Biomedical subjects
L Kubes
Publications and source records attributed to L Kubes.
Experimental immune complex glomerulonephritis in the mouse with two types of immune complexes.
Immune complex glomerulonephritis was induced in three groups of mice by long-term immunization. Two antigens of similar molecular weight were used. The first group was immunized with ferritin (mol wt 480,000). In altered glomeruli deposits of immune complexes were seen in the subendothelial and subepithelial spaces of the glomerular basement membrane (GBM) and in the mesangium. The immune complex deposits were formed by amorphous matrix with marked dense molecules of ferritin. The second group was immunized with human fibrinogen (mol wt 450,000). The immune complex deposits were present in the intramembranous, subepithelial and subendothelial spaces of the GBM and in the mesangium. These deposits were relatively less electron-dense and had a fine granular structure. The third group of mice were immunized with both ferritin and fibrinogen simultaneously. Two types of deposits situated subendothelially in the GBM and in the mesangium were seen in one animal of this group. One type of deposit resembled structurally the ferritin-antiferritin complex deposits, the other resembled the fibrinogen-antifibrinogen complex deposits. The individual deposits in the GBM and in the mesangium formed discrete homogeneous masses. The two types of deposit were occassionally in direct contact with one another, but were more often completely separate and were never mixed. It can be assumed that in at least some phase of the experiment both types of complex were present in the circulating blood simultaneously. However, since none of the complexes deposited in the GBM or in the mesangium were mixed, it seems probable that each type of complex is deposited separately in the form of "clusters" composed of a single type of complex. The phagocytic activity of mesangial cells of animals with complex glomerulonephritis was not increased when compared with control animals.
[IgA nephropathy].
A series of 150 samples of renal tissue obtained by needle biopsy from patients with haematuria were examined immunohistologically. In 15 cases mesangial deposits of IgA were demonstrated. In most cases they were young patients in whom no hypertension, oedema or laboratory findings indicating renal damage could be detected one year later during a follow-up examination. Electron microscopical examination was carried out in the renal tissue of 7 such cases, and showed dense mesangial deposits and a marked subendothelial rarefaction within the basement membrane. The light microscopical findings were variable. Three cases showed minimal lesions, 5 mesangial proliferative glomerulonephritis and 7 mesangial proliferative glomerulonephritis with focal segmental sclerotic alterations within the glomeruli.
[Malignant nesidioma with 2 types of secretory granules in tumor cells].
A case has been described of a male aged 38 with a malignant nesidioma. The clinical examination disclosed marked hepatomegaly, variable hypertension associated with cool sweating, agitation and transient hyperglycaemia. The primary tumour as well as the metastases in the liver weighing over 13 kg were histologically of trabecular structure and stained positively with the methods of Bodian and grimelius. The impregnation for D-cells according to Rohoznická and Vortel was positive in isolated cells only. Ultrastructurally, the tumor cells could be divided in two types. The more numerous Type I cells contained rather small secretory granules morphologically resembling A granules of islet cells, and staining positively with impregnation methods for A-cells on the ultrastructural level. Type II cells contained, in addition to the formerly described ones, another type of granules appearing as large, slightly osmiophilic ones characteristic of the D-cells of Langerhan's islets and positive with the impregnation methods for D-cells. The type II tumour cells must be therefore regarded as a mixed cell type containing two types of secretory granules.
Gomori-positive cells in rat thymus: Lack of evidence on endocrine function.
Large Gomori-positive cells were observed in rat thymus after hormonal induction (e. g. after treatment with oxytocin or hydrocortisone). In contrast to some findings by others, no evidence on endocrine function of these cells was found and they were identified as macrophages containing, in their cytoplasm, great amount of phagocytized material.