PubMed Health⌕ Search

Biomedical subjects

L L Olson

Publications and source records attributed to L L Olson.

11 recordsLinked to original sources
PubMed1998

Improving certified nurse aide retention. A long-term care management challenge.

In the long-term care industry, the turnover rate among nurse aides is extremely high. This adversely affects resident satisfaction, resident care, morale, and finances. It presents a challenge to long-term care administration. Refusing to accept high turnover as an impossible situation allows changes to be made. The authors describe how the staff at one intermediate care facility identified its problems, assessed the causes, and implemented corrective action.

Absenteeism↗
PubMed1998

Hospital nurses' perceptions of the ethical climate of their work setting.

PURPOSE: To develop an instrument to measure how hospital nurses perceive the ethical climate of their work setting and to evaluate its psychometric properties. A way to measure ethical climate can help nurses understand the work setting influences on their practice and can be used to diagnose areas for organizational change as well as to evaluate effectiveness of organizational interventions. DESIGN: Descriptive. Instrument developed was the Hospital Ethical Climate Survey (HECS). Sampled were 360 RNs (48% response rate) employed in clinical practice at two acute-care hospitals in one U.S. midwestern city. The nurses completed a questionnaire in 1993-1994 consisting of items developed to assess their perceptions of ethical climate, along with the 43-item Integrity Audit, a social desirability scale, and a demographic inventory. METHODS: Construct validity was assessed by confirmatory factor analysis (CFA) using the LISREL 7 statistical program. RESULTS: The final CFA model consisted of 26 variables in five factors, which were organized according to the relationships of nurses with peers, patients, managers, hospital, and physicians. Internal consistency reliability, using Cronbach's alpha, for the final 26-item instrument was 0.91 (range 0.68 to 0.92) for the subscales. CONCLUSIONS: The Hospital Ethical Climate Survey has acceptable initial reliability and validity.

Attitude of Health Personnel↗
PubMed1990

31P-NMR assay of phosphatidylcholine and phosphatidylethanolamine in AL721.

A 31P-NMR based method has been developed for the assay of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) in AL721. The assay is based on the comparison of NMR signal intensities of the phosphatidyl moiety of PC and PE to the signal of triphenylphosphate (TPP), the internal standard. The assay is specific and reliable for the quantitation of phospholipid mixtures. Its uncertainty, due primarily to low intensity of 31P signals, was estimated at less than 6%.

Drug Combinations↗
PubMed1990

1H-NMR assay of phosphatidylcholine and phosphatidylethanolamine in AL721.

A 1H-NMR based method has been developed for the assay of phosphatidylcholine (PC), and phosphatidylethanolamine (PE) in AL721. The assay was based on the comparison of NMR signal intensities of the choline and the ethanolamine moiety of PC and PE, respectively, to the signal of imidazole, the internal standard. The assay was reliable and more accurate than the commonly used LC or TLC methods. The uncertainty, due primarily to signal intensities, of the 1H-NMR method was estimated at less than 3%.

Chromatography, Liquid↗
PubMed1984

Regulation of excitation energy in a wheat mutant deficient in light-harvesting pigment protein complex.

Chloroplasts of the CD3 wheat mutant were deficient primarily in chlorophyll of light harvesting pigment proteins (LHPP) 1 and 2 and CP1a. The reduced level of protein associated with chlorophyll of LHPP1 and LHPP2 and the reduced level of low molecular weight polypeptides between 23 and 29 kilodaltons confirmed that the CD3 mutant was deficient in the LHPP complex. The high fluorescence emission ratio at 740 (F740) to 686 nanometers (F686) observed from chloroplasts of normal wheat following light induced phosphorylation of the LHPP complex was not noted from mutant chloroplasts. The long wavelength peak fluorescence emission (F740) was shifted to a shorter wavelength peak (F725) and was reduced in intensity compared to that of normal wheat thylakoids. The ratio of variable fluorescence to maximum fluorescence, a measure of PSII photochemical efficiency, was the same for the normal wheat and mutant leaves. The ratios of uncoupled photosystem I/photosystem II electron transport rates for mutant and normal wheat chloroplasts were similar at saturating light suggesting that absorbed excitation energy was distributed to the two photosystem reaction centers of the mutant in a similar manner as in the normal wheat. Proteins of the LHPP complex were differentially phosphorylated by action of a membrane protein kinase when both normal wheat and CD3 mutant thylakoids were irradiated without an electron transport chain acceptor. Even though the F740/F686 ratio was low in mutant thylakoids, the phosphorylation of the 27-kilodalton LHPP polypeptide was consistent with the mutant being in a state II condition. The data gave rise to the suggestion that the F740/F686 ratio might not indicate excitation energy distribution to the two photosystems in the mutant.

Journal Article↗