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Biomedical subjects

L Jäger

Publications and source records attributed to L Jäger.

At least 109 records · Page 6Linked to original sources
PubMed1985

[The quantitative determination of the IgM rheumatoid factor using a solid-phase radioimmunoassay. Comparison with the agglutination test and the radioimmuno-polyethyleneglycol precipitation test].

A solid-phase radioimmunoassay capable of detecting nanogram quantities of human IgM rheumatoid factor using a monoclonal anti-mu-chain antibody is described. Human IgG did not interfere with the detection of IgM RF by this method. The small nonspecific binding of nonRF IgM to the human IgG coated tubes utilized in the assay must be corrected for by assaying samples in parallel bovine serum albumin coated control tubes only in cases of deviation of IgM from normal range. 69 coded and randomly arranged sera from patients with rheumatoid arthritis (RA), nonrheumatic joint diseases and healthy adult control subjects were investigated by this method, agglutination techniques as well as RIPEGA. A good correlation between solid-phase radioimmunoassay and agglutination techniques was found. Patients with seropositive RA had significantly higher concentrations of IgM RF than seronegative RA patients or control subjects (mean +/- 1 SD = 133,3 +/- 187,2 micrograms/ml versus 4,7 +/- 6,5 micrograms/ml and 2,2 +/- 4,0 micrograms/ml; resp.).

Agglutination Tests↗
PubMed1984

[Diagnostic and therapeutic aspects in the treatment of bee venom allergy].

In 50 patients who had severe allergic general reactions after a bee sting clinical and immunological parameters were examined under the desensitisation with apitoxin. Under the introduction of the hospital treatment 25 patients showed side effects. 14 of these patients had milder general reactions also during the further continuation of the ambulatory treatment. In no case the treatment had to be stopped. The behaviour of the specific IgE and IgG antibodies does not allow a prognostic evidence concerning possible side effects and is little suited for the individual case also as objective parameter of success. 17 patients who during the first weeks after the beginning of the therapy spontaneously had been stung by a bee did not show any reactions or only reduced reactions. This result shows that the desensitization of desensitized patients gives a secure protection from life-threatening incidents. Open remains among others the problem of the objective judgment of the therapy and thus the question of the duration of the treatment.

Adolescent↗
PubMed1984

[Identification of allergens in 5 grasses using crossed radioimmunoelectrophoresis (CRIE)].

Using crossed radioimmunoelectrophoresis ( CRIE ) aqueous extracts from pollen of Phleum pratense , Lolium perenne, Poa pratensis , Festuca pratensis and Alopecurus pratensis were investigated for allergen composition. We detected between 24 and 32 antigens. Employing sera from 11 patients with well established hay fever, IgE binding could be demonstrated in 15 out of 28 antigens in Phleum pratense , 13 out of 32 in Lolium perenne, 14 out of 26 in Poa pratensis , 12 out of 24 Festuca pratensis and 12 out of 24 antigens in Alopecurus pratensis . The 11 patients showed an individual pattern of sensitization against the various pollen allergens.

Allergens↗
PubMed1983

Marker and functional characterization of immunoregulatory cells in rheumatoid arthritis.

In 34 patients with rheumatoid arthritis we determined the regulatory index OKT 4: OKT 8 and the suppressor index delta 24: delta 0. There is no correlation between the results of the detection of subpopulations with monoclonal antibodies and of the functional test to determine the suppressor activity. The enhanced mitogen-induced proliferation after 24-h preincubation is regularly associated with a rise in the regulatory index; however, without convincing quantitative correlation. It can be concluded, that only a small portion of the cell population identified by monoclonal antibodies OKT 4 and OKT 8 exerts helper or suppressor activities. At present, identification of regulatory T-cell subpopulation should be complemented by functional tests.

Antibodies, Monoclonal↗
PubMed1982

Lymphocyte activation by low doses of cyclosporin A.

Supernatants of human lymphocyte cultures incubated with cyclosporin A (CS-A) for 90 min reveal an inhibitory effect on the electrophoretic mobility of guinea-pig macrophages. This effect is distinctly demonstrable already at CS-A concentrations from 10-7 micrograms/ml on with a maximum at 10-3 micrograms/ml. An inhibitory action of CS-A on PHA-induced lymphocyte proliferation is detectable only beyond 10-2 micrograms/ml CS-A. The CS-A induced lymphokine is comparable to that stimulated by other ways (PHA, ConA).

Animals↗
PubMed1980

[Significance of the immune complex reaction in internal medicine].

A review is given concerning occurrence, identification, and pathogenetic effects of immune complexes. The formation of immune complexes by interaction of antigen with antibody, is a component of the normal immune response. In cases with inefficient clearance by the mononuclear phagocyte system only, pathological consequences will be expected, in particular by immune complexes formed with moderate excess of antigen. For the identification of immune complexes in biological fluids many methods have been developed. The methods are based mostly on biological activities of immune complexes, e.g. interaction with complement or rheumatoid factor and reactivity with cellular receptors. These antigen-nonspecific methods do not allow to discriminate between true immune complexes and nonspecifically aggregated immunoglobulins. Circulating immune complexes become fixed to basement membranes of the body. They can produce an acute inflammatory reaction by activation of complement and inflammatory cells and also interfere with the immune response. Circulating immune complexes have been detected in some human diseases, e.g. autoimmune diseases (LEV, RA), infectious diseases, malignancies, serum sickness syndrome, immune-complex glomerulonephritis, and after transplantation. The possibilities for management of immune complex diseases are discussed.

Antigen-Antibody Complex↗
PubMed1980

[Clinical indications for monitoring immunologic reactivity (immune status)].

The tests and the clinical indications for monitoring immunologic reactivity are discussed. From the viewpoint of reliability in relation to diagnosis and therapy for the single patient there are some basic indications: the primary and secondary immunodeficienceis, the immunoproliferative disorders and the monitoring during immunotherapeutic procedures. In autoimmune diseases and in cancer these investigations do not give sufficient information concerning immunoreactivity of the single patient. The differences are statistical significant only for groups. In the future antigenic specific tests seem to be more promising.

Autoimmune Diseases↗
PubMed1980

[Determination of the MSF (macrophage slowing factor) release by phytohemagglutinin using the macrophage electrophoretic mobility test (MEM test). I. Dependency of release on incubation time, phytohemagglutinin and cell concentration].

Under the influence of phytohaemagglutinin (PHA) lymphocytes of the peripheral blood from healthy persons and patients without immunodeficiencies produce a lymphokine called macrophage slowing factor (MSF). Kinetic studies of MSF production according to time-dependence, dose-response-effect and lymphocyte concentration revealed the optimal test conditions: 90 min resp. 4 h incubation time, 1 microliter and 5 microliter PHA per test and 10(6) lymphocytes per test. These conditions provided a decrease of 11.0 +/- 4.1% of macrophage mobility in the electric field. The PHA-model can be used as a positive control for the MEM-test. Moreover the PHA-model serves as a test for immunological reactivity.

Cells, Cultured↗
PubMed1979

[Salicylamide-- a potential pain-remedy alternative in the analgesic asthma syndrome].

Central pathogenetic mechanism of the analgesics-asthma-syndrome is very probably an analgesics-conditioned inhibition of the prostaglandin-biosynthesis (cyclooxygenase). Salicylamide, an analgesic without effect on the prostaglandin synthesis was in the oral exposition test of 18 patients with proved analgesics-asthma-syndrome in the dosage of 500 mg subjectively and objectively tolerated without reaction. Body-plethysmographically in consistent thoracic gas volume a slight decrease of the airways resistance could be registered. At the model of the isolated trachea of guinea-pigs salicylamide, in contrast to indomethacin and phenazone (prostaglandin-synthesis inhibitory substances) did not lead to an increase of the acetylcholine-induced spasm. Salcylamide can, after a tolerability test, be recommended as alternative analgesic in the analgesics-asthma-syndrome in a dosage of 500 mg.

Analgesics↗
PubMed1979

[House-dust allergy in bronchial asthma].

1. House dust allergy and house dust mite allergy are with 18.4 and 14.1% the most frequent causes of the atopic asthma also under our conditions. 2. In about 60% the house dust allergy is conditioned by a sensitization by constituents of mites (excretions). 3. The responsible antibodies may be established by means of the radio-allergo-sorbent-test. In about 50% results were found corresponding with the intracutaneous test. 4. In the restricted possibilities of the allergen carency the hyposensitization plays an important role. In critical choice of the patients in 50-60% a clinical improvement is to be achieved.

Animals↗
PubMed1979

[Asthma caused by analgesics].

Asthma, caused by analgesics, a relatively frequent phenomenon in bronchial asthma conditioned by infection, often remains unrecognized and is then sometimes a danger of high degree for these patients. Pathogenetically, the asthma caused by analgesics perhaps does not underlie an immunological mechanism, but an induced by analgesics prostaglandin-E-synthesis inhibition. For this speak the results of skin tests, of the LTT with analgesics and of IgE concentration measurements. Concomitant phenomena of the asthma caused by analgesics are: initial rhinirrhoe, alcohol intolerance, polyposis nasi and NNH affections. Among the evoking noxae the pyrazolones (regard: pyrazolone containing asthma mixed preparations!) are of greatest importance. Since already the smallest doses of analgesics may evoke considerable asthmatic reactions the indication to the exposition test should be made cautiously. In an anamnestic suspicion on asthma caused by analgesics a strict avoidance of analgesics, antirheumatic drugs and antipyretic drugs is necessary. Salicyl amide does not cause in vitro a prostaglandin synthesis inhibition. Still further clinical examinations are necessary, whether it amy generally be recommended as a possible alternative of analgesics in asthma caused by analgesics.

Analgesics↗
PubMed1979

[New viewpoints in the pathogenesis and therapy of bronchial asthma].

The bronchial asthma is a polyetiologic syndrome with increased bronchomotoric excitability. The most important forms are the allergic asthma, the infection-conditioned one, the asthma conditioned by analgetics, the exertion and the irritation asthma. Apart from the direct affections on the smooth musculature and other target cells reflectorical mechanisms play an essential part. The first above all operate in the smaller respiratory tracts and stand in the foreground in the allergic bronchial asthma. The latter have above all an influence on the larger bronchi and are to be found in a typical way in irritation asthma. However, the transitions are fluent. The differentiation of the responsible mechanisms and the localisation of the bronchial obstruction give the possibility of a more aimed treatment. An essential improvement of the -- symptomatic -- treatment might be achieved, when the factors of the increasing bronchomotoric excitability are better known.

Acetylcholine↗