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L L Kiselev

Publications and source records attributed to L L Kiselev.

At least 19 recordsLinked to original sources
PubMed1987

[A method for detecting protein-protein interactions. Detection of proteins with the molecular weight of approximately 37 kD binding with tryptophanyl-tRNA-synthetase].

A procedure for detecting protein-protein interactions is proposed. It is based on blotting of electrophoretically separated protein mixtures followed by detection of the proteins interacting with a given 125I-labelled protein used as a probe. Application of this approach to lysates of cultured mammalian cells enabled us to reveal a unique 37 kDa polypeptide interacting with 125I-labelled bovine tryptophanyl-tRNA synthetase (EC 6.1.1.2).

Amino Acyl-tRNA Synthetases↗
PubMed1986

[Localization of antigenic determinants using monoclonal antibodies and alpha-NH2-terminal labeling of polypeptide chains].

A method for localization of antigenic determinants in a polypeptide chain of unknown primary structure was proposed. A protein is modified at NH2-terminal and epsilon-NH2-groups of lysine residues with maleic anhydride and then is subjected to partial enzymatic cleavage. Newly formed NH2-terminal groups are tagged with radioiodinated Bolton--Hunter's reagent. The labeled fragments of the antigen are then demaleylated. Comparison of the two longest labeled fragments, only one of which still binds monoclonal antibody, makes it possible to define the location of the antigenic determinant along the polypeptide chain. The method was tested on the bovine tryptophanyl-tRNA synthetase using earlier prepared monoclonal antibodies against this enzyme.

Amino Acid Sequence↗
PubMed1986

[Insertion of the cos-site into DNA of phage M13 and its packing in proteins of phage lambda].

The cos-site of lambda phage from pHC79 cosmide is transferred to DNA from M13 mp18 phage. The recombinant DNA thus obtained (MC18) is efficiently packaged into lambda proteins in vitro. The BamHI-HindIII fragment of pGP588 (a pBR322 derivatives containing fragment of human DNA) is subcloned into MC18. Although this pGP588 fragment contains numerous Alu repeats, no essential rearrangements of the insert were revealed. The efficiency infection by recombinant DNA packaged with lambda proteins is about 1 X 10(5) pfu/microgram DNA, whereas in the similar conditions the efficiency of lambda EMBL3A was 1 X 10(6) pfu/microgram. It is assumed that the MC vectors might be suitable for cloning and sequencing large fragments either with cohesive or blunt ends. It opens also the way to construct genomic libraries in single-stranded phages.

Bacteriophage lambda↗
PubMed1986

[DNA of the hepatitis B virus in human generative cells].

In spermatozoa of certain patients nucleotide sequences of hepatitis B virus were revealed in an integrated (chromosomal) and free states by means of dot- and blot-hybridization with a cloned HBV-probe. These observations support the conclusion made earlier on the lack of strict hepatotropism for HBV and, in addition, point to a possible involvement of HBV genomes in some molecular pathologies of men.

Base Sequence↗
PubMed1985

[Regions of the human genome containing homologs of the oncogenes and retrovirus genes. 3. The primary structure of the mos-related region CL-1 from the ORAgp5 locus].

The nucleotide sequence of 686 bp from the cloned human genome locus gp5 has been determined. Analysis of this sequence has revealed a statistically significant homology with both the viral and murine mos genes. The region of mos homology contains two adjacent homologous domains, whereas their counterparts in viral mos gene are separated by 471 bp. The position of mos homologous region in the close vicinity to LTR of endogenous human viral-like repeat is in accordance with the hypothesis of retroviral involvement in the process of mos gene amplification.

Base Sequence↗
PubMed1981

[Dependence of the anticodon stem conformation of tRNA Trp on the anticodon loop structure].

After conversion of the exposed cytosine bases of the anticodon loop into uridines with bisulfite the pattern of hydrolysis of the tRNATrp anticodon stem under the action of cobra venom endonuclease is altered. A removal of the 3'-CCA end has no influence on the hydrolytic pattern of the anticodon stem in tRNATrp. Thus, the nucleotide sequence of the anticodon loop influences the conformation of the neighbouring stem.

Anticodon↗