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L Kunzová

Publications and source records attributed to L Kunzová.

At least 19 recordsLinked to original sources
PubMed1991

Studies of natural population variability of parainfluenza viruses during their epidemic circulation.

The population of circulating serotype 3 parainfluenza virus strains isolated in different years proved to be sufficiently polymorphic concerning its antigenic and biological features as well as their virulence for newborn hamsters. The highly virulent strain population appeared to have an antigenic pattern different from that of the prototype strain. The epidemic caused by it in groups of school and preschool children was more intensive as compared to that induced by avirulent strains population.

Animals↗
PubMed1990

[Surveillance of acute respiratory diseases in the 1988/1989 season in Czechoslovakia].

The authors submit an analysis of results of surveillance of acute respiratory diseases during the 1988/1989 season in the CSSR. The influenza epidemic took place between the 4th and 11th calendar week with a peak in the 7th week. During the epidemic 19.7% of the population in the CSR contracted the disease. The influenza epidemic was preceded by a high incidence of infections caused by a respiratory syncytia virus. As to the aetiology, influenza viruses subtypes A/H1N1/, A/H3N2/ and type B participated practically equally in the epidemic. The authors discuss the observed changes in the influenza epidemiology in recent years in Czechoslovakia and neighbouring countries.

Acute Disease↗
PubMed1990

Epidemiological analysis of acute respiratory disease (ARD) and characteristics of the influenza epidemic in Bohemia during the season 1986/1987.

The authors analyze the findings of epidemiological and virological surveillance of ARD in Bohemia during the season 1986/1987. In all, 57.5% of the Czech population was affected by acute respiratory disease (ARD). There were 5,950,832 cases reported, 124,444 complications (2.1% of the overall morbidity rate) and 5,374 deaths due to influenza, bronchitis, pneumonia and chronic pulmonary affection. The influenza epidemic commenced during the 48-th calendary week (CW) and lasted 5 weeks till the 52-nd CW. The epidemic was due to an influenza virus strain of the subtype A(H1N1) antigenically related to the drift variant A (Singapore) 6/86. Within an extremely short period of the epidemic, 1,094,865 influenza cases were reported and 22,313 cases of complications. 10.7% of the CSR population were affected during the epidemic in whose etiology noninfluenza respiratory viruses were significantly implicated, especially adenoviruses (41.7%) and the RS virus (26.9%). There was no excessive mortality in the course of the epidemic. The authors discuss the atypical nature of this particular influenza epidemic and the etiological role of respiratory viruses.

Acute Disease↗
PubMed1989

Use of nasal and pharyngeal swabs for rapid detection of respiratory syncytial virus and adenovirus antigens by enzyme-linked immunosorbent assay.

Nasal and pharyngeal swabs from 134 children with acute respiratory diseases were examined for the presence of respiratory syncytial (RS) virus and adenovirus antigens by enzyme-linked immunosorbent assay (ELISA). The results were compared with those obtained by virus isolation and serology. Altogether, 56 RS virus-positive (prospective study), 51 adenovirus-positive (retrospective study), and 27 negative (prospective study) samples were examined. The sensitivities of ELISA were 96 and 98% for RS virus and adenovirus antigen detection, respectively. No false-positive results were observed. It is concluded that the joint eluate from a nasal and a pharyngeal swab, properly taken and handled, serves well for the detection of RS virus and adenovirus acute respiratory infections by ELISA.

Adenovirus Infections, Human↗
PubMed1989

[A tonsillitis epidemic caused by adenoviruses].

An epidemic of lacunar angina which affected, in relatively close common life, mostly only one of nine military units living in the same area. The failure of penicillin and negative finding of streptococci resulted in a retrospective establishment of the agent, which with all probability was adenovirus type 4. It has become obvious that the typical picture of lacunar or even pseudomembranous angina is not a reliable sign indicating streptococcal etiological even in a mass infection. The authors draw attention to the importance of continuous bacteriological surveillance even if only several cases of pharnygotonsillitis occur in a community. Uncertainties of the use of penicillin are then unnecessary in the therapy and even prophylaxis of the whole group, even if the first view may suggest its use. Some epidemiological features of the mass adenovirus angina are discussed.

Adenoviridae Infections↗
PubMed1989

[Epidemic keratoconjunctivitis. Laboratory study of patients infected with adenovirus type 8].

The authors tried to detect, using different methods, the causal agent of the disease in eight patients with epidemic keratoconjunctivitis. From the conjunctival sacs of one ambulatory patient and six of seven patients hospitalized on account of other eye diseases they isolated 11 strains of adenovirus type 8 on cell cultures from human embryonic lungs. Using direct and indirect ELISA, they detected adenovirus antigen in four of seven examined smears from the conjunctival sac without previous cultivation. In smears from the nasopharynx they did not detect adenoviruses by either of the two methods. Antibodies were assessed by micromodifications of the virus neutralizing test, by the complement fixation reaction and ELISA. A significant rise of antibodies against adenovirus type 8 and adenovirus hexone resp. was found in different six of eight examined pairs of sera. The combination of these three serological methods made it possible to confirm adenovirus infection in all eight patients.

Adenoviridae Infections↗
PubMed1989

Occurrence and aetiology of acute respiratory diseases: results of a longterm surveillance programme.

Totals of 58,661,000 acute respiratory disease (ARD) cases, 1,376,651 bronchitis and pneumonia complications, and 93,042 deaths from influenza, bronchitis, pneumonia or chronic pulmonary affection were notified during 11 years of ARD surveillance from 1975 to 1986. All ARD seasons started with the first phase in September-December; this increase in morbidity was caused chiefly by adenoviruses, parainfluenza viruses, rhinoviruses and M. pneumoniae. Second wave of ARD morbidity occurring in January-April used to be explosive and was associated with an influenza epidemic in 9 of the 11 seasons; only in 1978/79 and 1984/85 the ARD epidemics were caused by adenoviruses and especially RSV, the share of influenza being minimal. Pneumonia and bronchitis excesses occured during epidemics caused by M. pneumoniae in 1975/76, 1980/81 and 1985/86. Particularly high mortality excesses occurred in 1976, 1977 and 1983 during epidemics elicited by a new drift variants of influenza A(H3N2). Identification of viral agent of M. pneumoniae attempted in 5474 ARD cases was successful at 37.4%. The respective contributions of parainfluenza viruses, adenoviruses, influenza A virus and RSV to overall aetiologically identified morbidity were 14.2, 13.9, 13.8, and 12.0%. Mixed infections (2-3 agents identified simultaneously) accounted for 14.6% of cases. Type B influenza virus, rhinoviruses, enteroviruses and herpes simplex virus contributed only by 5.6-7.8%. In ordinary seasons the share of M. pneumoniae in aetiologically identified ARD morbidity was 0.6-3.8%; this agent displayed predominance at 5-year cycles, when accounting for 20.5-38.9% of cases. The most frequently detected agents in individual age groups were as follows: in preschool children parainfluenza (18.6%), RSV (16.6%), and adenoviruses (17.4%); in school children M. pneumoniae (26%), influenza A and B (10.2 and 14.7% respectively), and adenoviruses (10.7%); in adolescents and young adults influenza type A (20.2%), M. pneumoniae (15.0%), and rhinoviruses (13.3%); in adults above 25 years age influenza A virus (38%), and other respiratory viruses at a frequency lower than 10% each.

Adolescent↗
PubMed1989

[Specific and nonspecific indicators of activation of influenza viruses before an epidemic caused by influenza viruses subtype A/H3N2 and type B in Czechoslovakia in 1986].

The authors submit an aetiological and epidemiological analysis of the influenza epidemic which occurred in the CSR between the 4th and 14th week of 1986 and was caused by the influenza virus subtype A/H3N2/ and type B. The epidemic affected a total of 27.1% of the population, in the age group of 0-5 years 63.7%, in the age group 6-14 years 52.7% and in the age group above 15 years 17.1%. In the course of the epidemic 77,458 cases of pneumonia and bronchitis were reported and 1,412 deaths with the diagnosis influenza, bronchitis, pneumonia and chronic affection of the lungs. The authors analyze also specific indicators of the activation of influenza viruses and reach the conclusion that serological evidence of the circulation of influenza viruses in the population was detected already in the third quarter of 1985, the first isolations were made six weeks before the influenza epidemic. Activation of the influenza viruses is indicated already during the pre-epidemic period by some non-specific indicators which include the rising number of patients with acute respiratory affections in surgeries and the rising number of children absent from nurseries and nursery schools on account of these diseases. The most sensitive non-specific indicator is the rising number of patients with respiratory diseases in surgeries of the First aid medical service.

Adolescent↗
PubMed1980

An outbreak of respiratory disease due to a type 5 adenovirus identified as genome type 5a.

A cluster of adenovirus type 5-caused acute respiratory diseases was observed in Prague in the autumn-winter season of 1978/79. An analysis of viral DNA by sequence-specific restriction enzymes in two out of 39 isolates revealed that these isolates, identical to each other, were clearly distinct from adenovirus type 5 prototype strain. The designation "adenovirus genome type 5a" is suggested for these isolates.

Adenoviridae Infections↗
PubMed1979

Incidence of RS virus infections in premature children's ward.

In the course of two years (1974-76) four outbreaks of acute respiratory disease in the premature children's ward of a Prague hospital were studied virologically and clinically. RS virus (RSV) was found to be the aetiological agent. The highest isolation rate of RSV was achieved when using two heteroploid cell lines (L-132 and HEp-2 cells) simultaneously. Of the 30 children examined, 60% showed a severe course of disease (pneumonia and/or bronchiolitis) while in 40% of the children the disease had the form of rhinitis with striking abundance of whitish foamy secretions. In one of the outbreaks under study, two nurses with mild afebrile pharyngitis were detected as the source of RSV infection.

Bronchiolitis, Viral↗
PubMed1976

Influenza and other actue respiratory diseases in the Czech Socialist Republic, 1969-1974.

Since 1969, the incidence of acute respiratory diseases (ARD) in the Czech Socialist Republic of Czechoslovakia has been monitored by a special programme based on reports from 85 district epidemiological centres. In this paper, the incidence of ARD in three age groups, together with the incidence of complications and death rates, are presented for each season during the period 1969-1974. The significance of epidemiological observations and laboratory investigations relating to influenza and other ARD agents, such as parainfluenza viruses, adenoviruses, rhinoviruses, RS virus, coronaviruses, and Mycoplasma pneumoniae, is discussed.

Adolescent↗