Biomedical subjects
Publications and source records attributed to L I Globa.
The peculiarities of the absorption of phages T2 or MS2 on the Na-, Ca- or Al- forms of cherkassky montmorillonite have been studied. This depended on the size of the virus and the type of the exchanged cation of the mineral. The affinity of the phages to various forms of the montmorillonite has been defined and their maximum phage-sorptive activities determined.
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The index of hydrophobicity and the hydrophilic-hydrophobic properties of seven microbial cultures were determined by studying their adhesion to n-hexadecane. The index of hydrophobicity was shown to be a stable characteristic of a culture grown in media with different carbon sources. As was found for Escherichia coli K-12 and Acinetobacter calcoaceticus K-9 whose hydrophobicity indicates were quite different, the character of cell hydration was virtually independent of the growth phase and did not change upon either submerged or superficial cultivation.
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Conditions were studied for the coagulation of aggregation-stable Bacillus subtilis, Flavobacterium rigens and Escherichia coli cell dispersions. Their aggregative stability decreased when montmorillonite, a clay mineral, was added to a weakly acid medium or in the presence of Al3+ ions. Bacterial heterocoagulation under the action of montmorillonite can be used as a universal strategy for biomass isolation from aqueous dispersion media.
Candida guilliermondii and Escherichia coli cells were adsorbed on glass and basalt fibres with a similar specific surface, but with a different charge. The quantity of adsorbed microorganisms did not depend on the type and charge of a fibre surface. However, cells were adsorbed faster and more firmly on positively charged and uncharged fibres than on negatively charged fibres.
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Adsorption of E. coli B phage T2 by natural Cherkassy montmorrillonite from water under isothermal conditions was studied. This natural Cherkassy montmorrillonite was found to be superior by its specific adsorption capacity to known materials for removal of viruses from water and therefore to be a prospective sorbent for treatment of water aimed at removal of viruses from it.
The described installation for microelectrophoresis is distinguished by the open-type electrode cameras. Platinum electrodes now employed considerably simplify its construction and operation. The design of this installation allows easy replacement of damaged blocks. It is recommended for studying the electrokinetic properties of microorganisms, blood cells and other dispersed particles.