants, the disturbances can be referred to the cytomegalovirus infection. The strains from eight of the nine infants have been further studied by restriction endonuclease analysis of cytomegalovirus DNA. The cleavage patterns obtained with BamHI, EcoRI, and HindIII showed a unique pattern for each one of the eight strains. No common pattern could be associated with these eight strains in comparison with strains from postnatally infected children.

Child↗
PubMed1988

HIV transmission by blood transfusions in Stockholm 1979-1985: nearly uniform transmission from infected donors.

Thirty-five out of approximately 800 known HIV-seropositive people in Stockholm by mid-1986 were blood donors during the period 1979-1986. Almost all, i.e. 349 recipients of their blood components (red blood cells, platelets, plasma) could be traced. One hundred and eighty were still alive and 112 of these on further analysis, were suspected of being infected. They were contacted and all but one agreed to be tested for HIV antibodies. Fifty recipients were found to be seropositive. They had been transfused with blood components from 14 of the 35 donors. The earliest observed transmission occurred in June 1982. The patterns of HIV transmission showed, with only one exception, that each donor who had transmitted HIV to one recipient had also transmitted it to all later recipients. Appropriate preserved sera and clinical records from five of the donors who had not transmitted the virus were found and analysed. The result indicated that these donors had acquired their HIV infection after their last blood donation. In conclusion, our study indicates that every antibody-positive donor transmits HIV to almost every recipient.

Acquired Immunodeficiency Syndrome↗
PubMed1987

Screening of blood donors for cytomegalovirus (CMV) antibodies: an evaluation of different tests.

The incidence of transfusion-associated cytomegalovirus (CMV) infections is related to the number of donors and the volume of blood. In immunosuppressed patients primary CMV infection is associated with a high morbidity and/or mortality. These infections can be prevented by the use of CMV negative blood products. A test for screening of CMV antibodies should have a high sensitivity and specificity and in addition be rapid and easily automated. In the present study the routine method in the laboratory, a CMV ELISA using a nuclear CMV antigen, was compared with Vironostika CMV anti-IgG MicroELISA, CMVSCAN, a latex agglutination test and a new ELISA from Organon Teknika, Vironostika CMV one-step MicroELISA. Sera from 419 blood donors were tested by all four tests. Discordant results were resolved by Western blot analysis using a glycine-extracted CMV antigen. The sensitivity was 100% for CMV ELISA and CMVSCAN and 99 and 96% for the CMV anti-IgG and CMV one-step tests respectively. The specificity was 100, 99, 94 and 100%, respectively. The ELISAs are suitable for screening of large numbers of serum samples and the CMV one-step test in particular was rapid and easy to perform although the sensitivity has to be increased. The latex agglutination test has the advantage of being extremely rapid.

Antibodies, Viral↗
PubMed1987

Genomic comparison of herpes simplex virus type 1 isolates from Japan, Sweden and Kenya.

One-hundred and seventy-two epidemiologically unrelated herpes simplex virus type 1 (HSV-1) strains isolated in Japan (104 strains) and Sweden (68 strains) were compared by analysis of their genome structures using four restriction endonucleases, BamHI, KpnI, SalI and HindIII. In addition, 32 Kenyan HSV-1 isolates previously compared to Japanese isolates were included for further comparison with the Swedish isolates. Remarkable and statistically significant differences were found between the HSV-1 isolates from the three countries. One-hundred and thirty cleavage sites were examined, and it was shown that isolates from two of the three countries were statistically distinguishable at 27 of these loci. Pairwise comparison between isolates from Japan and Sweden, Kenya and Sweden, and Japan and Kenya revealed variation in 18, 16 and 23 sites, respectively. By considering gains and losses of 19 sites, the total of 204 strains could be classified into 92 distinct cleavage patterns. Isolates from the three countries could be distinguished from each other by the pattern, except for one Swedish and two Kenyan isolates which shared a pattern. Twenty-one fragments that were present or absent only in individual isolates from one or other of the three countries could be detected. These results show that HSV-1 strains from geographically separate countries or anthropologically different races have distinct distributions of endonuclease recognition sites.

DNA Restriction Enzymes↗
PubMed1987

Genome variations in herpes simplex virus type 2 strains isolated in Japan and Sweden.

One-hundred and twenty-three epidemiologically unrelated strains of herpes simplex virus type 2 (HSV-2) isolated in Japan and Sweden (68 Japanese and 55 Swedish isolates) were compared by analysis of their genomes using five restriction endonucleases: BamHI, KpnI, EcoRI, HindIII and Bg/II. Seven of the 93 restriction sites examined showed statistically significant variation between isolates from the two countries. However, HSV-2 isolates were less variable than the HSV-1 isolates previously analysed from the same countries. Using 12 restriction sites as markers, the HSV-2 isolates were classified into 41 cleavage patterns; 17 were specific for Japanese isolates and 15 were specific for Swedish isolates. Correlation coefficients between some sets of 12 markers were significant, but significant correlations between Japanese and Swedish isolates were distinct for each country. Both Japanese and Swedish isolates were assigned to three major patterns with no significant difference in incidence. In contrast, in two other major patterns, differences in incidence between the isolates were statistically significant. These results suggest that HSV-2 populations in geographically separated countries have distinct cleavage site distributions.

DNA Restriction Enzymes↗
PubMed1987

Sexually transmitted viral infections in various population groups in Mogadishu, Somalia.

The prevalence of serum antibodies to human immunodeficiency virus (HIV), herpes simplex virus (HSV), and cytomegalovirus (CMV) and of hepatitis B virus (HBV) markers was investigated in different population groups, including prostitutes, in Mogadishu, Somalia. Hepatitis B surface antigen (HBsAg) was detected in 37% of pregnant women, 4% of neonates, 22% of educated women, and 20% of prostitutes. No significant difference between the groups was observed for HBV. In contrast to figures reported from South East Asia, the prevalence of hepatitis Be antigen (HBeAg) was 18% in prostitutes and only 3% in all other HBsAg positive subjects. The prevalence of antibodies to HSV (100%) and CMV (90%) was very high, but antibodies against HIV were not detected in any of 471 sera. As the routes of transmission for HBV and HIV infections are considered to be similar, HIV will probably spread rapidly in Somalia once this virus has been introduced into the country.

Acquired Immunodeficiency Syndrome↗