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Biomedical subjects

L I Vaneeva

Publications and source records attributed to L I Vaneeva.

At least 19 recordsLinked to original sources
PubMed1991

[Optimization of the parameters that permit improvement in plate sorption activity for solid-phase immunoenzyme analysis].

The possibility of increasing the sorption activity of polystyrene plates with an initially low capacity for sorption has been shown. The qualitative and quantitative parameters of the process of physical modification have been ascertained. The empirical formula for achieving the highest degree of the sorption activity of plates by their definite exposure to ultraviolet radiation has been obtained.

Adsorption↗
PubMed1991

[The use of the method of the complete advisability function for evaluating the quality and standardization of the plates in solid-phase EIA].

An attempt to use the method of the complete advisability function for the evaluation of the quality of plates and their standardization in the enzyme-linked immunosorbent assay (ELISA) has been made. Correlations have been empirically established, thus making it possible to calculate the optimum proportions of different ELISA factors: the dose of the antigen (antibody) and the time of sorption, which ensures the best ELISA results with a given type of plates.

Enzyme-Linked Immunosorbent Assay↗
PubMed1990

[The common antigenic determinants of the H antigens of salmonellae].

Salmonella H-antigens have common and specific antigenic determinants. Studies on the determination of common antigenic determinants of H-antigens (a; b; d; i; 1,2 and nt) has been carried out. The order of the distribution of H-antigens according to the decrease of these common antigenic determinants in size is presented: d greater than a greater than 1,2 greater than b greater than nt. These data broaden our knowledge of the structure of H-antigens; they are also necessary for obtaining specific antibodies and conjugate preparations used in the enzyme immunoassay.

Antigens, Bacterial↗
PubMed1989

[The development of methods for obtaining monospecific ingredients for immunoenzyme analysis].

The methods of the modification of Salmonella O- and H-antigens and the preparation of biologically active sorbents on their basis have been developed. The use of these sorbents has permitted the isolation of affinity antibodies with strictly defined specific activity. The work shows the possibility of the successful use of carriers obtained on the basis of porous glass, chemically modified by acrylic copolymers containing activated carboxylic groups, and intended for the immobilization of antigens of both protein and carbohydrate nature.

Animals↗
PubMed1989

[Antigen-antibody complex dissociation in affinity chromatography].

Different methods of the elution of antibodies from an immunosorbent column have been studied, the serological activity and affinity of eluted antibodies have been evaluated in the enzyme immunoassay. The activity of conjugates (peroxidase-labeled antibodies) has been shown to depend not only on the serological activity and affinity of antibodies, but also on the method of binding antibodies with the enzyme.

Adsorption↗
PubMed1987

[Specificity of peroxidase-labeled antibodies to rabbit, mouse and human immunoglobulins].

The comparative study of different types of conjugates (antirabbit, antimouse and antihuman) has shown that gamma globulin fractions actively interact not only with homologous peroxidase-labelled antibodies, but also with heterologous ones. Cross reactions were most pronounced between antimouse conjugates and the preparations of human gamma globulin and, vice versa, between antihuman conjugate and mouse gamma globulin. The study has shown that the main cause of cross reactions is the presence of common antigenic determinants in the preparations of mouse and human gamma globulin.

Animals↗
PubMed1987

[Isolation and study of active peptides in Salmonella H antigens].

The monomer forms of Salmonella H-antigens a, b, d, i, 1, 2 have both specific antigenic determinants, characteristic of each H-antigen, and common determinants. The presence of two types of determinant groups leads to the appearance of cross reactions in the enzyme immunoassay. In this work the method for the isolation of peptides carrying only specific antigenic determinants is proposed.

Antigens, Bacterial↗
PubMed1987

[Problems of selecting the reagent concentration in the solid-phase immunoenzyme method for determining antibody concentrations].

The problems of selecting the concentration of reagents with the aim of increasing the accuracy and sensitivity of reactions, as well as decreasing the consumption of reagents, are shown as exemplified by the antigen-antibody reaction of the first order. A new approach to the ELISA reaction is proposed, which makes it possible to present the totality of consecutive interactions of the reagents as block diagrams described by known mathematical expressions. The limitations of the linear dependence of the results of the reaction are shown, and the methodological recommendations for overcoming these limitations are given.

Antibodies, Bacterial↗
PubMed1986

[The indirect solid-phase method of immunoenzyme analysis, its accuracy and sources of error].

The study of the accuracy of the enzyme-linked immunosorbent assay has revealed that the accuracy of this assay is low. The influence of instruments (dispensers, photometers, plates) on the accuracy of the assay has been studied. As shown in this study, the main sources are titration procedures and differences in the adsorption and optical properties of available plates, those manufactured in the USSR giving greater errors.

Adsorption↗
PubMed1986

[Sorption capacity of polystyrene plates used in immunoenzyme analysis].

The data indicating that the degree of sorption of the antigen onto the plate depends on the duration of this process are presented. As shown in this investigation, the lower the adsorption activity of the plates, the longer the process of the sensitization of plates with the antigen. The process of the sorption of the antigen onto the plates with low adsorption capacity may be accelerated by sensitizing the plates at different temperatures. Besides, the adsorption capacity of the plates was increased by ultraviolet irradiation.

Adsorption↗
PubMed1985

[Rapid immunoenzyme analysis].

A variant of the immunoenzyme assay, reducing the duration of the assay procedure by 3 hours in comparison with the standard technique, is presented. This rapid variant is sufficiently sensitive and reproducible.

Antigens, Bacterial↗
PubMed1985

[Development of a method of obtaining peroxidase conjugates with anti-immunoglobulin for immunoenzyme analysis].

The results of the successive stages of preparing peroxidase-labeled anti-immunoglobulins are presented. The schedules for the immunization of animals have been worked out with a view to prepare antisera to human gamma globulin and, subsequently, to isolate antibodies from them by different methods. The preparations obtained with the use of immunosorbent have proved to possess the highest serological activity. As the result of these investigations, a new method of binding, a modification of the glutaraldehyde method, has been proposed. The conjugates obtained by the proposed method are characterized by high serological activity, stability and good reproducibility.

Animals↗
PubMed1978

[Immunochemical study of subtilisins obtained from Bacillus subtilis A-50 and some of its mutants].

Physico-chemical parameters of subtilisins from the original Bacillus subtilis A-50 strain (proteolytic activity, electrophoretic mobility, molecular weight, reactions with specific inhibitors) were similar to those mentioned in the literature for the enzymes of other strains. Immunological experiment has shown, that Bacillus subtilis A-50 subtilisins with various electrophoretic mobility do not differ in their antigenic properties. Enzymes with high electrophoretic mobility from mutant strains were similar to I--III subtilisin fractions from Bac. subtilis A-50 in the antigenic characteristics. However, the antigenic heterogeneity was observed in I, II and III enzyme fractions of some mutant strains. Subtilisins studied appear to form the isoenzyme system.

Antigen-Antibody Reactions↗
PubMed1978

[Study of hemosensitization characteristics for the purpose of standardizing erythrocyte flagellin diagnostica].

The work is devoted to the study of conditions required for the purpose of creation of standard and the most active preparations of erythrocytic H-diagnostic agents. The dependence of binding of the H-antigen by erythrocytes on the pH and the ionic power of the medium was investigated. It was demonstrated that in the process of erythrocyte sensitization with flagellin of great importance were electrostatic, hydrogen, and hydrophobic powers.

Adsorption↗
PubMed1978

[Immunogenic properties of various forms of flagellin].

The authors obtained and studied immunologically flagellae of S. typhi and flagellins (monomeric and polymeric) obtained from them. Immunological activity of flagellin was the greater the higher the molecular weight of the preparation (the polymer molecules were the largest and the most immunogenic). There were revealed differences between the preparations according to the dependence of the immunizing effect on the dose and the capacity to induce synthesis of 19S- and 7S-antibodies. A sharp change of the immunogenicity of flagellin preparation following gel-filtration through sepharose 2B was demonstrated.

Animals↗
PubMed1978

[Immunogenicity of H-antigen polymers obtained by different methods].

The authors studied the properties of two polymeres prepared from flagellin by various methods: precipitation with ammonium sulfate with the subsequent centrifugation or treatment with glutaric aldehyde in solution and subsequent gel-chromatography. Molecules of the former (P-I) and of the latter (P-II) were similar by shape, but the mol wt of P-I was greater. The preparations contained common H-antigen and were similar by serological activity. P-I had a very high "priming" activity for mice. P-II was highly immunogenic only within the narrow range of doses, was less immunogenic in comparison with P-II, and, in difference from P-I, induced formation of some amount of 7S-antibodies.

Animals↗