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Biomedical subjects

L K Hansen

Publications and source records attributed to L K Hansen.

At least 73 records · Page 4Linked to original sources
PubMed1990

Crystallization and preliminary X-ray crystallographic studies of benzamidine-inhibited trypsin from the North Atlantic salmon (Salmo salar).

Crystals of benzamidine-inhibited trypsin from the North Atlantic salmon (Salmo salar) have been grown from ammonium sulphate solution at pH 5.0. Two crystal forms suitable for X-ray structure analysis, obtained from a hanging-drop experiment, have been characterized. Both belong to space-group P22(1)2(1) with cell dimensions a = 39.2 A, b = 62.4 A, c = 84.6 A and a = 31.4 A, b = 74.8 A, c = 83.5 A, for forms I and II, respectively. Intensity data to 1.82 A have been collected for crystal form I on a CAD4 diffractometer, and initial phases have been obtained by molecular replacement methods. The conventional R-factor after two rounds of model building and subsequent refinement is 0.25 for data between 6.0 and 2.0 A. So far no water molecules have been included in the model.

Amidines↗
PubMed1990

The relationship of white blood cell count to other cardiovascular risk factors.

Baseline data for the 12,866 men from the Multiple Risk Factor Intervention Trial was used to study factors related to white blood cell (WBC) count. White blood cell count was significantly higher in smokers (7853 cells/mm3) and ex-smokers (7091 cells/mm3) who stopped smoking less than one year before than in ex-smokers who stopped more than one year before (6255 cells/mm3) and those who never smoked (6094 cells/mm3). In current cigarette smokers, white blood cell count was significantly related to number of cigarettes smoked, degree of inhalation, and duration of smoking (p less than 0.001 for each). In addition, white blood cell count was higher in non-cigarette smokers who smoked pipes, cigars, or cigarillos than among men who did not smoke tobacco (p less than 0.001). White blood cell count was lower in blacks (by 877 cells/mm3) and Orientals (by 634 cells/mm3) than in whites. Leukocyte count also showed a strong inverse association with high density lipoprotein (HDL)-cholesterol, a positive association with triglycerides independent of cigarette use, and a positive association with low density lipoprotein (LDL)-cholesterol in smokers only. Leukocyte counts were inversely related to total family income and alcohol consumption. We conclude that elevated leukocyte count is independently associated with other risk factors for coronary heart disease (CHD) such as amount and duration of smoking as well as an atherogenic profile, and these relationships should be considered when using white blood cell count as a predictor of coronary heart disease.

Adult↗
PubMed1989

High-resolution (1.5 A) crystal structure of phospholipase C from Bacillus cereus.

Both the phosphatidylinositol-hydrolysing and the phosphatidylcholine-hydrolysing phospholipases C have been implicated in the generation of second messengers in mammalian cells. The phosphatidylcholine-hydrolysing phospholipase C (PLC) from Bacillus cereus, a monomeric protein containing 245 amino-acid residues, is similar to some of the corresponding mammalian proteins. This, together with the fact that the bacterial enzyme can mimic the action of mammalian PLC in causing, for example, enhanced prostaglandin biosynthesis, suggests that B. cereus PLC can be used as a model for the hitherto poorly characterized mammalian PLCs. We report here the three-dimensional structure of B. cereus PLC at 1.5 A resolution. The enzyme is an all-helix protein belonging to a novel structural class and contains, at least in the crystalline state, three Zn2+ in the active site. We also present preliminary results from a study at 1.9 A resolution of the complex between PLC and inorganic phosphate (Pi) which indicate that the substrate binds directly to the metal ions.

Bacillus cereus↗
PubMed1989

Bilateral female pelvic actinomycosis.

A case of bilateral actinomycotic salpingitis is described. The woman used an IUD and the course of the disease was protracted. Later, Actinomyces israelii was coincidentally identified from vaginal cultures. Attention is drawn to the need for distinction between the presence of Actinomyces israelii and actinomycosis.

Actinomycosis↗
PubMed1985

Low plasma fibronectin after drowning.

The plasma concentrations of fibronectin were zero for 8 days in a 53-year-old male who was submersed for 5 min, but conscious at the time of admission to hospital. The patient developed multiple organ failure, disseminated intravascular coagulation and finally died after 15 days. The low fibronectin values indicate prolonged severe reticulo-endothelial failure, and may be a prognostic sign in cases of drowning or asphyxia of other etiologies.

Combined Modality Therapy↗
PubMed1985

Buprenorphine-supplemented anaesthesia. Influence of dose on duration of analgesia after cholecystectomy.

Fifty-one patients undergoing cholecystectomy received a single dose of buprenorphine 4.5-12 micrograms kg-1 at induction. Median duration of analgesia after surgery was 6 h. Multiple regression analysis showed the duration of analgesia to be slightly dependent on the age of the patient. No relationship was found between the duration of analgesia and the weight-related dose of buprenorphine, the age, sex or body weight of the patient, the duration of anaesthesia, or the dose of droperidol or thiopentone administered.

Adjuvants, Anesthesia↗
PubMed1985

The Venturi anaesthesia circuit I. An all-purpose breathing system for anaesthesia.

The Venturi is a flow-accelerating injector, activated by fresh gas inflow from the anaesthetic machine. The Venturi entrains exhaled gas from the patient through a soda-lime canister, and carries it to the patient together with fresh gas. The Venturi circuit is a Mapleson D system and the fresh gas requirements are roughly 30 ml kg-1 min-1 for anaesthetized, relaxed, adult patients under controlled ventilation.

Adult↗
PubMed1985

Bile acid malabsorption in patients with an ileum reservoir with a long efferent leg to an anal anastomosis.

Biliary metabolism in 11 patients with ileum reservoirs with anal anastomosis and a long efferent leg was studied. Eleven healthy persons served as controls. A significantly higher excretion of bile acids was found in the patients, but they seemed to have a normal cholic acid pool size. The bile acids excreted were cholic acid and chenodeoxycholic acid, the so-called primary bile acids, for more than 90%, whereas the normal controls mainly excreted secondary bile acids (deoxycholic acid and lithocholic acid). Fat excretion was generally not increased in the group but was above normal in two patients. Vitamin B12 absorption was subnormal in two patients and was not correlated to bile acid excretion. Bile acid excretion was not correlated to the weight of feces. The bacterial flora was more feces-like than would have been expected from a normal terminal ileum but was correlated neither to the bile acid excretion nor to the quantity of feces. We conclude that the patients showed dysfunction of the terminal ileum with regard to biliary acid absorption comparable to that found in patients with partial ileal resections.

Adult↗
PubMed1985

The defecation pattern and diet in patients with an ileum reservoir and anal anastomosis with a long efferent leg.

The dietary habits and defecation patterns of 12 patients were examined 8-34 months after total colectomy with mucosal proctectomy and the construction of an ileal reservoir attached to an anal anastomosis. The reservoir was provided with a long efferent leg (12 cm) through the anal canal. The patients were divided into two groups in accordance with the frequency of defecation--one with fewer than six defecations per 24 h and another with more than six defecations per 24 h. The fecal output, however, was not significantly different in the two groups. The fecal content of sodium and potassium was the same as in feces from conventional ileostomies. The dietary intake was insufficient in energy content, vitamins, minerals, and trace elements. Dietary supplement was recommended.

Adult↗
PubMed1985

Total colectomy, mucosal proctectomy, and an ileal reservoir to an anal anastomosis. A comparison of short and long efferent legs.

In a consecutive series of 46 possible candidates for total colectomy, mucosal proctectomy, and ileal reservoir to an anal anastomosis, we have compared the clinical outcome of group I, with a long efferent leg (12 cm), and group II, with a short efferent leg (3-5 cm). The surgical procedure was done in three steps: first, a total colectomy; second, mucosal proctectomy and construction of an 'S-shaped' ileal reservoir with a temporary loop ileostomy; and, third, closing of the ileostomy. Nine patients with a long efferent leg and eight patients with a short leg were observed for 2-51 months with a functioning ileal reservoir. The overall mortality was zero. The results showed that the short efferent leg was important for low fecal urgency, spontaneous evacuation of stools, minimal soiling, independence of reservoir catheterization, and use of antidiarrheal drugs. The length of the efferent leg did not influence the function of the anal sphincter itself. The postoperative sexual life was unchanged, and all patients in group II had a better resocialization than those in group I. The selection of candidates for ileal reservoir operations from among patients with ulcerative colitis or familiar polyposis is most important because of a relatively long postoperative course and high incidence of surgical complications.

Adolescent↗
PubMed1983

Do intravenous urography and cystoscopy provide important information in otherwise healthy women with recurrent urinary tract infection?

An investigation of the influence on therapy of intravenous urography (IVU) and cystoscopy in a prospective study of 93 women with recurrent urinary tract infections is presented. The patients fulfilled the following criteria: a history of recurrent urinary tract infection (at least three episodes a year), a physical and gynaecological examination, determination of plasma creatinine, urine culture, microscopy, and uroflowmetry. It is concluded that IVU seldom reveals abnormalities that influence treatment in otherwise healthy women. On the other hand, uroflowmetry and cystoscopy occasionally yield helpful information.

Adolescent↗
PubMed1983

Acute complement activation during an anaphylactoid reaction to blood transfusion and the disappearance rate of C3c and C3d from the circulation.

A patient experienced an anaphylactoid shock after receiving a transfusion with 5-10 ml of leucocyte-depleted blood. Complement measurements revealed pronounced elevation of split products of the third complement factor (C3c and C3d), normal but fluctuating levels of native C3, significantly decreased levels of C4, normal factor B concentrations and low levels of factor B split products. The results indicate complement activation via the classical pathway as a causative mechanism of the anaphylactoid shock, and suggest an antigen-antibody reaction as the etiological factor. The nature of the antigen-antibody reaction initiating the complement activation could not be established. The disappearance rate (T/2) of C3d from the plasma after the acute C-activation was measured to approximately 4 hr, and the half life of C3c was significantly shorter.

Adult↗