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Biomedical subjects

L Johannesen

Publications and source records attributed to L Johannesen.

6 recordsLinked to original sources
PubMed1980

Variable expression of delayed hypersensitivity in different mouse strains using dimethyl dioctadecyl ammonium bromide as an adjuvant.

Delayed-type hypersensitivity measured as footpad swelling was studied in large number of inbred mouse strains. A conjugate of bovine serum albumin (BSA) with the small 2,4-dinitrophenyl (DNP) hapten served to generate strong reactions, specific for the DNP group. Delayed hypersensitivity was produced with the DNP-BSA complex mixed with the cationic, surface active lipid, dimethyl dioctadecyl ammonium bromide (DDA). Great variation was observed in delayed hypersensitivity among different mouse strains. For convenience, the mice were classified into five groups, notably: non-, low, moderate, good and high responders. The highest responding animals were BALB/cJ mice, the lowest were P/JN and outbred nu/nu mice. No correlation was observed between H-2 type and the intensity of the elicited reactions.

Adjuvants, Immunologic↗
PubMed1978

Specificity of murine delayed-type hypersensitivity to conjugates of large or small haptens on protein carriers bearing lipid groups.

Delayed-type hypersensitivity (DH) in the mouse was provoked with different hapten-carrier complexes mixed with the cationic, surface-active lipid, dimethyl dioctadecyl ammonium bromide (DDA). DH was measured as footpad swelling. Conjugates of bovine serum albumin (BSA) with the small haptens dinitrophenyl (DNP), 'arsonate' (ARS) and 'sulphonate' (SULPH) served to generate strong DH reactions towards the homologous antigen. Insertion of a tripeptide spacer between the hapten and carrier resulted in lower DH reactivity. Optimal dosages and optimal time intervals between sensitization and DH elicitation were determined for the enlarged hapten-carrier complexes. Cyclophosphamide (CY) treatment, before priming with complexes mixed with DDA, caused a 5-6 day delay in the expression of DH but failed to evoke enhanced DH for any of the antigens tested. A broad array of cross reactions between small and enlarged hapten-carrier complexes showed a relative lack of specificity in these DH responses. The results are compared with others reported in the literature and are explained mainly by the effects of electrostatically bound lipid groups of DDA in the sensitizing conjugates.

Animals↗