PubMed Health⌕ Search

Biomedical subjects

L J Wilson

Publications and source records attributed to L J Wilson.

45 records · Page 3Linked to original sources
PubMed1983

Localization of messenger RNA in the cortex of Chaetopterus eggs and early embryos.

The distribution of mRNA in Chaetopterus pergamentaceus eggs was examined by in situ hybridization with poly(U) and specific cloned DNA probes. Eggs contain three distinct regions; the cortical ectoplasm, endoplasm, and a plasm released from the germinal vesicle (GV) during maturation. The ectoplasm of the mature egg showed a 15-fold enrichment in poly(A) and in histone and actin mRNAs relative to the endoplasm and the GV plasm after in situ hybridization. More than 90% of the total mass of egg poly(A) + RNA and histone and actin messages was estimated to be present in the ectoplasm. The mRNA molecules co-distributed with ectoplasmic inclusion granules during ooplasmic segregation. During the extensive cytoplasmic rearrangements which occur at the time of the first cleavage the ectoplasm was divided into animal and vegetal portions. The animal portion was segregated evenly between the AB and CD blastomeres, whereas the vegetal portion entered the polar lobe and was preferentially segregated to the CD blastomere. Histone and actin mRNA entered both the AB and CD blastomeres of the 2-cell embryo. The results demonstrate that mRNA is quantitatively localized in the cortex of the Chaetopterus egg and early embryo.

Animals↗
PubMed1983

Ten central elements of family practice.

Previous studies of the content of family practice have analyzed the discipline in terms of the clinical problem content. Taking a different approach, a study group analyzed the care given to patients by family physicians irrespective of the specific clinical problems. Working with a reference group of family physicians in private practice, ten central elements were identified: (1) comprehensiveness of care, (2) anticipation of problems and continuity of care, (3) personal relationships with a patient, (4) medical knowledge and skills characteristic of family medicine, (5) values and attitudes that enhance family medicine, (6) problem definition and medical decision making, (7) problem management and resource coordination, (8) care of the individual within the family context, (9) involvement with the community, and (10) attentiveness to practice organization. This study provides a different point of departure for the design and evaluation of educational programs in family practice.

Adult↗
PubMed1979

Sexual and marital problems in a psychiatric out-patient population.

The study aimed to identify those patients referred to a psychiatric out-patient clinic who had sexual or marital difficulties and who could be offered help with these problems, although none of the patients had been referred primarily for sexual or marital therapy. The methods of assessment formed a series of filtering devices for screening the sample, and possible reasons for discrepancies in the results are discussed. Twelve per cent of the sample had sexual or marital problems and were offered treatment, and it is suggested that this proportion may be an underestimate of the 'true' frequency of such problems in the sample. Implications of this finding for the provision of treatment resources in the psychiatric out-patient setting are considered.

Adult↗
PubMed1978

Electronic state of heme in cytochrome oxidase III. The magnetic susceptibility of beef heart cytochrome oxidase and some of its derivatives from 7-200 K. Direct evidence for an antiferromagnetically coupled Fe (III)/Cu (II) pair.

The temperature dependence of the paramagnetic susceptibility of cytochrome oxidase and some of its derivatives has been measured from 7 to 200 K. The results obtained for the fully oxidized (resting) enzyme correspond exactly to the requirements of the model recently proposed by Palmer et al. (Palmer, G., Babcock, G. T., and Vickery, L. E. (1976) Proc. Natl. Acad. Sci. U. S. A. 73, 2206-2210) in which the enzyme possesses two magnetically isolated spin S = 1/2 centers and a spin-coupled S = 2 center. The S = 2 center paramagnetism has been interpreted as arising from a [cytochrome a33+(S = 5/2)--Cuu2+(S = 1/2)] antiferromagnetically coupled iron.copper binuclear complex of total spin S = 2 with -J greater than or equal to 200 cm-1. In addition, the wide temperature range used in the present studies has permitted an analysis of present and other available data (T less than 4K measurements) which readily accommodates results from this and other laboratories (Moss, T.H., Shapiro, E., King, T.E., Beinert, H., and Hartzell, C. R. (1978) J. Biol. Chem 253, 8072-8073) so that a fully consistent picture of the magnetic centers in cytochrome oxidase now appears to be available. Furthermore, anomalous magnetic behavior for the oxidized enzyme.cyanide complex has been interpreted in terms of an antiferromagnetic exchange interaction operating in the binuclear complex [cytochrome a33+.CN-(S = 1/2)--Cuu2+(S = 1/2)] with -J congruent to 40 cm-1. A structural model for the [cytochrome a3(3+)-bridge-CUu2+] center is advanced in which an imidazolate ion serves as the bridging ligand in a manner similar to that found in superoxide dismutase.

Animals↗
PubMedDate not supplied

An epidemiologic study of fatigue with relevance for the chronic fatigue syndrome.

We surveyed households in four rural Michigan communities to confirm a reported cluster of cases resembling chronic fatigue syndrome (CFS) and to study the epidemiology of fatigue in a rural area. Data were collected from 1698 households. We did not confirm the reported cluster. The prevalence of households containing at least one fatigued person was similar between communities thought to harbor the cluster and communities selected for comparison. Symptoms and features of generic forms of fatigue were very similar to those often attributed to CFS.

Adolescent↗