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Biomedical subjects

L L Berger

Publications and source records attributed to L L Berger.

At least 37 records · Page 2Linked to original sources

Spent cellulose casings as potential feed ingredients for ruminants.

Cellulose casings are used to contain and form meat and poultry emulsions during the smoking and cooking process. Casings then are stripped from the cooked product and traditionally disposed of in landfills. Because of the bulk of the spent cellulose casings (SCC), rapid composting technology may be used to reduce bulkiness. The following SCC were evaluated in vitro and in vivo: fibrous ground (FG), fibrous composted (FC), NOJAX ground (NG), and NOJAX composted (NC). In vitro digestibility was determined by incubating SCC with mixed ruminal bacteria for 0, 6, 12, 24, 36, 48, and 72 h. In vivo data were collected using four ruminally cannulated Holstein steers in a 4 x 4 Latin square design. Diets consisted of a 50:50 ratio of alfalfa hay-wheat middlings with 5% cornsteep liquor. Diets contained no SCC (CON) or 25% (DM basis) of the FC, FG, or NC SCC substrate. Casings were high in structural carbohydrate and salt content but low in CP, ether extract, and lignin concentrations. In vitro OM digestibility at 24 h was highest (P < .05) for FC and lowest (P < .05) for NG; FG and NC were intermediate. Composting tended to reduce fiber content and increase digestion. In vivo intakes and digestibilities were not adversely affected by inclusion of SCC in the diet. Thus, SCC have the ability to partially replace more traditional forages, such as alfalfa hay and wheat middlings, in high-fiber diets for growing beef cattle. Limitations in the use of SCC as a partial replacement of traditional feedstuffs will likely be because of high salt concentrations in the casings resulting from product brine chilling.

Animal Feed↗

Effects of prenatal androgenization, melengestrol acetate, and Synovex-H on feedlot performance, carcass, and sensory traits of once-calved heifers.

Three studies were designed using a 2 x 2 factorial arrangement of treatments within each experiment to evaluate the effects of various management techniques on growth and carcass traits of once-calved heifers. In Exp. 1, 33 yearling (16 control [C] and 17 prenatally androgenized [PA]) and 39 once-calved (20 C and 19 PA) nonpregnant heifers were used to evaluate the effects of age and PA. Once-calved heifers were approximately 31 kg heavier (P < .03) on-test, initially consumed 11% more (P < .01) DM, and gained 11% faster (P < .08), but had similar (P = .44) gain to feed ratios (.138 vs. .131) as yearlings. Growth performance was not affected by PA. The yearling and once-calved heifers were then slaughtered at approximately 707 and 1,080 d of age, respectively. Despite maturity differences (P < .01), marbling scores (P = .26) and percentage of choice carcasses (P = .27) were similar. However, on the basis of newly accepted grading changes, percentage of once-calved heifer carcasses grading choice decreased from 57.3 to 34.4%. Organoleptic properties did not differ. Experiments 2 and 3 were then conducted to evaluate effects of commercially available products (melengestrol acetate [MGA] and Synovex-H) on feedlot characteristics of heifers following weaning of their first calf. In Exp. 2, 48 once-calved Simmental-based heifers were fed during the summer to a constant compositional fat thickness of .8 cm. Experiment 3 was conducted in the late fall and early winter, during which 52 moderately sized heifers were fed for 116 d postweaning and slaughtered. Performance of heifers in Exp. 2 was not affected by MGA. However, heifers fed MGA in Exp. 3 gained 9% faster (P < .05) than controls. In Exp. 2, Synovex-H tended to improve ADG (P < .09) and feed efficiency (P < .07), but did not affect performance in Exp. 3. Most carcass traits were not affected by either treatment. Nonpregnant, once-calved heifers seem to have a large capacity for efficient weight gain as compared with yearling heifers. However, hormonal compounds, such as testosterone propionate provided pre- or postnatally (Synovex-H), and MGA do not appear to provide the consistent, positive responses often seen with younger, more typical feedlot heifers.

Aging↗

Effects of feed intake and dietary level of wet corn gluten feed on feedlot performance, digestibility of nutrients, and carcass characteristics of growing-finishing beef heifers.

The objective was to compare the relative energy value of wet corn gluten feed (WCGF) to that of corn in a feedlot situation when diets were initially offered ad libitum (AL) or at restricted feed intake (RFI; 80% of AL). In a completely randomized block (pen location) design experiment, 144 beef heifers (204.1 +/- 18.2 kg) were used (six treatments; three pens of eight heifers/treatment). Treatments were levels of WCGF (on DM basis) in corn silage-based diets (AL; 25 or 50% WCGF) or high-moisture corn-based diets (RFI; 0, 25, 50, or 75% WCGF) during the growing phase (127 d). During finishing (84 d), all diets were offered AL and contained 5% corn silage by replacing corn silage with high-moisture corn in diets that were offered AL. Heifers that were initially at AL had similar (P > .1) feedlot performance (during growing and during the whole trial), digestibility of nutrients (OM, NDF, CP, and GE), and carcass characteristics. During finishing, however, these heifers had better (P = .06) ADG and gain:feed when 25% WCGF was fed. Heifers that were initially at RFI showed a linear decrease (P < .01) in ADG and gain:feed during growing with increasing dietary level of WCGF. However, increasing dietary level of WCGF resulted in a quadratic (P = .02) response in ADG and gain:feed during finishing and also in a quadratic (P = .07) response in ADG and a linear (P = .005) decrease in gain:feed during the whole trial. These quadratic responses indicated that the best performance was achieved at the 25 and 50% levels of WCGF. The decrease in cumulative gain:feed was only 3.5% at the 25 and 50% levels of WCGF but it was 11.4% at the 75% level. Increasing the level of WCGF in diets of heifers that were initially at restricted feed intake did not affect (P > .1) digestibility of nutrients but it improved some carcass characteristics linearly, decreasing fat thickness (P = .04), liver abscess (P = .02), and yield grade (P = .13). Results suggest that WCGF can substitute up to 25 or 50% of dietary DM without negative effects on feedlot performance, digestibility of nutrients, or carcass characteristics. In addition, restricting feeding during growing may be strategy that improves the utilization of WCGF at these levels.

Animal Feed↗

Feedlot performance and carcass characteristics of Holstein steers as affected by source of dietary protein and level of ruminally protected lysine and methionine.

The objective of this study was to determine the effects of source of dietary CP and level of ruminally protected lysine and methionine (RPLM) on feedlot performance and carcass characteristics of Holstein steers during a growing-finishing trial (266 d). A total of 168 Holstein steers (182.7 +/- 27.5 kg) were used in a completely randomized design experiment (eight treatments; three pens of seven steers/treatment). Steers were given ad libitum access to high-concentrate diets (13% CP) containing 71% whole shelled corn, 10% corn silage, 4% condensed distillers solubles, and 15% protein supplements (DM basis). Treatments were arranged as a 2 x 4 factorial. The main factors were two sources of dietary CP and four levels of RPLM. The sources of dietary CP were soybean meal (SBM) or SBM and urea (SBM-U). Urea-N replaced 50% of SBM-N in the SBM-U diet. The levels of RPLM were 0, 5, 10, and 15 g per steer daily. No interactions (P > .10) between source of dietary CP and level of RPLM were observed for feedlot performance or carcass characteristics. Feedlot performance showed an advantage (P < .10) to feeding SMB during the first 84 d of the trial and an advantage to feeding SBM-U during the last 98 d of the trial. However, feedlot performance for the whole trial and carcass characteristics (except for fat thickness) were not affected (P > .10) by the source of dietary CP. Steers fed diets containing SBM-U had 12% less (P < .10) fat thickness than those fed diets containing SBM. Supplementation of diets with increasing levels of RPLM did not affect (P > .10) ADG or carcass characteristics. However, DMI and gain:feed showed cubic (P < .10) responses to increasing dietary level of RPLM. Supplementation of RPLM at the 10 g/d level improved gain:feed by 12% during the last 98 d of the trial, and this was a direct response to the cubic effects of RPLM on DMI. Results suggest a cost advantage for replacing 50% of SBM-N with that from urea in high-corn diets without negative effects on feedlot performance or carcass characteristics of growing-finishing Holstein steers with extended feeding periods (266 d). These types of diets seem to meet the amino acid requirements and are not limiting in lysine and methionine.

Animal Feed↗

Parturition and periparturient reproductive and metabolic hormone concentrations in prenatally androgenized beef heifers.

Primiparous Angus x Simmental heifers (n = 43) in a single-calf heifer (SCH) system (i.e., heifers are bred, calved, and placed in drylot pens with their calves at side and fed to slaughter weights) were studied to evaluate the effects of prenatal androgenization on parturition and on periparturient reproductive and metabolic hormone concentrations. Seven prenatally androgenized (PA) and seven control (C) heifers were used for blood collection to characterize parturient and lactation endocrine profiles; all heifers were used for blood collection to characterize postpartum ovarian cyclicity. Serum concentrations of progesterone, estradiol, testosterone, 13,14-dihydro-15-keto-PGF2 alpha (PGFM), and prolactin from 10 d before to 3 d after parturition were similar for PA and C heifers. Calf birth weights (34.7 +/- .9 kg) and calving ease scores (1.34 +/- .14) were similar between treatments. Postpartum ovarian cyclicity was similar; only 6 of 22 PA (27.3%) and 3 of 21 C (14.3%) heifers were cyclic by 70 d postpartum, based on weekly serum progesterone concentrations. Serum concentrations of insulin, triiodothyronine (T3), and prolactin at 35, 70, and 105 d of lactation were similar for PA and C heifers; thyroxine (T4) concentrations were similar at 35 and 70 d but greater (P < .01) at 105 d of lactation in PA heifers than in C heifers. Although mean serum concentrations of insulin, T3, and T4 were similar between treatments, prolactin concentrations were greater (P < .05) in C than in PA lactating heifers. We conclude that PA heifers are similar to C heifers with respect to parturition and to periparturient reproductive and metabolic hormone concentrations. Therefore, management requirements of PA primiparous beef heifers seem to be similar to those of C primiparous beef heifers, and PA heifers can be used successfully in a SCH system.

Androgens↗

Effect of roasting on site and extent of digestion of soybean meal by sheep: I. Digestion of nitrogen and amino acids.

Six mature wethers (BW 72 kg) with ruminal, duodenal, and ileal cannulas were fed a control (no added soybean meal [SBM]) diet or diets containing unheated SBM or SBM roasted at 165 degrees C for 75, 150, 180, or 210 min in a 6 x 6 Latin square experiment. Concentrations of indicators of heat exposure (ADIN, ADF, NDF) in SBM increased with increasing roasting time. Duodenal flows of total N, non-bacterial N, and SBM N increased (P < .05) linearly with increased roasting time. Small intestinal (SI) digestibility (percentage entering SI) of total N and SBM N was influenced quadratically (P < .05) by roasting time; SI digestibilities were modestly increased by heating SBM to 150 min, then declined dramatically when SBM was heated for 180 and 210 min. These responses resulted in a quadratic (P < .05) increase in quantity of total and SBM N disappearing in the SI; quantities increased as SBM was heated to 150 min and remained unchanged when SBM was heated for 180 and 210 min. These responses resulted in a quadratic (P < .05) increase in quantity of total and SBM N disappearing in the SI; quantities increased as SBM was heated to 150 min and remained unchanged when SBM was heated for 180 and 210 min. Duodenal flows and SI digestibilities of total and non-bacterial amino acids (AA) and of most individual AA followed patterns similar to those observed for N. Maximum quantities of total and individual AA disappeared from the SI when wethers were fed SBM roasted at 165 degrees C for 150 min. Evaluation of the effects of heat treatment on the nutritive value of a protein source for ruminants should include considerations for both ruminal protein escape and SI availability of escaped protein and for the nature of the AA supply absorbed from the SI.

Amino Acids↗

Effect of prenatal androgenization on performance, lactation, carcass, and sensory traits of heifers in a single-calf heifer system.

Twenty-four prenatally androgenized (PA) heifers, born to testosterone propionate-implanted cows, and 27 control (C) heifers, born to nonimplanted cows, were bred, calved, and placed in drylot pens, with calf at side, to assess the effects of PA on feedlot growth and lactational performance of heifers used in a single-calf heifer (SCH) system. Lactating heifers were fed an 85% concentrate diet, calves weaned at 117 d postpartum, and heifers slaughtered at a constant compositional end point of 1.1 cm of s.c. fat. Preweaning, PA heifers gained 22.5% faster (P < .01) and were 17.3% more efficient (P < .01) than controls. Prenatal androgenization of the heifer, however, did not affect calf growth performance. All calves gained more than 1.4 kg/d and had an average weaning weight of 159 kg at 117 d of age. Combining heifer-calf weights, PA and C pairs gained 2.88 and 2.53 kg/d, respectively. Correcting for DMI of calves, PA improved (P < .01) efficiency of beef production 10.8%. Four percent fat-corrected milk yield of PA and C heifers averaged 7.96 and 7.60 kg/d, respectively, and the percentage of milk protein and fat did not differ. Accounting for average BW, milk production, and DMI, PA and C lactating heifers should have gained 1.27 kg/d; however, gains were 137 and 127% of those predicted, respectively. Overall, pre- and postweaning, PA heifers required 10 fewer (P < .04) days on feed, gained 23% faster (P < .01), and were 16% more efficient (P < .02) than C heifers. At slaughter, PA and C heifers averaged 853 and 865 d of age, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

Prenatal androgenization of lambs: I. Alterations of growth, carcass characteristics, and metabolites in blood.

Eight ewe lambs (TE) born to ewes implanted with testosterone propionate between d 40 and 70 of gestation and 10 ewe lambs (CE) and 10 wether lambs (CW) from non-implanted ewes were used to measure the effects of prenatal androgenization on growth and metabolism. Starting BW (approximately 60 d of age) were not different among treatment groups. Finishing BW of TE and CW tended to be greater (P < .10) than those of CE; ADG of TE was 13% greater (P < .05) than ADG of CE. The DMI tended to be greater (P < .10) for TE than for CE but feed efficiency did not differ among treatments. Carcass weights were greater (P < .05) for CW than for CE. Subcutaneous fat, internal fat, and longissimus muscle area were not affected by sex or prenatal androgenization. Increases in glucose concentration in plasma from prefeeding to postfeeding were greater (P < .05) for CE than for TE or CW. The concentration of NEFA in plasma decreased (P < .05) from growing to finishing stages for TE and CW but remained constant for CE. Plasma glycerol concentration decreased more (P < .05) after feeding for TE and CW than for CE. Average concentrations of insulin (P < .01) and the increase of insulin after feeding (P < .05) were greater for TE and CW than for CE. The average concentration of urea N in plasma tended (P < .08) to be greater for CW than for TE or CE.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

Prenatal androgenization of lambs: II. Metabolism in adipose tissue and liver.

In vitro measurements of metabolism were made in subcutaneous and perirenal adipose tissue (AT) and liver from prenatally androgenized ewe lambs (TE), control ewe lambs (CE), and control wether lambs (CW). In adipose tissue slices, release of glycerol or fatty acids into the medium was not different among treatments, but glycerol release was greater (P < .01) from subcutaneous AT than from perirenal AT. Basal fatty acid release and the free fatty acid pool were greater (P < .05) for perirenal AT than for subcutaneous AT; fatty acid release and the fatty acid response (increased NEFA in media and tissue) were increased more by lipolytic stimuli in subcutaneous AT than in perirenal AT. Adipose tissue from CW had the greatest (P < .05) fatty acid response under conditions of near-maximal stimulation; rates from TE were intermediate to those from CW and CE. Incorporation of glucose into fatty acids and glycerol in subcutaneous AT was lowest (P < .05) for TE. Oxidation of glucose and acetate to CO2 and incorporation of acetate into fatty acids or glycerol in subcutaneous AT, glucose and acetate metabolism in perirenal AT, and cellularity measurements for both AT did not differ among treatments. In liver slices, oxidation of [1-14C]propionate to CO2 was greater (P < .05) for CE than for TE or CW, and gluconeogenic capacity from [1-14C]propionate tended to be greater (P < .10) for CE than for TE. Glucose and CO2 production from [2-14C]propionate, [U-14C]alanine, or [U-14C]glycerol and total and peroxisomal first cycle of beta-oxidation of [1-14C]palmitate were not altered by prenatal androgenization or sex. There were no effects (P > .1) of prenatal exposure to testosterone on mitochondrial protein content of liver, rates of mitochondrial state 3 or state 4 respiration, the ratio of ADP:oxygen in the presence of respiratory substrates, or hepatic contents of lipid, triglyceride, or glycogen. Protein content of liver was greater (P < .05) for CW than for CE; TE were intermediate. Collectively, there were minimal modifications of in vitro metabolism in AT or liver attributable to prenatal androgenization or sex that would directly influence ADG and carcass composition.

Acetates↗

Performance and nutrient metabolism by nursing calves supplemented with limited or unlimited corn or soyhulls.

Five-month-old Angus x Hereford reciprocal crossbred nursing steer calves on fescue pasture were used to determine the effects of 1) limiting supplemental feed intake and 2) soyhulls and corn as sources of supplemental (creep) feed in an 84-d study. The treatments were 1) control (no creep feed), 2) limited intake of corn (1 kg/d), 3) unlimited intake of corn, 4) limited intake of soyhulls (1 kg/d), and 5) unlimited intake of soyhulls. Before weaning, there were no differences in supplemental feed efficiency ([supplemented gain--control gain]/supplement intake) or calf gain between corn or soyhulls. Calf gain before weaning increased linearly (P < .05) as intake of creep feed increased, but supplemental feed efficiency did not differ between limited and unlimited supplements. Feedlot performance by the calves was not affected by any of the previous treatments. Calves that consumed an unlimited level of creep feed had greater (P < .05) quality grades than the control group that did not consume creep feed, but no other carcass traits were influenced by treatment. In a metabolism study, fescue DM intake decreased linearly (P < .001) with increasing levels of creep feed, whereas milk DM intake was not affected by level of creep feed. Digestible DM intake increased linearly (P < .001) with increasing intake of creep feed. No differences (P < .18) were observed in digestible DM intake as a result of creep feed source. Apparent total tract DM digestibility increased (P < .05) with increasing level of creep feed intake but did not differ between sources.(ABSTRACT TRUNCATED AT 250 WORDS)

Animal Feed↗

The value of creep feeding during the last 84, 56, or 28 days prior to weaning on growth performance of nursing calves grazing endophyte-infected tall fescue.

To evaluate limiting the number of days that calves are creep fed, 84 crossbred cows (frame score 4) nursing steer calves were randomly assigned to three replicates over 2 yr to receive one of four lengths of creep feeding (0, 28, 56, or 84 d) for cows and calves grazing endophyte-infected tall fescue. There were no differences in cow performance due to treatments. Calf daily gain increased (P < .001) as the length of time exposed to creep increased. In yr 1, creep intake increased (P < .05) as the length to time exposed to creep increased. In yr 2, there was no difference in intake by period among 28-, 56-, and 84-d treatments. Supplemental feed efficiency was best for 56 and 84 d, and the 28-d treatment was extremely poor. During the feedlot phase, there were differences in performance between the 2 yr and there were no differences in carcass composition due to length of time receiving creep feed. Overall, creep feeding calves for 56 or 84 d improved performance, and the 56-d calves had the most efficient gain. Creep feeding calves for 28 d showed no advantage during the creep feeding period or in the feedlot. Four ruminally fistulated nursing steer calves were used in a 4 x 4 Latin square design to receive treatments of forage alone or .68 kg, 1.13 kg, or 2.27 kg/d of creep plus high-quality, freshly harvested forage available on an ad libitum basis. Forage OM intake and NDF digestibility tended (P = .11) to decrease as intake of creep feed increased. The pH decreased (P < .001), molar proportion of propionate tended to increase (P = .06), and acetate tended to decrease (P = .07) as the level of creep feed intake increased. Higher levels of creep feed tended to cause a decrease in ruminal fiber digestibility and forage intake. Creep feeding calves for 56 or 84 d improved gain; 56 d had the most efficient supplemental gain. Creep feeding calves for 28 d showed no advantage during the creep feeding period or in the feedlot.

Adipose Tissue↗

Effects of chemically treated, recycled newsprint on feed intake and nutrient digestibility by growing lambs.

In situ experiments and two lamb digestion trials were conducted to evaluate the effects of chemically treating recycled newsprint (NP). Treatment of NP with 2% HCl (percentage of NP DM) followed by autoclaving for 4 h was most efficacious and was used in an in vivo digestion trial with 25 ram and wether lambs in a randomized complete block design (RCB). On a DM basis treatment diets were: 1) control, 95% alfalfa hay (AH); 2) 75% AH, 20% HCl-treated NP (HCl-NP); 3) 55% AH, 40% HCl-NP; 4) 75% AH, 20% untreated NP; and 5) 55% AH, 40% untreated NP on a DM basis. Lambs fed Treatments 2 and 3 had ad libitum DMI (1,043 and 1,036 g/d, respectively) similar to the DMI of those fed the control treatment (1,024 g/d); however, lambs fed Treatments 4 and 5 had lower (P < .05) ad libitum DMI (578 and 426 g/d, respectively) than lambs fed Treatments 1, 2, and 3. Apparent DM digestibilities were lower (P < .05) by lambs consuming Treatments 2, 3, 4, and 5 (52.6, 44.5, 52.0, and 48.2%, respectively) than by those consuming Treatment 1 (60.7%). Apparent OM, CP, NDF, and ADF digestibilities followed trends similar to DM. In the second digestion trial, 15 ram and wether lambs were used in a RCB to compare Treatments 1, 2, and 3. For this second digestion study, NP was treated with 4% HCl (percentage of NP DM), autoclaved for 4 h, and diets were fed at 2% of BW. As in Trial 1, apparent nutrient digestibilities decreased (P < .05) with increasing amounts of HCl-NP. Our results suggest that HCl-NP can be fed to sheep at 40% of the diet without a decrease in DMI; however, apparent nutrient digestibility decreases with increasing levels of HCl-NP.

Animal Feed↗

Effects of cobalt on in vitro fiber digestion of forages and by-products containing fiber.

Cobalt glucoheptonate as a source of Co to enhance ruminal fiber digestion was evaluated in two in vitro digestibility experiments. In Experiment 1, Co supplementation (0, 5, and 10 ppm) of five substrates (leaf and stem fractions of alfalfa and orchardgrass hays and ground corn) was evaluated under two dietary conditions (ruminal fluid taken from steers fed alfalfa hay or a high concentrate diet) for 24 or 48 h of fermentation in a 3 x 5 x 2 x 2 factorial arrangement. In Experiment 2, four concentrations of Co (0, 10, 20, and 30 ppm) were added to five substrates (alfalfa hay, orchardgrass hay, corn cobs, recycled newsprint treated with HCl, and cellulose casings) and were incubated with ruminal fluid from steers fed alfalfa hay for 24 or 48 h of fermentation in a 4 x 5 x 2 factorial arrangement. No interactions among treatments were observed for digestibilities of DM, OM, or NDF in both experiments or for VFA concentrations in Experiment 1. Supplementation with Co did not increase digestibilities of DM, OM, or NDF in either experiment or concentrations of VFA in Experiment 1. In Experiment 1, in vitro digestibilities of DM, OM, and NDF were higher for inoculum from steers fed alfalfa versus concentrate. In Experiment 2, digestibilities of DM, OM, and NDF were highest for alfalfa hay and lowest for recycled newsprint treated with HCl. Cobalt concentrations that were above minimum requirements did not improve digestion of DM, OM, or fiber.

Animals↗

An integrated, dynamic model of feed hydration and digestion, and subsequent bacterial mass accumulation in the rumen.

Hydration of feeds and bacterial attachment to feed particles are thought to play major roles in rumen digestion of fibrous feedstuffs. The objective of the present study was to integrate these phenomena in a mechanistic model that could be used for data analysis. The proposed model was based on the conversion of biomass, where digestion end-products can be used for the synthesis of bacterial mass. Digestion of the potentially digestible fraction and subsequent accumulation of bacterial mass was based on a sequential, three-compartment model. These compartments represented substrate undergoing hydration, digestion, and bacterial mass accumulation. A fraction of the substrate was used for synthesis of bacterial mass. It was assumed that these bacteria associate either temporarily or permanently with the remaining substrate. Dacron bags containing either dry or fully-hydrated lucerne (Medicago sativa), maize (Zea mays) cobs, orchard grass (Dactylis glomerata), and wheat straw were incubated in the rumen of a steer that was infused continuously with (15NH4)2SO4. The 15N-enrichments of isolated particle-associated bacteria and residue remaining in the bags were used to estimate bacterial attachment. Substrate remaining and microbial mass accumulation were analysed simultaneously. Hydration did not appear to limit digestion. Fractional rate of digestion and appearance of attached bacterial mass was fastest for lucerne. For lucerne, 5% of the digestion end-products were used for synthesis of bacteria that associated with the substrate, whereas for maize cobs, orchard grass, and wheat straw this was 16, 14, and 19% respectively. Less than 2% of digestion end-products were used for synthesis of bacteria that permanently remained associated with the substrate. Permanent association can occur only with the indigestible fraction, and probably represents bacterial debris. Lysis and/or detachment of bacterial cells was highest for lucerne, and was indicative of the rapid dynamics of lucerne digestion.

Animal Feed↗

Digestion kinetics of alfalfa and wheat straw assuming heterogeneity of the potentially digestible fraction.

Although it is recognized that the potentially digestible fraction of substrates in the rumen is heterogeneous in composition, most deterministic models that describe ruminal in situ digestion assume existence of only one or two homogeneous fractions of potentially digestible material. Alternatively, a stochastic model based on heterogeneity of substrate has been developed, although its validity has not been determined. To evaluate such a model, alfalfa hay and wheat straw were physically fractionated by dry sieving. Dacron bags containing these fractions were incubated in the rumen of a steer and the residue that remained was analyzed sequentially for DM, NDF, ADF, ADL, and acid detergent insoluble ash. Digestion kinetics of chemical fractions were determined by simultaneous analysis of the digestion profiles of DM, NDF, ADF, ADL, and acid detergent insoluble ash. The weighted mean and variance of the fractional digestion rate constant were .083 h-1 and 4.7 x 10(-4) h-2 for alfalfa and .021 h-1 and 2.4 x 10(-5) h-2 for wheat straw, respectively. In contrast, fitting DM data to a stochastic model assuming heterogeneous rates, the mean and variance of the fractional digestion rate were .078 h-1 and 8.3 x 10(-11) h-2 for alfalfa and .018 h-1 and 5.3 x 10(-12) h-2 for wheat straw, respectively. Because the estimates of the variance approached zero for the stochastic model, it is implied that this model reduced to a model involving a single fractional digestion rate. It is concluded that parameter estimates obtained from a stochastic model do not necessarily have a biological meaning.

Animal Feed↗

Technical note: mineral deposits on Dacron bags during ruminal incubation.

Extensive DM contamination was found on Dacron bags that were incubated for prolonged periods of time in the rumen of steers fed alfalfa hay. The ash content of the contaminant was high, and most of it was acid-soluble X-ray analysis indicated the presence of hydroxylapatite and synthetic calcium magnesium phosphate or whitlockite. The contaminant appeared as a smooth coating on the Dacron fiber, suggesting that contamination was a gradual process rather than the result of entrapment of dislodged crystals from plant material. Contamination seemed to occur exponentially within the range of observations (0 to 42 d). Contamination also occurred in steers fed orchardgrass, although to a lesser extent than in steers fed alfalfa hay. The DM contamination was less than .04 g per bag (average bag weight was 1.2 g) during the first 10 d of incubation. However, correction for contamination might be required for studies involving longterm incubation or mineral digestion.

Animal Feed↗

Utilization of alkaline hydrogen peroxide-treated wheat straw in cattle growing and finishing diets.

Two experiments were conducted to evaluate alkaline hydrogen peroxide-treated wheat straw (AHPWS) in cattle growing (Exp. 1) and finishing (Exp. 2) diets. In Exp. 1, 162 crossbred steers (257 kg) were fed 66% roughage diets in an 84-d growth trial to compare AHPWS to corn silage (CS) and to evaluate different supplemental CP sources and levels. A completely randomized design with a 3 x 3 factorial arrangement of treatments was used. Factors were roughage source (CS, a 1:1 mixture of CS:AHPWS [MIX] and AHPWS) and CP treatment (13 and 11% CP with supplemental CP provided by soybean meal [13-SBM] and [11-SBM] and 11% CP with a combination of urea, corn gluten meal, and fish meal [UGF]). Lasalocid was fed at the rate of 200 mg per steer daily. Steers fed AHPWS had decreased (P less than .01) DMI compared with steers fed MIX and CS. This may be due to increased dietary Na from residual Na in AHPWS. With each incremental increase in AHPWS, ADG and gain/feed decreased (P less than .01). Dry matter intakes (kg/d), ADG (kg), and gain/feed for CS, MIX, and AHPWS were 8.0, 1.56, and .19; 8.2, 1.33, and .16; and 7.5, 1.08, and .14, respectively. Decreased performance by steers fed AHPWS may be due, in part, to a negative interaction between the lasalocid and dietary minerals. There were no differences in performance due to CP supplementation. In Exp. 2, AHPWS was compared to alfalfa hay (AH) and CS at 10 and 20% of dietary DM (2 x 3 factorial) in a 127-d finishing trial with 108 crossbred steers (341 kg).(ABSTRACT TRUNCATED AT 250 WORDS)

Animal Feed↗

Effects of supplemental protein source and level of urea on intestinal amino acid supply and feedlot performance of lambs fed diets based on alkaline hydrogen peroxide-treated wheat straw.

Two experiments were conducted to determine the effects of supplemental CP source and level of urea on intestinal amino acid (AA) supply and feedlot performance of lambs fed diets based on alkaline hydrogen peroxide-treated wheat straw (AHPWS). In Exp. 1, five cannulated (ruminal, duodenal, and ileal) crossbred wethers (61 kg) were used in a 5 x 5 Latin square design. Treatments consisted of different sources of CP and included soybean meal (SBM), a combination of urea, distillers dried grains (DDG), and fish meal, each provided an equal portion of supplemental CP (UDF), and three levels of urea (17, 33, and 50% of supplemental CP) fed in combination with DDG (U17, U33, and U50). Organic matter and N digestibilities decreased (P less than .05) when lambs were fed U17 compared with those fed SBM. There were no differences (P greater than .05) in bacterial N or AA flows to the duodenum due to CP source despite large differences in ruminal NH3 N concentrations and lower ruminal OM digestion when lambs were fed U17. Duodenal nonbacterial N and AA flows were highest (P less than .05) in lambs fed U17 and UDF and lowest when lambs were fed U50 and SBM. Lysine concentration in duodenal digesta decreased with incremental increases in DDG. In Exp. 2, 30 individually penned ram lambs (33 kg) were allotted to five CP treatments in a randomized complete block design. Treatments were similar to those of Exp. 1, with the exception that U17 was replaced by a 14% CP diet with SBM as the supplemental CP source; all other diets were formulated to contain 12% CP. Lambs fed U50 had decreased (P less than .08) ADG and gain/feed compared with all other treatments, and lambs fed UDF had greater (P less than .05) ADG and gain/feed than lambs fed U33. It was concluded that 17% of the supplemental CP from urea seems adequate to maximize bacterial protein synthesis and that no more than 33% of the supplemental CP should be provided by urea in diets based on AHPWS. Feeding a combination of ruminally resistant protein sources with complementary AA profiles of lysine and methionine (UDF) may enhance quality of protein entering the duodenum and feedlot performance.

Amino Acids↗