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Biomedical subjects

L Hurst

Publications and source records attributed to L Hurst.

At least 19 recordsLinked to original sources
PubMed1992

Herpes simplex virus type 1 infection of mouse astrocytes treated with basic fibroblast growth factor.

We explored a possible role for the basic fibroblast growth factor (FGF) receptor during herpes simplex virus type 1 (HSV-1) infection of primary mouse astrocytes, glial cells of the central nervous system known to express FGF receptors. Plaque reduction experiments showed that treatment of astrocyte monolayers with human recombinant basic FGF failed to inhibit HSV-1 infectivity, although treatment with either heparin or poly-L-lysine reduced it by approximately 100%. Identical results were obtained when monolayers of human embryonic lung fibroblasts or African green monkey kidney cells were treated with FGF, heparin or poly-L-lysine prior to HSV-1 infection. We conclude that the basic FGF receptor is not involved in the uptake of HSV-1 during productive infection of astrocytes. Our findings suggest that this molecule is not the predominant cellular receptor for HSV-1 among vertebrate cells and plays no role in defining HSV-1 neurotropism in vivo.

Animals↗
PubMed1986

Material prepared from normal brain inhibits migration of leukocytes from Huntington's disease patients.

Material inhibiting migration of leukocytes in vitro from Huntington's disease patients, was obtained from human brain removed at autopsy. The active material was present in brainstem, basal ganglia and cerebellum but not in cerebral cortex. It could be recovered at autopsy up to 21.5 h after death, but not from patients who had died following prolonged anoxia. It was susceptible to heating and to freezing and thawing. Its activity was preserved in acid medium to pH 3, and its iso-electric point was around pH 5. Activity was present in the fraction eluted from gel filtration with molecular weight of 1.9 X 10(5). Protein, carbohydrate, lipid and nucleic acid could be identified in the active material, and its activity depended on intact protein and carbohydrate moieties. Sialic acid, although present, did not contribute to antigenicity. Extraction with lipid solvents abolished activity.

Aged↗
PubMed1984

Effects of low-modulus coatings on pin-bone contact stresses in external fixation.

The intent of this study was to investigate the stress distribution in cortical bone around fracture fixation pins and around pins coated with various polymeric and elastomeric materials. Since these interface stresses cannot be measured directly, a photoelastic technique was employed and stresses were measured in two-dimensional bone models fabricated from sheets of epoxy resin. Our results showed that when a fixation pin was loaded in compression, the compressive stress measured in the model was greatest at the pin-model interface. The magnitude of the compressive stress was found to diminish steeply away from the hole in a log decrement distribution which was asymptotic to the value of the average stress in the model. When polymeric and elastomeric materials were applied as pin coatings and the performance of the coated pins was compared to that of uncoated pins of the same overall diameter, a reduction of the maximum stress in the bone model was demonstrated. Among the coatings tested, we found that of the polymeric materials ultrahigh molecular weight polyethylene (UHMWPE) was most effective at reducing the peak cortical stress magnitude. The most effective coating material overall was found to be silicon elastomer. Computation of stress values in models loaded through stainless-steel pins and through pins coated with 1-mm silicon elastomer showed that the presence of the elastomer layer caused a reduction of about 50% in the maximum compressive stress in the model.

Biomechanical Phenomena↗
PubMed1981

An unusual first branchial cleft cyst.

First branchial cleft anomalies have been documented with increasing frequency within the past decade, with more than 200 cases reported to date. A case of a first branchial cleft cyst with some unusual features is presented. The literature of such anomalies is reviewed, with some distinct clinical differences being noted between cysts and other branchial cleft anomalies.

Adult↗