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L Kuehl

Publications and source records attributed to L Kuehl.

24 records · Page 2Linked to original sources
PubMed1971

Studies on the site of synthesis of several soluble enzymes of the cell nucleus.

Rats were given radioactive L-leucine intravenously. At various times after injection, the livers were removed and separated into nuclear and cytoplasmic fractions by a nonaqueous technique. Glyceraldehyde-3-phosphate dehydrogenase, aldolase, and lactic dehydrogenase were isolated from each cell fraction by antibody precipitation followed by gel electrophoresis, and the specific radioactivities of the isolated enzymes were determined. In all three cases, the onset of labeling and the rate of incorporation were the same for the nuclear enzyme as for the corresponding enzyme from the cytoplasm. If we assume that equilibration of the enzymes between the cytoplasmic and nuclear pools occurs slowly relative to the labeling times employed, we may conclude that the labeled nuclear enzymes either were synthesized in the nucleus or moved into the nucleus from a cytoplasmic site of synthesis without first passing into the cytoplasmic pool of enzyme. Treatment with puromycin, an antibiotic which depresses incorporation into cytoplasmic proteins to a greater extent than into nuclear proteins, led to a situation in which the specific activities of the nuclear enzymes were several times as high as those of the corresponding cytoplasmic enzymes following a short period of incorporation. These data substantiate the assumption that equilibration between the cytoplasmic and nuclear enzyme pools occurs slowly and provide further evidence that the labeled nuclear enzymes do not arise from the cytoplasmic enzyme pool.

Animals↗
PubMedDate not supplied

Open-ended vasectomy: approaching the ideal technique.

BACKGROUND: This study was conducted to determine whether the open-ended technique of vasectomy is an improvement over traditional closed-ended techniques. METHODS: A switch from closed-ended to open-ended vasectomy was affected in 1988 at the authors' vasectomy clinic. Patients were contacted by telephone 1 to 3 years after vasectomy. RESULTS: The authors successfully contacted 200 of 257 consecutive open-ended vasectomy patients (78 percent). Among the 200 men there were no reported pregnancies among their partners, but there was one (0.5 percent) failure of the sperm to clear, which was treated by repeat vasectomy. There were 3 (1.5 percent) mild infections, 1 (0.5 percent) sperm granuloma, and 1 (0.5 percent) case of late, intermittent pain. CONCLUSIONS: This open-ended vasectomy series has low complication and failure rates, corroborating findings from two larger series. There is no increase in the failure rate using the open-ended technique compared with the closed-ended technique. The single case of late pain is consistent with a decrease in this complication. Open-ended vasectomy approaches the ideal vasectomy.

Humans↗