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Biomedical subjects

L J Turton

Publications and source records attributed to L J Turton.

5 recordsLinked to original sources
PubMed1983

Effect of glucose concentration in the growth medium upon neutral and acidic fermentation end-products of Clostridium bifermentans, Clostridium sporogenes and peptostreptococcus anaerobius.

Clostridium bifermentans, C. sporogenes and Peptostreptococcus anaerobius were grown in Fastidious Anaerobe Broth with glucose concentrations from 0-1.5% (w/v). Gas chromatographic (GLC) analysis of fermentation end-products revealed that a change in glucose concentration markedly affected fermentation patterns of each microorganism. Increased glucose concentration resulted in stimulation of ethanol production by all strains, and of butanol and butyric acid by C. sporogenes. With C. bifermentans, there was a parallel increase in ethanol and acetic acid production and decreased formation of propanol and propionic, iso-butyric and iso-valeric acids. Increased ethanol formation by P. anaerobius was accompanied by an increase in acetic acid and a decrease in butan-1-ol production. The greatest incremental change in GLC profiles occurred when glucose concentration was raised from zero to 0.1% (w/v). These data suggest that glucose concentration in a medium for GLC analysis must be rigorously controlled if reproducible results are to be obtained.

Chromatography, Gas↗
PubMed1983

Effect of eight growth media upon fermentation profiles of ten anaerobic bacteria.

A study was made of the influence of eight growth media upon the fermentation end-product patterns obtained with ten species of anaerobes. Acidic and neutral end-products of fermentation were analysed by gas chromatography and the results were examined statistically by computer. Differences in end-product profile were at least as great between different media used to grow a single anaerobic species, as between different anaerobes grown in a single type of medium. When individual fermentation end-products produced by a single anaerobe species were examined statistically significant differences were found between different media for individual end-products. It is concluded that standardisation of growth medium is essential in diagnostic laboratories concerned with identification of anaerobes with the aid of gas chromatography.

Anaerobiosis↗
PubMed1982

Evaluation of Fastidious Anaerobe Broth as a blood culture medium.

Three commercial blood culture media were compared with a freshly prepared cooked meat medium in tests to stimulate the recovery of small inocula of anaerobic and aerobic bacteria in routine blood cultures. The cooked meat medium gave the most reliable recovery and supported continued viability, whilst Fastidious Anaerobe Broth (LAB M) was a good alternative. Results with Southern Group thioglycollate and Difco Thiol were less satisfactory as delays in recovery and loss of viability occurred on continued incubation with some of the test strains.

Bacteriological Techniques↗
PubMed1982

Effect of incubation time, and calcium carbonate and glucose in the growth medium, upon the fermentation end-product profile of Clostridium difficile.

The effects of time, glucose and calcium carbonate upon fermentation profiles of Clostridium difficile were determined. The test organism was grown in three media, viz. fastidious anaerobe broth (FAB), FAB with 1% w/v glucose, and FAB with 1% glucose plus calcium carbonate. Fermentation products were analysed after 24, 48 and 72 h incubation. By 48 h, qualitatively 'typical' profiles could be produced. Glucose supplementation of FAB suppressed formation of certain branched-chain carboxylic acids. However, the presence of calcium carbonate, in addition to glucose, resulted in a quantitative increase in products after 48 h and 72 h. This effect was particularly marked with respect to alcohol production by C. difficile.

Calcium Carbonate↗