PubMed Health⌕ Search

Biomedical subjects

L Hummel

Publications and source records attributed to L Hummel.

At least 19 recordsLinked to original sources
PubMed2002

Introgression of a quantitative trait locus for yield from Glycine soja into commercial soybean cultivars.

The value of exotic germplasm in broadening the genetic base of most crops has been demonstrated many times. However, the difficulties involved in working with exotic germplasm have limited their utility in plant breeding. Unwanted linkages often thwart the successful incorporation of beneficial exotic genes into commercial lines. Thus, the use of exotics in traditional breeding makes the process of crop improvement a tedious, time-consuming and expensive endeavor. The availability of molecular markers makes it possible to isolate specific genomic regions and transfer them into commercial varieties with minimal linkage drag. We found a yield-enhancing quantitative trait locus (QTL) from Glycine soja (Siebold and Zucc.) by evaluating a population of 265 BC(2) individuals from a cross between HS-1 and PI 407305. The yield QTL was located on linkage group B2(U26) of the soybean [Glycine max (L.) Merrill] genetic linkage map. In a 2-year, multi-location study, individuals carrying the PI 407305 haplotype at the QTL locus demonstrated a 9.4% yield advantage over individuals that did not contain the exotic haplotype. When tested in a more uniform "HS-1-like" background in two locations, we observed an 8% yield advantage for lines that carry the PI 407305 haplotype. We further assessed the QTL effect in various elite soybean genetic backgrounds. The yield effect was consistently observed in only two of six genetic backgrounds. Individuals carrying the PI 407305 haplotype at the QTL locus had a 9% yield advantage in yield trials across locations. Despite the limited adaptability of this yield-QTL across genetic backgrounds, this study demonstrates the potential of exotic germplasm for yield enhancement in soybean.

Alleles↗
PubMed1996

[Blood collection from central venous catheter--valid also for blood coagulation analysis?].

OBJECTIVE: Blood samples obtained from centralvenous catheters are commonly used on intensive care units. In this paper the question of valid conditions for sampling blood coagulation parameters of patients with heparinised catheters is examined and the data are compared with the literature. STUDY DESIGN: Blood samples were obtained at the same time as well via centralvenous catheters as from the periphery. The results of the examination were compared to each other. RESULTS: After the aspiration of 10 ml of blood via centralvenous catheters there were no differences between peripheral and central obtained samples as regards blood coagulation parameters.

Adult↗
PubMed1995

[Blood specimen collection from central venous catheters--reliable also for blood coagulation analysis?].

Blood probes obtained from the central-venous catheter are common methods on intensive care units. In the present paper, the conditions for obtaining blood samples for clotting analysis of patients with heparinized catheters are examined and compared to the literature. In these patients, blood samples were drawn simultaneously from central as well as peripher lines. The results of the examination were prospectively compared. After the aspiration of 10 ml blood from centralvenous catheters, there are no differences between peripher and central venous blood samples.

Adult↗
PubMed1993

[Importance of the fibrinogen concentration as an independent risk factor for ischemic heart disease].

Today, the determination of plasma fibrinogen levels is not very helpful in the identification of individuals at risk for myocardial infarction. This is due to the fact that there is a significant overlap between the fibrinogen levels of individuals at risk and controls. Until now, the methodological problems of fibrinogen measurement are not fully solved. On an individual basis, the prediction of the risk for myocardial infarction from the level of fibrinogen in association with other risk factors is poor because of the absence of an appropriate diagnostic model.

Adult↗
PubMed1992

[A problematic case of dysfibrinogenemia in gynecology].

Following an hymenal incision in a thirtheen years old patient a haemostaseological examination was done. A hyperfibrinolysis was supposed. In spite of a good clinical recovery values remained pathological, so we had to suspect a dysfibrinogenaemie. Only a rapid examination of all attainable family members was able to secure the diagnosis: Dysfibrinogenaemie. Dysfibrinogenaemia is not a to rare case, that such a diagnostic problem may arise in every department.

Adolescent↗
PubMed1989

Rates of de novo fatty acid synthesis in liver, muscle and adipose tissue in non-pregnant and pregnant rats in vivo.

Fatty acid de novo synthesis was measured quantitatively in liver, muscle and adipose tissue of non-pregnant and 21-day pregnant Uje: WIST rats in vivo. By means of 3H2O incorporation experiments the following rates of fatty acid synthesis, expressed as mumol palmitate-equivalents/animal.min, were calculated in non-pregnant and pregnant animals: liver 0.20 +/- 0.06 and 0.24 +/- 0.06; muscle 0.21 +/- 0.06 and 0.24 +/- 0.08; adipose tissue, 0.36 +/- 0.10 and 0.28 +/- 0.09, respectively. Thus, liver, muscle and adipose tissue contribute at approximately equal amounts to the summarized rate of fatty acid synthesis, which is similar in both pregnant and non-pregnant rats.

Adipose Tissue↗
PubMed1988

Problems of the laboratory control of oral anticoagulant treatment.

The paper deals with the estimation (using the Monte Carlo method) of a confidence interval for an individual patient's International Normalized Ratio (INR) of 3.3. The results show that this confidence interval ranges from an INR of 2.5 to 4.2 and is of about the same size as the therapeutic range.

Administration, Oral↗
PubMed1987

[Blood clot observation test].

In the literature the blood clotting time determined by the clot observation test ranges from 8 min to 20 min; apparently, this wide range is due to the different conditions used. Therefore, under similar conditions a comparative measurement of the clotting time of a control blood is proposed in order to make an assessment of hypocoagulability possible.

Blood Coagulation Disorders↗
PubMed1986

Quantitative studies on the fetal lipid metabolism in rats: liver fatty acid esterification and conversion into carbon dioxide, and hepatic output of triglycerides and phospholipids into serum.

Fetal lipid metabolism was studied quantitatively using the 14C-1-palmitate in vivo tracer technique and compartment analysis, and in vitro incorporation experiments. The following conclusions can be drawn from our experiments. Fetal serum free fatty acids (FFA) exchange so rapidly with the FFA of another compartment that the FFA of both compartments can be considered as one homogeneous FFA compartment. The turnover time of this compartment was calculated to be 0.76 mumol FFA/min/l through this compartment. The incorporation of fetal serum FFAs into fetal liver triglyceride fatty acids (TG-FA) and phospholipid fatty acids (PL-FA) was calculated to be 0.05 and 0.14 mumol FFA/min/l, respectively. Thus, only 16% of the fetal serum FFA is incorporated into fetal liver lipids. The fetal serum TG-FA most likely originate in the fetal liver. The fetal liver puts out 0.025 mumol TG-FA/min/l and 0.10 mumol PL-FA/min/l into the fetal serum. The turnover times of fetal serum TG-FA and PL-FA are 100 and 39 min, respectively. The fetal liver conversion rate of fatty acids (FA) into CO2 determined in vitro (0.06 mumol FA/min/l) amounts to about 25% of the rate seen in the whole rat fetus.

Animals↗
PubMed1984

Studies on the fatty acid synthesis of the perfused cirrhotic liver.

The fatty acid synthesis rate (using 3H2O incorporation experiments) and bile composition were studied in thioacetamide induced cirrhotic rat livers. The results show that the fatty acid synthesis rate of cirrhotic livers expressed as mumoles newly synthesized fatty acids per g liver is smaller than that of controls. Transient changes were observed in the incorporation pattern of newly synthesized fatty acids into individual lipid classes between cirrhotic and control livers. The output of bile acids is significantly decreased in cirrhotic livers.

Animals↗
PubMed1983

Maternal-fetal transfer of free fatty acids during late gestation in the rat.

The maternal-fetal transfer of free fatty acids during late gestation in the rat has been investigated. The isotopic tracer technique and a mathematical model were used. From the results the conclusion can be drawn that the fetus from day 15 to 19 of pregnancy derives only 10% of the fetal fatty acids from the maternal circulation.

Animals↗
PubMed1982

Glucagon stimulation of hepatic Na+, K+-ATPase.

In the perfused rat liver administration of glucagon was shown to result in a transiently increased uptake of K+, indicating the possible involvement of the Na+, K+-ATPase. Direct measurement of the activity of Na+, K+-ATPase revealed a two-fold stimulation of the enzyme by glucagon. The effect of glucagon on the activity of the enzyme was immediate. Simultaneously with the increase in the activity of the Na+, K+-ATPase, the activity of Mg2+-ATPase decreased. In order to evaluate whether the activation of the Na+, K+-ATPase by glucagon is related to the metabolic effects of the hormone, experimental conditions known to interfere with the activity of the enzyme were employed and glucagon stimulation of Ca2+-efflux, mitochondrial metabolism and gluconeogenesis were measured. K+-free perfusate, high K+ perfusate or ouabain interfered to varying degrees with the glucagon stimulation of these responses. The combination of K+-free perfusate and ouabain almost completely abolished the glucagon stimulation of all three parameters. These results demonstrate the glucagon stimulation of Na+, K+-ATPase and raise the possibility that the activation of the enzyme by glucagon might be a necessary link for the manifestation of its metabolic effects.

Animals↗
PubMed1982

Studies on the hypertriglyceridemia in pregnant rats.

The study has been performed in order to obtain information on the mechanism responsible for the hypertriglyceridemia found in pregnant rats. The results show that the whole plasma triglyceride flux is increased by 65% in late gestation. It has been found that labeled VLDL triglyceride originating from pregnant rats shows no delay in plasma disappearance when injected into the circulation of non pregnant female rats. Contrariwise, labeled VLDL triglyceride originating from non pregnant rats when injected into pregnant rats shows a delay in disappearance from the circulation. From these results the conclusion can be drawn that the delay in labeled plasma triglyceride disappearance in late gestation is not caused by a possibly abnormal VLDL showing a decrease in affinity of lipoprotein substrate for lipoprotein lipase. Therefore, we interpret the delay in labeled plasma triglyceride disappearance in late gestation in terms of a lowered removal capacity of plasma triglyceride by extrahepatic tissue.

Animals↗