Biomedical subjects

L J McCarty

Publications and source records attributed to L J McCarty.

7 recordsLinked to original sources

PubMed1977

Heat-ethanol isolated serum albumin: discussion.

Albumin isolated by the Hagen/Münster heat-ethanol method is a remarkably stable solution of high purity and clinical compatibility. The simplicity of this method makes it a model fractionation procedure for the small transfusion service. Immunization studies carried out by us are compared with previous accounts of a "new" antigen occuring in plasma protein solution produced by partial heat denaturation. Clinical compatibility of cold-ethanol and heat-ethanol fractionated albumin solutions are also shortly discussed.

Antibody Formation↗

PubMed1976

Technical improvements in heat-ethanol isolation of serum albumin.

Although the heat-ethanol method of fractionation for the isolation of normal serum albumin (NSA) is technically easier and more economic than cold-ethanol methods, globulin removal and albumin concentration remain basic hinderances to a truly simplified procedure. Continuous flow centrifugation for the separation of precipitated proteins has been effectively replaced on the one hand by alluvial filtration (globulins), and onthe other hand by diafiltration (albumin). Through these changes, investive and running costs are further reduced, noise is all but eliminated, albumin yield is somewhat increased, and personnel required for a 600 1 plasma batch is reduced from four workers to one.

Ethanol↗

PubMed1976

Alternatives for plasma fractionation.

At the present time there is an enormously increasing demand for albumin. The most common procedure for the isolation of this plasma component is the cold ethanol technique developed by Cohn. Because this process necessarily isolates other blood components for which there is less demand in relation to albumin, albumin production is expensive. Therefore, we have developed a two-step fractionation for the isolation of albumin. It is basically a heat precipitation method with the albumin yield being about 90% of the original plasma albumin. In comparison to cold ethanol methods, it is considerably less expensive. Other blood components, e.g., clotting factors, immunoglobulins, may also be isolated. A nonmodified gamma-globulin for intravenous use is obtained by removing anti-complementary activity with hydroxyethyl starch. Additional fractionation steps are required to isolate these other components, but unlike in established methods, these are not necessary for the isolation of solely albumin.

Chemical Fractionation↗

PubMed1976

Isolation of Non-modified gamma globulin for intravenous use.

The availibility of a non-modified gamma globulin for intravenous use should abolish the intramuscular administration of Cohn standard gamma globulin and replace modified products to the greatest possible extent. The product developed in our institute is purified from Cohn fraction II using HES and aluminum silicate for the elimination of aggregates. The product shows excellent purity, stability, and clinical compatibility.

Aluminum Silicates↗

PubMed1975

An alternative method of large scale plasma fractionation for the isolation of serum albumin.

Human plasma may be separated into five fractions using the method described by Cohn in 1946. Although there are several drawbacks to alcohol precipitation, especially in albumin isolation, it is still used throughout the world. This paper describes an alternative procedure for albumin isolation from plasma or albumin-containing plasma fractions using a combined heat fraction/polyethylene glycol precipitation method. No polyethylene glycol is detected in the final product which is immunoelectrophoretically 100% pure, salt poor, heat resistant during pasteurization, and stable during long-term room temperature storage. The yield is at least 90% of the original plasma albumin. In comparison with the Cohn method, fractionation time and expense are significantly reduced.

Chemical Precipitation↗

PubMed1975

Gamma-D paraproteinemia - a report of 16 cases.

Paraproteins of the gammaD class are very rare, being found in only 0.3% to 3.0% of the cases of all paraproteinemias. They are difficult to detect and therefore sometimes not found or mis-diagnosed. In the 6500 cases of paraproteinemia we have diagnosed over the last eight years, 16 of them have been gammaD. Consistent with the reports of other gammaD paraproteinemias most of these 16 cases were of type L (13), most patients were male (11), the average age (55), was younger than that of patients suffering from other paraproteinemias, and in all cases the disease followed a rapidly malignant course.

Adult↗

PubMed1975

Frozen-thawed red cells--standard article for hospital use?

The freezing and storing of glycerol protected red cells allows the present 20---40 day limitation of red cell storage to be indefinitely lengthened. Although frozen thawed cells are more expensive to prepare than ordinary packed cells, the longer shelf-life of the red cells, the reduced risk of hepatitis transmission, and the reduction of immunization against leuko- and thrombocytes, are all advantages which out-weighed this additional cost. To effect an increase in their use, frozen thawed cells could be distributed from central blood banks to hospitals were they would be stored until needed for transfusion. Thawing and washing would necessarily be done by the hospital blood bank personnel.

Blood Banks↗