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Biomedical subjects

L Hutton

Publications and source records attributed to L Hutton.

At least 19 recordsLinked to original sources
PubMed1993

[Right diaphragmatic paralysis after liver transplantation].

Right hemidiaphragm paralysis has been previously documented in patients after orthotopic liver transplantation (OLT) and it may contribute to the development of postoperative pulmonary problems. It has been postulated that a crush injury to the right phrenic nerve during OLT is the cause of dysfunction of the right hemidiaphragm. To assess the incidence and effect of right phrenic nerve injury after OLT, we prospectively studied 51 adult liver recipients that we compared with twelve patients who underwent liver resection (LR) without suprahepatic vena cava clamping. We studied the diaphragm excursion by ultrasound, the pulmonary function tests and the transcutaneous phrenic nerve conduction. Righ phrenic nerve injury and hemidiaphragm paralysis occurred respectively in 79% and 38% of the liver recipients but not after LR. Conduction along the right phrenic nerve was absent in 53% of the patients and reduced in another 26%. Left phrenic nerve conduction and left hemidiaphragm excursion were normal in both groups. Liver recipients with no conduction in the right phrenic nerve had a significantly greater decrease in vital capacity in the supine position compared to those with some conduction (29% vs 14%; P < 0.001). However, neither the time on the ventilator or the hospital stay were significantly different between the two groups. Complete recovery of phrenic nerve conduction and diaphragm function may take up to nine months. Right phrenic nerve injury is common after OLT and causes right hemidiaphragm dysfunction.

Adolescent↗
PubMed1990

Inhaled particle deposition and body habitus.

As a result of the intrapleural pressure gradient that exists in the human lung, both ventilation and particle deposition increase from apex to base. Since the intrapleural gradient varies with the height of the subject, it was decided to compare regional particle deposition in tall, short, and obese subjects to ascertain whether it was influenced by height and weight. Surprisingly, deposition in the vertical plane was not significantly influenced by the height of the subject when corrected for ventilated lung volume. In addition, it was shown that in obese subjects there was increased deposition in the middle zones relative to the apices and bases. This finding persisted after correction for ventilated lung volume and differential attenuation resulting from non-uniform thickness of the fat layer in the obese subject's chest. In the tall and short groups there was a consistent pattern in the concentric deposition of particles with there being a gradient from the central or hilar region to the periphery of the lungs, with the latter showing the most deposition.

Adipose Tissue↗
PubMed1987

The P55 protein affected by v-mos expression is vimentin.

Rabbit antiserum prepared against a cyclic 19-amino-acid peptide predicted from the sequence of the viral mos gene (v-mos) of Moloney murine sarcoma virus not only recognized v-mos gene products but also specifically detected a 55,000-Mr polypeptide expressed in a variety of cells that grow on solid surfaces. This normal cellular protein, previously shown to be reduced in amount in cells expressing the v-mos gene, was found to be the intermediate filament structural protein, vimentin. This conclusion was reached by comparing relative mobilities in denaturing gels, isoelectric points, immunoreactivities, location in the cell, and peptide maps.

Animals↗
PubMed1986

Renografin-60 for urography: effect on serum electrolytes and proteins in adults.

A prospective study was done on 27 adults to assess the changes in serum electrolytes and proteins induced by bolus administration of 100 ml of the ionic contrast medium diatrizoate meglumine and sodium (Renografin-60) for IV urography. Statistically significant changes in serum sodium, chloride, potassium, calcium, bicarbonate, phosphate, total proteins, and albumin were shown at 5 min postinjection. The mean percentage decreases were sodium 2%, chloride 2%, potassium 9%, calcium 13%, bicarbonate 9%, phosphate 10%, and proteins 15%. A mean 3% increase in serum osmolality was observed. By 30 min, sodium and chloride levels had returned to baseline; potassium, calcium, and albumin values were incompletely recovered; bicarbonate was not significantly changed from 5 min; and phosphate values continued to decrease. An in vitro dialysis experiment in which different volumes of Renografin-60 were dialysed against an electrolyte solution (pseudoserum) produced a dilutional factor of 5 to render a given volume of the contrast isoosmotic with plasma. The observed changes from the baseline values of the electrolytes and proteins up to 10% are therefore assumed to be due to hemodilution resulting from movement of fluid from the extravascular to the intravascular compartment. This study confirms alterations in serum levels of several electrolytes after the use of ionic contrast media beyond simple hemodilution. Although these changes appear not to be clinically significant in this investigation, the alterations in potassium and calcium may contribute to arrhythmias, particularly when hypokalemia or hypocalcemia preexists.

Adolescent↗
PubMed1986

Radiologic findings of retroperitoneal perforation after sphincterotomy.

The radiographs of all patients with perforation into the retroperitoneum following endoscopic sphincterotomy, performed between 1981 to 1984 at University Hospital, London, Ontario were reviewed retrospectively. The incidence of perforation was very low. There were only three patients with perforation (1.1%), although 264 sphincterotomies had been performed. The initial and most important radiological findings are air and contrast medium in the retroperitoneum outlining the right kidney; a mottled air and soft tissue density in the right anterior pararenal space due to leak of duodenal contents, bile and air; or both of these findings together. With ultrasonography, a right paranephric fluid collection or an inflammatory mass adjacent to the right kidney or both may be better visualized. Computed tomography, the definitive study, delineates the true extent of the fluid or mass in the anterior pararenal space. Early detection by the radiologist is mandatory to minimize morbidity. To make this diagnosis early requires a high index of suspicion when reviewing the abdominal radiographs of those patients with pain during, or a few hours after, sphincterotomy.

Aged↗
PubMed1986

Radiologic assessment of bile duct complications in liver transplantation: initial experience.

Four of the first 24 patients who received 31 orthotopic liver transplantations in our institution developed complications at the site of the surgical anastomosis in the common bile duct. There was one anastomotic stricture, one anastomotic stricture with "bile sludge" deposition, and two cases of bile sludge formation without stricturing at the anastomotic site. The two cases of stricturing required surgical revision while both those of bile sludge accumulation without stricture were successfully managed conservatively. Familiarity with the postoperative appearance of the common bile duct anastomosis and its complications is necessary for the proper assessment of these patients.

Adolescent↗
PubMed1985

Reconstitution of RecBC DNase activity from purified Escherichia coli RecB and RecC proteins.

The Escherichia coli RecB protein, normally synthesized in low amounts, has been amplified by linkage of the recB gene to the phage lambda leftward promoter in an expression plasmid. From strains harboring this plasmid, RecB protein has been purified to homogeneity by a simple procedure which includes affinity chromatography on a column of RecC protein bound to agarose. The purified RecB protein has DNA-dependent ATPase activity but no exonuclease activity. RecC protein alone has neither ATPase nor exonuclease activity. However, when combined together, the RecB and RecC proteins show the ATP-dependent double-stranded exonuclease properties characteristic of the RecBC DNase.

Adenosine Triphosphatases↗
PubMed1985

High resolution ultrasound of macro-orchidism in mental retardation.

Asymptomatic testicular enlargement is common in mentally retarded males. This enlargement includes males with non-specific forms of mental retardation as well as a subgroup of males who have a chromosomally recognizable feature involving the X-chromosome ("fragile X" chromosome). We examined the testes of a group of 25 mentally retarded males with macro-orchidism including both fragile X and non-fragile X patients. Apart from large size, the testes scanned were all normal in appearance. In particular, there was no evidence for neoplasia. There were no distinguishing features between the fragile X and the non-fragile X groups.

Evaluation Studies as Topic↗
PubMed1985

Pleural effusion following liver transplantation.

The postoperative chest radiographs of 11 patients who underwent 14 orthotopic liver transplantations were reviewed. Pleural effusions occurred on the right side in all patients and on the left side in four. The effusions were unrelated to primary cardiovascular disease and usually asymptomatic. They were transudates and resolved spontaneously.

Adolescent↗
PubMed1985

Postoperative abdominal wall hematomas have a distinctive appearance on ultrasonography.

In six patients a large abdominal wall hematoma developed 1 1/2 to 6 months after operation. In each case differentiation of incisional hernia from hematoma was difficult, but the distinctive appearance on ultrasonography of an ovoid mass with thick straight internal septae, characteristic of hematoma, was noted in all. Ultrasonography also showed the multilocular nature of the lesion which made percutaneous aspiration difficult.

Abdominal Muscles↗
PubMed1984

Molecular amplification and purification of the E. coli recC gene product.

The level of recC gene expression has been analysed using Mud(bla lac) fusions to the recC promoter. The constitutive level of expression is very low and remains so even under SOS inducing conditions. The recC gene product has been amplified by harnessing the gene to the phage lambda leftward promoter in a plasmid. From cells harbouring this plasmid, RecC protein, which represented approximately 6% of the total cellular protein, was purified to electrophoretic homogeneity.

Bacteriophage lambda↗
PubMed1984

Computed tomography in pelvic liposarcoma: a case report.

A case of well-differentiated pelvic liposarcoma with unusual presentation of low-back pain and sciatica, due to the herniation through the greater sciatic notch, is described. Computed tomography is invaluable, not only in predicting the histologic diagnosis, but also in outlining the location, extent, and size of the tumor. This allows accurate planning of the surgical approach.

Back Pain↗