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L L Ivanov

Publications and source records attributed to L L Ivanov.

20 records · Page 2Linked to original sources
PubMedDate not supplied

[Amino acyl tRNA synthetase complexes in rabbit liver in experimental myocardial infarction].

The composition of high-molecular complexes of aminoacyl-tRNA-synthetases (ARSase) from the rabbit liver was studied on the first day after reproduction of the experimental myocardium infarction. The studies revealed an increase in the glutamyl-, leucyl-, lysyl- and a decrease in the glycyl- and seryl-tRNA-synthetase activities and redistribution of the last two enzymes from the composition of the complexes into a lower-molecular fraction of postribosomal supernatant fluid. Under the experimental myocardium infarction the complexes reveal a significant decrease in the content of phospholipids and variations in the electrophoretic mobility of protein fractions. An assumption is advanced that a disturbed protein biosynthesis in the liver resulted from the experimental myocardium infarction causes changes in the structural organization of the ARSase complexes.

Amino Acyl-tRNA Synthetases↗
PubMedDate not supplied

[Sulfhydryl groups of leucyl-tRNA synthetase from the cow mammary gland].

P-chloromercuribenzoate (PCMB) inhibition of the leucyl-t-RNA-synthetase (LeuRS) activity was studied both in the tRNA-aminoacylation and ATP-[32P]-pyrophosphate exchange reactions. It is established that the enzyme contained sulphydryl (SH) groups essential for catalytic activity. No protection of substrates from the PCMB action was observed in both reactions. It is shown that SH-groups essential for tRNA-aminoacylation were of no use for leucine activation. The PCMB modified enzyme was reactivated (80%) after the treatment with dithiothreitol. The quantity of cysteine residues in the native and denatured forms of LeuRS was determined.

Amino Acyl-tRNA Synthetases↗