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Biomedical subjects

L H Ostergaard

Publications and source records attributed to L H Ostergaard.

2 recordsLinked to original sources

[Polyketides--status and perspective].

The increased resistance among pathogenic bacteria is an important reason for research into new drugs and into improved efficiency of the ones already existing. A group of natural products, the polyketides, exhibits a range of antibiotic and other important pharmacological activities. This group of natural products has therefore attracted much attention from researchers world-wide. The biosynthesis of polyketides occurs in a stepwise fashion on gigantic multienzymes. Individual enzymatic entities within these multienzymes are responsible for catalysis of each step in the biosynthetic pathway of the polyketide chain. It has been demonstrated that there is a direct correlation between the DNA-sequence encoding these enzymatic units and the order in which the reactions are catalysed. Polyketide synthases may thus be considered a molecular assembly line, and new polyketides can be designed by changing the modules of the assembly line. An understanding of the rules governing the genetic manipulation of the synthases is of course of the utmost importance for success. The first major steps towards this goal have been taken with the results from a number of new experiments.

Anti-Bacterial Agents↗

Development and comparison of purification strategies for chicken antibodies from egg yolk.

With regard for animal welfare as well as cost, antibodies purified from the egg yolk of immunized chickens are an attractive alternative to antibodies raised in serum of mammals. Various purification strategies based on initial purification by a freeze-and-thaw method followed by one of three precipitation methods--ammonium sulfate, polyethylene glycol 6000, or caprylic acid--were compared. Ammonium sulfate precipitation (25 to 40%) was found to give the best combination of high purity and yield (58%). Moreover, this method is inexpensive and well suited for standard purification of egg yolk antibodies in large scale production facilities.

Ammonium Sulfate↗