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Biomedical subjects

L Korngold

Publications and source records attributed to L Korngold.

At least 19 recordsLinked to original sources
PubMed1982

Association of anti-F (ab')2 antibodies (pepsin agglutinators) with immune complexes as determined by enzyme-linked immunosorbent assays.

A solid-phase assay for the detection of anti-F(ab')2 antibodies is described. Wells of microtiter plates are coated with F(ab')2, dilutions of sera are added, and IgG bound to the solid phase is detected using peroxidase-labeled Protein A. Anti-F(ab')2 antibodies were found in 61% of sera from patients with rheumatoid arthritis, 77% with subacute bacterial endocarditis , and 34% with systemic lupus erythematosus. Simultaneous analysis of these sera for immune complexes (IC), using modified Clq and monoclonal rheumatoid factor assays, showed that a correlation existed between anti-F(ab')2 antibodies and the levels of IC. Characterization of anti-F(ab')2 antibodies by inhibition and absorption experiments and by immunological and physical means indicated that they were similar to serum proteins described in the 1960s and designated pepsin agglutinators.

Antibodies, Anti-Idiotypic↗
PubMed1981

A solid-phase assay for circulating immune complexes using monoclonal rheumatoid factors and peroxidase-linked protein A.

A solid-phase monoclonal rheumatoid factor (mRF) assay for circulating immune complexes (IC) is described. Microtiter plates are coated with mRF, serum diluted 1 : 40 is added, and complexed IgG is detected with protein A coupled to peroxidase. Selective binding was obtained with aggregated human gamma-globulin and soluble IC prepared in vitro. Sucrose density gradient ultracentrifugation studies indicate that mRF binds intermediate-size IC (between 7S and 19S). Abnormal levels of IC were found in 78% of sera from patients with rheumatoid arthritis, 67% with systemic lupus erythematosus, and 87% with subacute bacterial endocarditis.

Antigen-Antibody Complex↗
PubMed1980

Structural heterogeneity in p30 molecules of type C viruses.

Tryptic digests of p30 proteins from mouse type C viruses were subjected to cation-exchange chromatography. Structural heterogeneity of p30 molecules was seen in two specific areas of the peptide elution profiles. These hypervariable regions of p30 proteins were used to discriminate representative ecotropic (N- and B-tropic), xenotropic (alpha and beta) and amphotropic viruses.

Animals↗
PubMed1979

Tryptic peptide analysis of gag gene proteins of endogenous mouse type C viruses.

Tryptic digests of the internal proteins p30, p15, p12, and p10 of mouse xenotropic, ecotropic, and amphotropic type C viruses were subjected to cation-exchange chromatography. Analysis of these maps revealed that the p30 proteins from representative isolates of all three viral subgroups were distinguishable. The p15 proteins were all unique. The p12 proteins of NZB xenotropic and wild-mouse amphotropic viruses were not identical and yielded peptide maps remarkably different from that of the ecotropic virus. The p10 proteins of xenotropic and ecotropic viruses were identical and were dissimilar to that of the wild-mouse amphotropic virus.

Animals↗
PubMed1977

Further characterization of alkaline phosphatases of human kidney and urine.

Human kidney alkaline phosphatase (AP) react with antisera to liver and intestinal AP. The respective kidney enzymes are designated as KLi and KI. Comparison with liver AP showed that KLi was salted out at the same ammonium sulfate concentration (66%) as the liver enzyme; KLi was antigenically identical with liver AP; the electrophoretic mobility of KLi was that of a gamma-globulin whereas liver AP was an alpha2-globulin. the liver enzyme was lighter (130,000 daltons) than KLi (150,000 daltons). KI and intestinal AP shared most properties: antigenically they were indistinguishable; both contained two components one of which was 80,000 daltons, the other was heavier (120,000 daltons for KI, 130,000 daltons for intestinal AP). Urinary AP consisted only of the lighter component (80,000 dalton). Methods for partial separation of the three kidney APs are given.

Alkaline Phosphatase↗
PubMed1976

The alkaline phosphatases of human liver: an immunochemical study.

Four out of 18 human liver alkaline phosphatase (AP) preparations reacted with anti-liver and anti-intestinal AP. The liver AP that reacted with anti-intestinal AP, designated as intestine-like AP, was precipitated at 50% ammonium sulfate whereas the major liver AP precipitated at 66% saturation. Gel filtration showed that liver AP, intestine-like AP and intestinal AP contained AP with apparent molecular weights of 130,000 daltons; the intestine-like AP contained a second but smaller component of 70,000-80,000 daltons. AP extracted from intestine also contained this smaller component; its electrophoretic mobility was that of an a2-globulin, whereas that of the intestine-like AP had a mobility of beta-globulin. The similarity of the intestine-like AP to the AP variant found in hepatomas is stressed.

Alkaline Phosphatase↗
PubMed1975

Observations on trace proteins in plasma of febrile patients by cationic disc electrophoresis in acrylamide gel at pH 3.8.

Cationic disc electrophoresis at pH 3.5 in 6 M urea-containing acrylamide gels permits analysis of plasma and other body fluids for the presence of trace proteins with pI greater than or equal 5 and M.W. less than 60,000. These charge and size characteristics would include rabbit and human granulocytic pyrogen, human monocytic pyrogen and, by inference, other similar candidate pyrogenic proteins. Semiquantitation of the trace protein content can be achieved by densitometric scanning of gels stained with Amido schwarz. Duplicate analyses were performed on plasma samples from 133 individuals: normal, 15; afebrile advanced cancer, 18; afebrile Hodgkin's disease, 30; febrile Hodgkin's disease, 33; other febrile lymphoma, 13; febrile advanced cancer without infection, 12; and pyogenic fever, 12. Plasma from most of the febrile patients, particularly from febrile Hodgkin's disease patients, contained trace proteins not detectable in the afebrile individuals studied. In patients with Hodgkin's disease the quantity of trace proteins present in plasma correlated well with overall severity of Hodgkin's pyrexia, but not with spontaneous hr to hr fluctuations in the fever. Marked reduction in plasma levels of the trace proteins occurred with response to antitumor therapy. Elevated plasma levels of these proteins can be induced by intratumoral inoculation of Bacillus Calmette-Guérin. They appear concomitant with the febrile response.

BCG Vaccine↗