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L Grimmonprez

Publications and source records attributed to L Grimmonprez.

13 recordsLinked to original sources
PubMed1997

Chemical characterization of sialyl oligosaccharides isolated from goat (Capra hircus) milk.

Four sialyl oligosaccharides were separated from goat milk (Capra hircus) by gel filtration and ion exchange chromatography. Their monosaccharide compositions determined by GC analysis after methanolysis and trifluoroacetylation were Glc (1) Gal (1) Neu5Ac (1) and Glc (1) Gal (2) Neu5Ac (1). Their chemical structures were further elucidated by methylation, FAB-MS and 1H-NMR analysis. The results suggest that their structures are: [structures: see text].

Animals↗
PubMed1990

Variations in oligosaccharides and lactose in human milk during the first week of lactation.

Variations in oligosaccharides and lactose in human milk were studied in 15 mothers during the first week of lactation. The neuraminyloligosaccharides and heavy neutral oligosaccharides increased slightly from days 2 to 5 postpartum and appeared to decrease until day 7. The lacto-N-difucohexaoses, lacto-N-fucopentaoses, and lacto-N-tetraose increased until day 5 and then decreased. Lactodifucotetraose and the fucosidolactoses decreased substantially until day 5 (p less than 0.05) and appeared to stablize in the following days. Lactose increased until day 5 (p less than 0.05) and continued to increase thereafter. Lactose was negatively correlated with total oligosaccharides (p less than 0.10). The fluctuations observed in total oligosaccharides from days 2 to 5 postpartum and their subsequent stabilization and regular decrease during lactation confirm the hypothesis of Kulski and Hartmann that mammary secretion occurs in three periods: colostrum for the first 36 h postpartum, transitional milk from days 2 to 5 postpartum, and mature milk after day 5. The oligosaccharide variations we found corresponded to those of other milk constituents observed by other authors. The significance of the oligosaccharide variations is discussed.

Adult↗
PubMed1990

Discriminant carbohydrate components of human milk according to donor secretor types.

Because of the variability of human milk carbohydrate composition, we determined the discriminant carbohydrate components of the milk of 18 mothers according to their ABH and Lewis secretor types during the first week of lactation. Comparative chromatograms revealed that the presence of neuraminyloligosaccharides is linked to the ABH secretor groups, and that the absence of oligosaccharides with Lea or Leb specificity is linked to the Lewis nonsecretor types. The study of carbohydrate composition according to donor secretor types consisted of measuring 16 variables from 69 samples. Analysis of variance showed significant differences between groups: high levels of N-acetylneuraminic acid and low levels of galactose distinguished ABH secretors from nonsecretors (p less than 0.001). In the ABH secretor groups, A and H secretors had higher N-acetylglucosamine contents than B and AB secretors (p less than 0.001) and lower galactose levels (p less than 0.001). The Lewis secretor groups were distinguished by significantly higher fucose levels (p less than 0.001). The ABH(+)Le(a-b-) group had higher lactose contents than the other groups (p less than 0.01).

ABO Blood-Group System↗
PubMed1986

Diurnal variations and within the feed in lactose and oligosaccharides of human milk.

Variations of lactose and oligosaccharides in human milk were studied during the day and the feed, by dialysis, chromatography on Bio-gel P-2 column, paper chromatography, colorimetric analysis and gas chromatography. Progressive changes were found during the day, but not during the feed. A diurnal variation in milk lactose was seen, which was the inverse of the oligosaccharides. Significant negative correlations between lactose and oligosaccharides were seen in the afternoon and evening (p less than 0.05). The minimal mean value of lactose at 19.00 h is 58.64 +/- 5.28 g/l and the maximal mean values of oligosaccharides was 17.93 +/- 2.28 g/l. The data are discussed with regard to other milk constituents and to their possible physiological relevance.

Acetylglucosamine↗
PubMed1985

Variations of lactose and oligosaccharides in milk from women of blood types secretor A or H, secretor Lewis, and secretor H/nonsecretor Lewis during the course of lactation.

Variations of lactose and oligosaccharides in human milk were studied over a 3-month lactation period by dialysis, chromatography on Bio-gel P-2 column, paper chromatography, colorimetric analysis and gas chromatography. Milk samples were collected from donors of various blood and secretor types. An increase in the concentration of lactose was noted: 59.25 +/- 1.61 to 72.17 +/- 1.35 g/l for the group of secretor A secretor Lea Leb individuals, and from 62.25 +/- 1.35 to 73.15 +/- 3.45 g/l for the group of secretor H secretor Lea Leb individuals. A decrease in the concentration of oligosaccharides was also found: from 16.71 +/- 0.99 to 7.90 +/- 1.29 g/l and from 18.51 +/- 0.74 to 7.33 +/- 0.65 g/l, respectively, for these same groups. In secretor H nonsecretor Lewis individuals, the concentration of lactose increased from 67.97 +/- 3.09 to 77.42 +/- 1.88 g/l, and the concentration of oligosaccharides decreased from 13.27 +/- 0.40 to 3.47 +/- 0.33 g/l. Analysis of oligosaccharide composition by chromatography showed that variations are in relation to the secretor Lewis type. All oligosaccharides decreased equally throughout the lactation period, regardless of the blood group. The decrease was more rapid, however, in the nonsecretor Lewis individuals. In these individuals, the absence of certain oligosaccharides is not compensated by an overproduction of the other oligosaccharides present or by biosynthesis of structurally different oligosaccharides. The origin of these oligosaccharides is discussed.

ABO Blood-Group System↗
PubMed1975

[Isolation and physico-chemical properties of oligosaccharides of human milk].

From a non-dialysable fraction of human milk the authors have isolated 30 oligosaccharides by combining anion exchange and paper chromatography. These oligosaccharides (MW 998 to 3113) contain D (+)-galactose, L (-)-fucose, N-acetyl-D (+)-glucosamine, N-acetyl-D (-)-neuraminic acid in variable proportions and one single D (+)-glucose residue in terminal reducing position. The characteristics of the oligosaccharides are those of the so-called "Polonovski and Lespagnol gynolactose". These sugars do not originate from glycoproteins nor glycolipids. The authors suggest that the biosynthesis of human milk oligosaccharides is due to an activation of glycosyltransferases related to blood group substances, induced by lactose. They base this hypothesis on the fact that, as demonstrated by Strecker and Montreuil, spontaneous or glucose, galactose and lactose induced meliturias are accompagnied by an important urinary excretion of blood group substance related oligosaccharides.

Acetylglucosamine↗