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Biomedical subjects

L K Romanova

Publications and source records attributed to L K Romanova.

At least 19 recordsLinked to original sources
PubMed2000

Bronchoalveolar lavage cells and mitotic activity of monocytes and macrophages in rats after long-term intermittent hypoxia.

Long-term (1.5 months) intermittent hypoxia promoted desquamation of bronchial epithelial cells, decreased the relative content of alveolar macrophages and monocytes in bronchoalveolar lavage, stimulated lymphocyte migration to the lungs, and increased the relative content of lymphoid cell in rats. Mitotic activity of monocytes and macrophages decreased against the background of intraalveolar lymphocytosis (lymphocytic alveolitis).

Animals↗
PubMed1997

[The use of a new marker and test systems for the diagnosis of lung cancer with a study of bronchoalveolar washings].

Diagnostic value of enzyme immunoassays and immunoagglutination kits based on monoclonal antibodies ICO 25 was investigated. Antigen CAg 25 occurred in minimal quantities (5 micrograms/ml maximum) or was absent in the bronchoalveolar lavage of patients with bronchial asthma, chronic bronchitis or free of pulmonary affection. The concentration 9-19 micrograms/ml was registered in epidermoid bronchial cancer and peripheral lung cancer. It is stated that test systems able to detect a new antigen CAg 25 in BAL are diagnostically significant.

Agglutination Tests↗
PubMed1993

[Circadian fluctuations in the mitotic activity of the alveolar macrophages-monocytes from mouse bronchoalveolar washings].

The mitotic activity of alveolar macrophages-monocytes (AM-M) from bronchoalveolar lavages of 18 male (Balb x Black 6) mice F1 has been studied twice a day: at 8.00 a. m. (9 mice) and 8.00 p. m. (9 mice). Significant diurnal variations of AM-M proliferation were found. The mitotic index was 3.07 +/- 0.3/1000 in the morning and 1.39 +/- 0.3/1000 in the evening (p < 0.002).

Animals↗
PubMed1992

[Lymphocyte subpopulation state of the human lung during prenatal development].

Distribution of surface lymphocyte markers was assessed in foetus lung by cytofluorimetry using monoclonal antibodies produced by "Ortho Diagnostic systems" and "Becton Dickinson". Seven lymphocyte subpopulations were detected in developing lungs even at the pseudoglandular stage (8-15 weeks). There was a significant increase in these lymphocyte subpopulations in canalicular stage. Quantity of T3+ cells increased 8-fold and B-cells 10-fold. There was a tendency to increased number of cortical thymocytes (T6+), and to decreased ratio T6+/T3+ lymphocytes. The ratio of immunoregulatory lymphocyte subpopulations (T4+/T8+) resembled that of these subpopulations in adult human lung rather than in cord blood. In canalicular stage the share of lung lymphoid cells in S-phase was decreased.

Antibodies, Monoclonal↗
PubMed1992

[The clinico-cytological parallels in exacerbated bronchial asthma].

The authors provide the data on estimation of the character and intensity of inflammation in the proximal and distal bronchi as well as in the respiratory compartment of the lungs, obtained as a result of an all-round examination including a cytological assay of the bronchoalveolar washing off of 31 bronchial asthma patients in the period of disease exacerbation. A correlation analysis is given between the interrelationship between the intensity of bronchial obstruction and the level of eosinophilia in peripheral blood, sputum and in bronchoalveolar washing off. It has been revealed that the relative content of eosinophils in bronchoalveolar washing off is in the best agreement with the intensity of bronchial obstruction.

Adolescent↗
PubMed1991

[Physiologic regeneration of alveolar macrophages].

Alveolar macrophages (AM) play one of the leading roles in the modification of structure, function and behaviour of the respiratory organs cells. The size of AM population may determine the expression of the inflammatory and regenerative processes in the lung. Restoration of AM population may be brought about by two routes from monocytes coming to the alveoli from the circulation with subsequent differentiation into AM and as a result of local monocytes and macrophages proliferation. AM is a long-living cell population capable for limited self-maintenance and renewal.

Cell Count↗
PubMed1990

[Colony-forming hematopoietic cells in the mouse embryonic lung].

The colony-forming activity of embryo lung cells CBA mice was determined according to the Till and McCulloch technique (1961). After intravenous injection to jung cells (1 x 10(6)) from 14-day embryos the total number of colonies on the area of 1 mm2 of spleen sections from irradiated recipient mice averaged 2.31 +/- 0.39 whereas after transplantation of lung cells from 15-day embryos it averaged 2.34 +/- 0.53. The percent of macrocolonies equalled 52.5% in the former case and only 2.5% in the latter case. Macrocolonies contained cells of the myeloid, erythroid and megakaryocyte lines. Microcolonies predominantly consisted of granulocytes at various stages of differentiation. Thus, polypotent stem hematopoietic cells migrate into the fetal lung in vivo. The colony-forming ability of the lung polypotent stem cells decreases as the period of embryogenesis increases.

Animals↗
PubMed1990

[Complexities in the diagnosis of Pneumocystis pneumonia].

The authors provide clinical evidence for difficulties encountered in the diagnosis of pneumocystis pneumonia. Show the role of the methods such as examination of the bronchoalveolar lavage fluid and serological blood testing aimed at detection of the liter of antibodies to Pneumocystis carinii.

Adult↗
PubMed1990

[Difficulties in the diagnosis of chronic toxoplasmosis with lung involvement].

A case of chronic acquired toxoplasmosis combined with lung impairment is described. The diagnosis was made on the basis of the data obtained as a result of the use of the latest clinical and instrumental research methods and a specific serologic test. The diagnosis was verified morphologically on thin sections of the sputum. The authors provide data on the diagnostic search for approaches to the differentiation between parasitic diseases resulting in dissemination to the lungs with calcification of the foci and broncholithiasis, tuberculosis.

Animals↗
PubMed1989

Effect of colchicine and pilocarpine on the secretory activity of rat type II alveolar cells: an ultrastructural study.

Colchicine in a total dose of 0.6 mg/100 g body weight per day was shown to reduce the level of apical surfactant secretion by type II alveolar cells in random-bred male albino rats, thereby demonstrating that the cytoplasmic microtubules participate in the release of surfactant into the alveolar lumen. In addition, basal secretion of surface-active material was found in 51% of all the cells. In a single dose of 8 mg/100 g b.w., pilocarpine stimulated apical surfactant secretion. If injected after colchicine, it slightly increased the number of type II alveolar cells ready to release surfactant, but actual secretion was not observed; the level of basal secretion did not increase. It has been suggested that microtubular function is not completely responsible for basal secretion and is only partly responsible for apical surfactant secretion.

Animals↗
PubMed1988

[The cellular composition of the bronchoalveolar washing in experimental anthracosis].

Bronchoalveolar lavage (BAL) was performed in albino random-bred adult male rats with pneumoconiosis. Fibrotic reaction in the lungs was induced by inhalation coal dust during 6 months. BAL comprised 90.9 +/- 1.2% of macrophages; 4.4 +/- 0.8% of neutrophils and 4.5 +/- 1.0% of lymphocytes. The total cell number in BAL was higher (over 59%) in animals with pneumoconiosis. The number of cells on alveolar surface of the lungs was also higher. Fibrotic reaction in the lungs is probably directly related with the increased number of vital macrophages in the lung.

Animals↗
PubMed1988

[Mitotic activity of alveolar macrophages in nonspecific diffuse lung pathology].

Endopulmonary cytograms and mitotic activity of alveolar macrophages (AM) in the bronchoalveolar lavage (BAL) from 30 patients with diffuse lung pathology have been studied. AM mitosis was found in 24 cases. Mitotic index for all the patients was 0.65% and ranged from 0.25 to 2.75%. Electron microscopy revealed the capacity of differentiated forms of AM for mitotic division. Free AM may be regarded as slowly proliferating cell population, capable of self-reproduction in lung pathology.

Adult↗
PubMed1988

[Characteristics of the secretory activity of the respiratory cells of mouse lungs after partial chemical sympathectomy].

Partial "sympathectomy" in the neonatal BALB mice was achieved by the treatment with guanethidine. The number of neurons in the stellate ganglion decreased to 20% of the control values and remained constant throughout the subsequent period of 1 month. Partial "chemical sympathectomy" did not affect the postnatal growth and development of the lungs. Partial "chemical sympathectomy" significantly increased the number of secreting cells in bronchiolar and alveolar regions. Secretory activity of the alveolocyte population, type two, synthetizing and secreting surfactant also increased. It has been concluded that the partial "chemical sympathectomy" stimulated the alveolar surfactant secretion.

Animals↗
PubMed1988

[A case of alveolar microlithiasis].

A case of alveolar microlithiasis diagnosed on the basis of a clinical and x-ray picture was described. The diagnostic value of an endopulmonary cytogram in this patient was discussed. The authors investigated bronchoalveolar washing off obtained from bronchoalveolar lavage for morphological diagnosis of this pathology.

Adult↗
PubMed1987

[Characteristics of the intrapulmonary cytogram in compensatory lung hypertrophy].

Bronchoalveolar lavage (BAL) was performed on days 1, 3, 5 after left-side extirpation of the lung in albino random-bred adult male rats. BAL cell composition was studied. Compensatory lung growth was shown to be accompanied by restoration of BAL total cell number. BAL comprised 81.7% of macrophages, 15.6% of neutrophils and 2.5% of lymphocytes. The number of neutrophils was high (over 20%) in animals with postoperative pneumonia.

Animals↗
PubMed1984

[Respiratory portion of the lungs of intact BALB strain mice].

Four male mice (1-month-old) of BALB strain have been studied. Three types of cells participate in formation of cellular lining of the terminal branchioles: ciliated, Clara cells and villose cells which are included in the neuroepithelial bodies composition. Clara cells are numerous and they are similar in their ultrastructural organization, they contain a great number of polymorphous secretory granules, single mitochondria and a poorly developed endoplasmic reticulum. A comparatively large number of pores of Khone are revealed: there are 4-8 pores per one alveole, and one pore is situated on 197.4 mcm2 of the alveolar surface. Alveolocytes of the second type of make the main actively secreting cell population of the alveolar passages and alveoles. Simultaneously, at the state of secretion there are 76% of the cells. The secretion of alveolar surfactant is realized by means of merocrine exocytosis. Relative volumetric fraction of mitochondria in alveolocytes of the II type comprises 12% and that of "cytophospholiposomes" (osmiophilic laminar bodies)--11%.

Age Factors↗