Biomedical subjects

L L Berger

Publications and source records attributed to L L Berger.

At least 73 records · Page 4Linked to original sources

PubMed1988

Effects of alkaline hydrogen peroxide treatment on in vitro degradation of cellulosic substrates by mixed ruminal microorganisms and Bacteroides succinogenes S85.

The effects of sodium hydroxide (NaOH) and alkaline hydrogen peroxide (AHP) treatments on wheat straw (WS) and various cellulosic substrates were determined by measuring susceptibility to degradation by mixed ruminal organisms or Bacteroides succinogenes S85. In vitro incubations were used to measure differences in fermentation resulting from each successive step in the AHP treatment process. In vitro incubations through 48 or 108 h were conducted to measure these differences. The AHP treatment of WS increased (P less than 0.05) dry matter, neutral detergent fiber, and acid detergent fiber degradation over control WS when these substrates were incubated with mixed ruminal microorganisms or B. succinogenes S85. Fermentations containing AHP-treated WS had greater (P less than 0.05) microbial purine (RNA) and volatile fatty acid concentrations by 12 h compared with those containing untreated or NaOH-treated WS. Xylose in AHP-treated WS was utilized more extensively (P less than 0.05) by 12 h compared with the xylose of untreated or NaOH-treated WS. Treatment with AHP removed 23% of the alkali-labile phenolic compounds from WS. When substrates with high levels of crystalline cellulose (raw cotton fiber, Solka floc, and Sigmacell-50) were treated with NaOH or AHP and incubated for 108 h with B. succinogenes S85, extent of acid detergent fiber degradation of cotton fiber and Sigmacell-50 was similar to that of their respective controls. Sodium hydroxide and AHP treatments were effective in increasing acid detergent fiber degradation of the Solka floc which contained, on average, 3.3 and 4.8 percentage units more acid detergent lignin and hemicellulose, respectively, than cotton fiber and Sigmacell-50.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

PubMed1988

Effects of alkaline hydrogen peroxide treatment of cotton and wheat straw on cellulose crystallinity and on composition and site and extent of disappearance of wheat straw cell wall phenolics and monosaccharides by sheep.

Effects of alkaline hydrogen peroxide (AHP) treatment on cellulose crystallinity and cell wall phenolic monomer and monosaccharide composition were measured using cotton and wheat straw (WS). Two WS treatments were used in this study, Type I WS, for which pH is not regulated during AHP treatment, and Type II WS, for which pH is regulated at 11.5 +/- .2 during AHP treatment. Wheat straw had a lower degree of cellulose crystallinity than cotton, but no differences occurred between treated and untreated substrates. Alkali-labile and nitrobenzene-extractable phenolic monomer concentrations were generally lower for Type I and Type II WS compared with untreated WS. Concentrations of glucose were higher and xylose and arabinose lower in Types I and II WS than in untreated WS. Disappearance of alkali-labile phenolic monomers and cell wall monosaccharides by wethers fed diets containing Type I (Exp. 1) or Type II (Exp. 2) AHP-treated WS were determined. Apparent digestibility of glucose and xylose before the duodenum, and of glucose, xylose and arabinose in the total tract, was greatest (P less than .05) when sheep were fed AHP-treated WS diets in both experiments. In Exp. 2, disappearance of alkali-labile phenolic monomers was greatest (P less than .05) before the duodenum and in the total tract when sheep were fed AHP-treated WS diets. Treatment of WS with AHP modified cell wall composition and increased cell wall monosaccharide digestion by sheep.

Animal Feed↗

PubMed1988

Low-quality roughages in high-concentrate pelleted diets for sheep: digestion and metabolism of nitrogen and energy as affected by dietary fiber concentration.

Two trials were conducted to evaluate effects of, and interactions between, level and source of fiber in the diet on ruminal environment, microbial protein synthesis, nutrient digestion and flow of digesta through the gastrointestinal tract of multiple-fistulated sheep (trial 1; 4 X 4 Latin square design) and on ruminal, digestive and metabolic characteristics of early-weaned lambs (trial 2; randomized complete block design; 3 periods). All diets tested were pelleted and were formulated to contain either 39% or 25% neutral detergent fiber (NDF), with corncobs or cottonseed hulls (CSH) as the major NDF (roughage) sources. In trial 1, dry-matter (DM) and organic-matter (OM) digestibilities were not different (P greater than .05) among treatments. Digestibility of NDF was higher (P less than .05) with high-fiber. Bacterial N synthesis (g N/kg OM truly digested) was not different (P greater than .05) among treatments. Molar proportion acetate was higher (P less than .05) and molar proportion propionate lower (P less than .05) when sheep were fed high-fiber diets. In trial 2, apparent DM digestibility was higher (P less than .05) for lambs fed diets containing corncobs. Energy digestibility was higher (P less than .05) at the low-fiber level and for lambs fed diets containing corncobs. Apparent NDF digestibility by lambs was higher (P less than .05) at the high-fiber level and for lambs fed diets containing corncobs. Nitrogen retained (percentage of N intake) was higher (P less than .05) for lambs fed diets containing CSH. Ruminal pH and molar proportion acetate were higher (P less than .05) and molar proportion propionate lower (P less than .05) for lambs fed high-fiber diets. Although responses to corncob vs CSH inclusion in high-energy pelleted diets differ, both roughages are effective as fiber sources in sheep diets.

Animal Feed↗

PubMed1988

Low-quality roughages in high-concentrate pelleted diets for sheep: digestion and metabolism characteristics as affected by corncob and(or) rice hull additions.

Two trials were conducted to determine effects of feeding corncobs, rice hulls or a combination of both on nutrient digestion and flow of digesta through the gastrointestinal tract of multiple-cannulated sheep (55-kg Rambouillet X Dorset rams; trial 1; 4 X 4 Latin square) as well as ruminal, digestive and metabolic characteristics of early-weaned lambs (24.1-kg crossbred rams; trial 2; randomized complete block design; 3 periods) fed pelleted 75% concentrate-25% roughage diets. In trial 1, apparent total tract dry matter (DM), organic matter (OM) and neutral detergent fiber (NDF) digestibilities decreased and N digestibility increased as percentage of dietary corncobs decreased and percentage of rice hulls increased. Site of NDF digestion appeared to shift from the upper to the lower digestive tract as percentage of corncobs decreased and percentage of rice hulls increased. In trial 2, apparent DM digestibility decreased as corncobs were replaced with rice hulls in the diet. Neutral detergent fiber digestibility was not affected by diet except in larger lambs at higher intakes, with NDF digestibility generally decreasing as percentage of dietary rice hulls increased. Nitrogen digestibility was not affected by replacing corncobs with rice hulls in the diet. Ruminal pH decreased as rice hulls replaced corncobs in the diet. These data are interpreted to indicate that rice hulls can be effectively substituted for corncobs in sheep diets when added at levels up to 25% of the total diet.

Animal Feed↗

PubMed1988

Effects of dietary energy level and protein source on site of digestion and duodenal nitrogen and amino acid flows in steers.

Six steers (468 kg) with ruminal and duodenal cannulas were fed diets formulated for two levels of energy containing three crude protein (CP) sources in a 6 X 6 Latin square with a 2 X 3 factorial arrangement of treatments. Energy levels were 2.17 and 2.71 Mcal metabolizable energy (ME)/kg dry matter (DM) provided by hay-corn (H) and corn silage-corn (CS) diets, respectively. Soybean mean (SBM), corn gluten meal-urea (CGM) and urea (U) provided 33% of dietary CP in 12% CP diets. Apparent organic matter (OM) digested in the stomach was not affected (P greater than .05) by energy level or CP source, but OM truly digested in the stomach was greater (P less than .05) when steers were fed the CS compared with the H diet. Duodenal flow of non-NH3 N was greater (P less than .05) when steers were fed CS compared with H and when fed SBM or CGM compared with U. Efficiency of bacterial protein synthesis and duodenal bacterial N flow were increased (P less than .05) when steers were fed CS, but non-NH3, nonbacterial N flow to the duodenum was increased (P less than .05) when steers were fed H. When steers were fed CS rather than H, flows (g/d) of bacterial amino acids were greater (P less than .05), but flows of nonbacterial amino acids tended (P less than .08) to be less. Total amino acid flows were not affected (P greater than .05) by energy level. Duodenal flows of total amino acids tended (P less than .06) to be greater when steers were fed CGM compared with SBM or U, due mainly to an increased (P less than .05) flow of nonessential amino acids.

Amino Acids↗

PubMed1988

Effect of prenatal androgenization on growth performance and carcass characteristics of steers and heifers.

Two experiments were conducted in which pregnant crossbred cows were randomly assigned to a control group or implanted with testosterone propionate/silastic implants between d 110 and 140 of gestation in Exp. 1 and between d 80 and 110 of gestation in Exp. 2. Androgenized heifers (TH) and treated steers (TS), born to implanted dams, possessed similar birth weights compared with control heifers (CH) and steers (CS), respectively, in both experiments. In Exp. 1, yield grades were lower (P less than .05) for TH than for CH. In Exp. 2, TH possessed 9.4% greater (P less than .07) 205-d adjusted weaning weights and 9.8% heavier (P less than .05) adjusted yearling weights than CH. In Exp. 2, daily gain was 19.5% faster (P less than .05) and feed intake was 13.6% greater (P less than .05) for TH than for CH. In Exp. 2, TH possessed less (P less than .05) s.c. fat, greater (P less than .10) carcass weight gains and lower (P less than .05) yield grades than CH. Liver weight per unit of carcass weight was greater (P less than .07) for TH than for CH in Exp. 2. Androgenized heifers had lower (P less than .07) lipid content in the 9-10-11th rib section than did CH. Calving intervals were similar for implanted and nonimplanted cows in both experiments. Results from these two trials suggest that efficiency of heifers for producing carcass beef can be improved by prenatal testosterone exposure.

Animals↗

PubMed1988

Estimation of lysine and methionine requirements of growing steers fed corn silage-based or corn-based diets.

Methionine and lysine requirements of growing steers were studied. Steers (Experiment 1, 313 kg) fed 6.4 kg of an 80% corn silage diet (1.38% N) had duodenal flows of methionine, cysteine, and lysine of 9.7, 10.7, and 40.2 g/d. Linear increases in plasma methionine and plasma lysine in response to abomasal infusions of DL-methionine and L-lysine indicated that requirements for methionine and lysine were met by basal flows or that neither lysine nor methionine was the first or sole limiting amino acid. Steers (Experiment 2, 383 kg) fed 7.5 kg of a 75% corn diet (1.55% N) had methionine, cysteine, and lysine flows at the duodenum of 13.8, 16.1, and 52.9 g/d. Abomasal infusions of DL-methionine resulted in linear increases in plasma methionine, but abomasal infusions of L-lysine resulted in two-phase broken line plasma amino acid response curves. For steers infused with lysine alone, the breakpoint occurred at 4 g/d infused lysine, whereas steers receiving 9 g/d DL-methionine had a breakpoint at 8 g/d infused lysine. The total absorbable lysine requirement was estimated to be 44 to 48 g/d, depending upon methionine status. Rumen-protected amino acids fed to steers on both dietary regimens were effective in supplying methionine and lysine postruminally.

Animal Feed↗

PubMed1988

Proteolysis of alcohol-treated soybean meal proteins by Bacteroides ruminicola, Bacteroides amylophilus, pepsin, trypsin, and in the rumen of steers.

Sodium dodecyl sulfate-gel electrophoresis and cation exchange chromatography were used to examine degradation of treated and untreated soybean meal protein fractions by Bacteroides amylophilus H18(1), Bacteroides ruminicola B(1)4, pepsin, trypsin, and intraruminally. Soybean meal treatments consisted of 30% vol/vol isopropanol, 40% propanol, or 50% ethanol at 22 degrees C or 70% ethanol at 80 degrees C. Water-soluble protein fractions were applied to a hydroxylapatite column and eluted with a discontinuous phosphate gradient of .03 to .27 and then .27 to 1.0 M. The four protein fractions with the highest absorbance at 276 nm were dialyzed against distilled water prior to being subjected to enzymatic hydrolysis. Soybean meal treated with 40% propanol had the greatest reduction in absorbance of all effluents at 275 nm, followed by soybean meal treated with 50% ethanol or 30% isopropanol. Comparison of electrophoretic patterns over time showed that B. amylophilus H18(1), degraded protein subunits more rapidly than B. ruminicola B(1)4. Protein subunits with the highest molecular weights were the most rapidly degraded by B. amylophilus H18(1), B. ruminicola B(1)4, pepsin, and trypsin. Hydroxylapatite chromatography of omasal fluid from steers supplemented with untreated soybean meal or soybean meal treated with 70% ethanol at 80 degrees C indicated that no detectable soluble glycinin or conglycinin escaped ruminal degradation.

Animals↗

PubMed1987

Effects of ammoniation of tall fescue on phenolic composition, feed intake, site and extent of nutrient digestion and ruminal dilution rates of steers.

Tall fescue hay (H) supplemented with corn and urea (HU) or corn gluten meal (HCGM) and ammoniated tall fescue hay supplemented with corn (AH) or corn gluten meal (AHCGM) were fed to steers in two 4 X 4 Latin-square trials. Diets were fed to four Angus-Hereford steers (550 kg) at equal intakes in trial 1 and to four Hereford steers (350 kg) at ad libitum intakes in trial 2. Ammoniation reduced cell wall concentrations of p-coumaric acid and ferulic acid by 48 and 67%, respectively. Concentrations of other phenolics were also reduced. Apparent total tract digestibilities of vanillin, p-coumaric acid and ferulic acid were lower (P less than .05, .001 and .01, respectively) when nontreated hay was fed in trial 1, but were not different between hay types in trial 2. In trial 1, greater negative intestinal digestibilities of p-coumaric acid (P less than .001) and vanillin (P less than .05) occurred for steers fed HU and HCGM vs AH and AHCGM diets. Digestibilities of neutral detergent fiber (NDF) and acid detergent fiber (ADF) were greater (P less than .001) for steers fed ammoniated hay diets in both trials and greater (P less than .05) for HCGM vs HU in trial 1. More than 96% of the NDF and ADF digested by steers in trial 1 was digested in the stomach. Intakes of digestible NDF and ADF, but not indigestible NDF or ADF, were higher (P less than .001) for steers fed AH and AHCGM in trial 2. In situ dry matter disappearance rate of ammoniated hay was greater (P less than .05) than that of nontreated hay, but rate of cotton thread disappearance from bags suspended in the rumen of steers fed the various diets was similar among treatments. In both trials, feeding ammoniated hay resulted in higher (P less than .05) ruminal concentrations of acetate and higher (P less than 0.05) acetate:propionate ratios. Ruminal liquid dilution rates were lower (P less than .05) for steers fed AH and AHCGM in trial 1, but were not different in trial 2. Ruminal dry matter concentration and solids dilution rate were not affected by diet in either trial. The results are interpreted to indicate that increased intake of ammoniated hay is a result of increased rate and extent of fiber digestion.

Ammonia↗

PubMed1987

Effects of heat and alcohol treatments of soybean meal on nitrogen utilization by sheep.

Soybean meal (SBM) was treated with aqueous solutions of ethanol or propanol at room temperature or at 80 C to study treatment effects on SBM-N solubility and utilization by sheep. Soybean meal was soaked in an excess of 70% (v/v) ethanol at 80 C (ET-80), 70% ethanol at 23 C (ET-23) or 70% propanol at 80 C (PR-80). Nontreated SBM and nontreated SBM heated at 80 C without alcohol treatment (NT-80) served as controls. Nitrogen solubility in McDougall's buffer was lowest (P less than .05) for PR-80 and ET-80 (2.2 and 4.7% of total N, respectively), intermediate (P less than .05) for ET-23 (9.0%), greater (P less than .05) for nontreated SBM (36.2%) and highest for NT-80 (40.2%). In an situ study using three ruminally cannulated cows and two bags per treatment per animal per removal time, more (P less than .05) N remained in in situ bags after 3, 6, 9 and 12 h incubation for ET-23, ET-80 and PR-80 than for nontreated SBM and NT-80. A lamb metabolism trial, using 15 lambs in each of two periods, compared nontreated SBM, ET-23, ET-80, PR-80 and urea as N supplements. Nitrogen retention was higher (P less than .02) for lambs fed SBM treatments compared with urea. When the same N supplements were fed to wethers in a 5 X 5 Latin square experiment and duodenal N flow was measured, non-ammonia non-bacterial N flow was higher (P less than .07) for wethers fed SBM treatments than for wethers fed urea.(ABSTRACT TRUNCATED AT 250 WORDS)

1-Propanol↗

PubMed1987

Evaluation of corn fiber, cottonseed hulls, oat hulls and soybean hulls as roughage sources for ruminants.

An in situ trial (randomized complete block design) using cows, and a site and extent of digestion trial (Latin square design) using sheep were conducted to study the potential of corn fiber (CF), cottonseed hulls (CSH), oat hulls (OH) and soybean hulls (SH) as roughage sources for ruminants. Two feedlot trials with steers and one with lambs (completely randomized design with factorial arrangements of treatments) were conducted to study the potential of CF and SH as energy supplements relative to corn. In situ rate of ruminal dry matter (DM) disappearance (3 to 36 h) and extent of DM disappearance (36 h) indicated that CF and SH were more fermentable in the rumen compared with OH or CSH, with SH being the most fermentable. Total tract digestibilities of DM, organic matter (OM), neutral detergent fiber (NDF) and acid detergent fiber (ADF) were above 70% for sheep fed CF and SH diets, and were 50% or less for sheep fed OH and CSH diets. A ranking of by-products in terms of nutritive value followed the trend: CF greater than SH greater than OH greater than CSH. Lamb feedlot trial data showed that CF was of similar nutritive value to corn and of higher nutritive value than SH at the 50% level of supplementation. Corn-fed lambs responded better than CF- or SH-fed lambs at the 70% level of supplementation. Data from steer feedlot trials showed that CF was of similar quality to corn and of higher quality than SH. Dramatic differences exist in by-product feed utilization by ruminants. All by-products tested appeared to have some usefulness as dietary components.

Animal Feed↗

PubMed1987

Effects of dietary energy level and supplemental protein source on performance of growing steers and nutrient digestibility and nitrogen balance in lambs.

Four experiments were conducted to evaluate three crude protein (CP) sources (urea, U; soybean meal, SBM; corn gluten meal, CGM) in diets based on corn silage (high energy) or grass hay (low energy). In Exp. 1 and 2, growing steers were fed all combinations of energy and protein source at 10.5 or 12% CP. Steers fed high energy diets or 12% CP had improved (P less than .05) daily gains and feed:gain over 84 d. Protein source had no effect (P greater than .05) on performance except that steers fed U consumed more (P less than .05) feed than those fed CGM. Steers were fed experimental diets to a common weight and switched to an 85% concentrate diet for finishing. During finishing, steers fed low energy diets in the growing period consumed more (P less than .05) feed and had increased (P less than .05) feed:gain compared with those fed high energy diets. Growing lambs were fed the same diets as steers. At 10.5% CP, lambs fed high energy diets had higher (P less than .05) digestibilities of dry matter (DM), organic matter (OM), nitrogen (N) and fiber components, and retained more (P less than .05) N. For lambs on 12% CP, high energy diets had higher (P less than .05) DM and OM digestibilities and lower (P less than .05) N digestibilities. At 12% CP, energy level had no effect (P greater than .05) on N retained. Protein source had no effect (P greater than .05) on N retention. There appeared to be no advantage in supplementing with ruminally undegradable proteins, i.e. CGM, in these experiments.

Animals↗

PubMed1987

Growth and carcass characteristics of cull cows after different times-on-feed.

Cull cows (N = 48) of similar age from Angus and Hereford breed types were assigned to one of four feeding periods (0, 28, 56 or 84 d) and subdivided into two groups, one implanted with Synovex-H and a non-implanted control. Cows were fed a high energy diet and slaughtered at the end of each feeding period. Average daily gain and feed efficiency were evaluated on each animal during the trial. The following carcass traits were evaluated: USDA yield and quality grade characteristics, boning yield, muscle weights, bone weights, pH and Warner-Bratzler shear force of the longissimus muscle. The left side of each carcass was boned and proximate analysis was determined. No significant implant effects were encountered for any of the traits evaluated; therefore, data were pooled and evaluated by feeding period only. Carcass weight and boneless forequarter and hindquarter tissue weights increased significantly between each feeding period; percent kidney, pelvic and heart fat (KPH) and yield grade did not differ (P greater than .05) until d 56 and 84, respectively. Quality grade and marbling scores improved (P less than .05) between 28 and 56 d of feeding. Biceps femoris weight changed during the 84-d experiment from 4.1 kg on d 0 to 5.3 kg on d 84 (P less than .05). Proximate analysis of the boneless tissue indicated an increase (P less than .05) in extractable fat of approximately 6% for each feeding period (6.3% fat on d 0 to 25.4% on d 84).(ABSTRACT TRUNCATED AT 250 WORDS)

Animal Nutritional Physiological Phenomena↗

PubMed1987

Effect of prenatal androgenization on lamb performance, carcass composition and reproductive function.

Two experiments were conducted in which pregnant crossbred ewes were randomly assigned to a control group or implanted with testosterone propionate/silastic implants between d 40 and d 60 of gestation. Implants contained approximately 2 g testosterone propionate and provided a constant delivery of 8.7 mg/d. Androgenized ewes (TE), born to implanted dams, had lower adjusted birth weights (P less than .05) than control ewes (CE) in both experiments. Treated rams (TR) also displayed lower birth weights than control rams (CR) in both experiments, but the difference was significant only in Exp. 2. Average daily gain was 16.4% more (P less than .05) for TE than for CE in Exp. 1. In Exp. 2, TE gained 9.6% faster (P less than .05) and had 14.6% (P less than .05) more weight per unit of feed consumed than CE. In both experiments, average daily gain and gain-to-feed ratio were similar for TR and CR. In Exp. 2, prenatal androgenization of ewes reduced (P less than .05) 12th rib fat thickness and decreased (P less than .05) percent kidney and pelvic fat. Yield grades of TE were lower than CE in both Exp. 1 (P less than .10) and Exp. 2 (P less than .05). From additional measurements taken in Exp. 2, liver weight per unit of carcass weight was greater (P less than .05), anogenital distance was greater (P less than .01) and test tube penetration into the vestibule was less (P less than .01) for TE than CE. The TE did not exhibit regular estrous cycles, while CE displayed recurring estrous cycles.

Animals↗

PubMed1987

Effects of ethanol and heat treatments of soybean meal and infusion of sodium chloride into the rumen on ruminal degradation and escape of soluble and total soybean meal protein in steers.

Soybean meal (SBM) treated with 70% ethanol at 80 C (ET), nontreated SBM (NT) or a ureacasein-corn mix (UC) was fed to steers fitted with ruminal and duodenal cannulae to study ruminal N metabolism. Sodium chloride (NaCl) was ruminally infused at 0 or 500 g/d. Nitrogen supplements provided approximately 70% of total dietary N. Experimental design was a 6 X 6 Latin square with a 3 X 2 factorial arrangement of treatments. Total duodenal N flows and non-ammonia, non-bacterial-N (NANB-N) flows were higher (P less than .05) when steers were fed SBM treatments compared with UC, and higher (P less than .05) when steers were fed ET compared with NT. Percentage of SBM-N escaping ruminal degradation was greater (P less than .05) when steers were fed ET compared with NT, and greater (P less than .05) when NaCl was infused into the rumen. Duodenal flows of total, indispensible and dispensible amino acids were increased (P less than .05) when steers were fed SBM treatments compared with UC, and greater (P less than .05) when steers were fed ET compared with NT. No differences in soluble N flows at the omasum were observed due to treatment. Bacterial protein comprised the majority of the N leaving the rumen. Both ruminal NaCl infusion and ethanol and heat treatment of SBM increased ruminal SBM-N escape.

Animals↗

PubMed1987

Effects of ethanol, heat, and lipid treatment of soybean meal on nitrogen utilization by ruminants.

Ruminant nitrogen utilization of soybean meal treated with 1) 70% ethanol at 23 or 78 degrees C, 2) 10% coconut oil or tallow, or 3) a combination of 70% ethanol at 78 degrees C and coconut oil or tallow was evaluated. Nitrogen solubility was lowest for soybean meal treated with ethanol at 78 degrees C, ethanol plus coconut oil and ethanol plus tallow. In situ nitrogen disappearance was lowest for soybean meal treated with ethanol at 78 degrees C, ethanol plus coconut oil, and ethanol plus tallow. Rates of nitrogen disappearance between 3 and 12 h were lowest for soybean meal treated with ethanol at 78 degrees C, ethanol plus coconut oil, and ethanol plus tallow. Nitrogen retained by lambs was greater for lambs fed soybean meal treated with ethanol at 78 degrees C than for those fed untreated soybean meal. Ruminal ammonia 4 h postfeeding was lowest for lambs fed soybean meal treated with ethanol at 78 degrees C, ethanol plus coconut oil, and coconut oil. These data indicate that the 78 degrees C ethanol treatment improved nitrogen utilization.

Animal Feed↗

PubMed1987

Effects of treating wheat straw with pH-regulated solutions of alkaline hydrogen peroxide on nutrient digestion by sheep.

An experiment using a 4 X 4 Latin square design was to determine effects of treating wheat straw with pH-regulated (pH = 11.5) solutions of hydrogen peroxide on site and extent of nutrient digestion in multiple-fistulated sheep. Regulating reaction pH at 11.5 prevented solubilization of some cell wall hemicelluloses, resulting in improved retention of DM. Diets fed to sheep contained 33 or 70% wheat straw either untreated or treated with alkaline hydrogen peroxide. Sheep fed diets of treated wheat straw digested more DM, NDF, ADF, and cellulose anterior to the duodenum and in the total tract than when fed diets of untreated wheat straw. Apparent CP digestion before the duodenum was highest when sheep were fed the treated 33% wheat straw diet and untreated 70% wheat straw diet. Treatments did not affect apparent nutrient digestibilities in the large intestine. Ruminal pH was lower when sheep were fed the alkaline hydrogen peroxide-treated or diets containing 33% wheat straw. Ruminal ammonia concentrations were highest when sheep were fed the untreated 70% wheat straw diet. Molar proportions of ruminal acetic and propionic acids were unaffected by diet. Alkaline hydrogen peroxide treatment substantially increased susceptibility of structural carbohydrates of wheat straw to microbial degradation in the gastrointestinal tract of sheep.

Animal Feed↗

PubMed1987

Ruminal nitrogen metabolism in steers as affected by feed intake and dietary urea concentration.

Four multiple-cannulated steers (340 kg) were used in a 4 X 4 Latin square design with a 2 X 2 factorial arrangement of treatments. Steers were fed a diet of 50% ground hay and 50% concentrate at two intakes (1.4 and 2.1% of BW), with urea and 15N-enriched ammonium sulfate infused continuously into the rumen at .4 or 1.2% of diet DM. Ratios of purines and diaminopimelic acid-N to N in fluid-associated and particulate-associated bacteria and in protozoa were similar among treatments but were lower for protozoa than for bacteria. Diaminopimelic acid-N:N was higher for fluid-associated vs. particulate-associated bacteria. Enrichment of 15N was similar between bacteria among treatments and was 30% lower for protozoa. Turnover rates of 15N in bacteria, NH3N, and non-NH3N pools were faster for steers infused with 1.2 than those infused with .4% urea, indicating less efficient usage of ammonia with higher urea. A method is described to estimate the proportion of duodenal nitrogen comprising bacterial and protozoal nitrogen.

Animal Feed↗