[A 3H-thymidine paper strip method for stimulation of lymphocytes. I. Culture conditions for human or murine lymphocytes and reproducibility of the paper strip method].
A simple method for stimulation of human or murine lymphocytes is described. As a possible more rapid test than the standard estimation of [3H]-thymidine uptake after solubilisation of cell sediments, paper strip counting in a liquid scintillation counter was studied in 80 individuals and 20 mice. Counting efficiency approaches 1%, the reproducibility is good within the acceptable 16% range.
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