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Biomedical subjects

L Kilham

Publications and source records attributed to L Kilham.

At least 37 records · Page 2Linked to original sources
PubMed1975

Encephalitis in suckling rats induced with rat cytomegalovirus.

Encephalitis has been induced in suckling rats by intracerebral inoculation of rat cytomegalovirus. This agent has been carried through five animal passages without changes in virulence. Principal sites of viral attack were the meninges and ependyma with limited parenchymal invasion from these sites. Characteristic features of cytomegalovirus disease observed included cytomegaly, formation of nuclear and cytoplasmic inclusions, and polykaryocytosis. Fundamental tinctorial and optical differences were noted between nuclear and cytoplasmic inclusions, the latter being characterized by their phloxinophilia which contrasts with the hematoxylinophilia of nuclear inclusions. Differential interference microscopy improved visualization and photography of cytoplasmic inclusions. Hydrocephalus developed in a few of the animals and was studied in late phases of the disease.

Animals↗
PubMed1974

Experimental adenovirus infection of the mouse adrenal gland. I. Light microscopic observations.

A murine type of adenovirus has been found to have a highly selective affinity for the adrenal of its natural host. In view of the adrenal pathology in Addison's disease, which consists of cortical atrophy and focal lymphocytic infiltration, the possibility of a viral causation is an attractive hypothesis. The striking adrenotropism of this agent offers, theoretically, a potential experimental animal model for investigation of this possibility. Most of our information about the acute biologic action of adenoviruses has been gained from study of virus-infected tissue culture cells. The sharply focused and severe attack of the murine agent upon a prime target organ facilitates correlative in vivo studies of the cytopathology of adenovirus infection at levels of light and electron microscopy.

Adenoviridae Infections↗
PubMed1974

Experimental adenovirus infection of the mouse adrenal gland. II. Electron microscopic observations.

A strain of mouse adenovirus, found to have a striking tropism for the weanling mouse adrenal gland, enabled electron microscopic examination of adrenals in various stages of infection. Nucleolar hypertrophy and the successive formation of three types of inclusion bodies in association with nucleoli preceded virion production. Angular crystals of virions formed in the affected nuclei. Virus was released by lysis of nuclear membranes; rapid degeneration of cytoplasmic organelles followed. Rupture of external cell membranes released virus into the extracellular spaces where virions crossed vascular basement membranes to enter endothelial cells. Virions were also phagocytized by inflammatory cells which reentered vascular sinusoids, and by adrenal parenchymal cells. Disruption of virus-laden phagocytic vacuoles in parenchymal cells released virions into the cytoplasm. Typical viral inclusion bodies also formed in vascular endothelial cells and in inflammatory cells, but virion replication was not detected. The possibility that virus directly entered parenchymal cells through the external cell membrane without phagocytosis is discussed.

Adenoviridae Infections↗