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Biomedical subjects

L López

Publications and source records attributed to L López.

At least 91 records · Page 5Linked to original sources
PubMed1994

Molecular cloning and nucleotide sequence of the coat protein gene of a Cuban isolate of potato leafroll virus and its expression in Escherichia coli.

Total RNA from infected Physalis floridana was isolated to generate complementary DNA corresponding to the coat protein (GP) gene of a Cuban isolate of potato leaf roll virus (PLRV). This cDNA was amplified by the polymerase chain reaction (PCR) and cloned into the bacterial expression vectors pEX(1-3) for fusion protein expression in E. coli. The product was detected by antibodies specific for the PLRV CP. The coding sequence of the CP gene was determined, and the predicted length of the CP was 208 amino acids (23 kD). The nucleotide sequences and deduced amino acid sequences were compared with the other PLRV isolates and found to be 97-99.5% identical at both the nucleotide and amino acid sequence level of other isolates. Comparison of the deduced amino acid sequences of the PLRVcub CP revealed considerable homology to other luteoviruses. We believe that the protocol described could be applicable to other plant viruses of low abundance or of cumbersome isolation, since this method is less time consuming than the traditional methods of cloning coat protein genes of plant viruses with known sequences.

Amino Acid Sequence↗
PubMed1994

[Development and optimization of an isotonic sports drink].

Within a research program on food products for athletes starting from natural apple concentrate, the design, manufacture and control of isotonic beverages, was undertaken. The dilution conditions for the apple concentrate were established studying several salts combinations which will supply those electrolytes that are eliminated through sweat and have to be replenished. They are sodium, potassium, chloride, magnesium and calcium in concentrations such as to supply an osmotic pressure similar to that observed in blood. The addition of a premix of vitamins (B1, B2, B6, B12, C, E, folic acid, niacin, pantothenic acid and biotin) in several levels of the Recommended Daily Allowance (RDA) (100, 75, 62.5, 60 and 30% of the RDA) was assayed. The obtained formula tasted salty and medicine-like, therefore the vitamin content had to be reduced, supplying 30% of the RDA in a premix constituted by those vitamins which are the most important for physical activity (B1, B2, B6 and C). The quality of the formula was optimized considering variables such as flavor, color and maltodextrin levels by using methods of sensory evaluation. In the elaborated beverage controls such as pH, acidity, total solids (degree Bx), relative density and soluble solids/acidity ratio complying with the corresponding standards, were carried out. In addition, chemical composition, caloric value, microbiological and sensory quality, were controlled. The fruit juice was packed in a 250 ml self-supporting"Doy-Pack"-like bag. Both, microbiological and sensory quality were good, and the caloric value was 101.18 Kcal/250ml. Shelf life studies at two different temperature conditions 15-25 degrees C and 3-5 degrees C, were carried out. The limiting factor was flavor which defined a shelf life of at least 3 months, stored at refrigeration temperature (3-5 degrees C). The formulated beverage supply the electrolyte concentrations which allows to be characterized as isotonic beverage, recommended for athletes.

Beverages↗
PubMed1993

Slow, gradual external fixation distraction for treatment of postburn knee flexion contracture.

Although surgical release and reconstruction have been used extensively to treat severe postburn knee flexion contractures, the degree of correction is sometimes unsatisfactory, and the procedure is not exempt from significant complications. Based on the principle of slow, gradual external fixation distraction, we have designed a triangular articulated frame to be mounted with the ASIF-BM fixator to deal with joint flexion contractures. One successfully treated patient is reported. This is a percutaneous surgery, with very mild aggression to the patient and very low iatrogenic risks. The technique is easy, the degree of correction may be almost complete, and the hospital stay is very short because it is an ambulatory surgery.

Burns↗
PubMed1993

[Development of soy-based protein candy bars for athletes].

Two varieties of soy-based candy bars were developed for sportsmen who need a higher protein intake. The two varieties, almond and nut, were covered with chocolate. The ingredients used were isolated soy protein, texturized soy flour, milk solids, cocoa powder, toasted oat, nuts, almonds, authorized flavors, preservants and antioxidants. Controls were carried out in the optimized products, and the results indicate a very good sensory and microbiological quality. The average nutritional composition of both varieties is: 12,4% proteins, 9% lipids and 58,7% carbohydrates, and the caloric value is 375,2 kcal/100 g. A shelf-life study was performed at room temperature with the candy bars packed in an aluminium foil. Determining that the quality remains without significant changes during 30 days for the nut candy, and at least for 60 days for the almond candy bar.

Animals↗
PubMed1992

Surgical treatment of stage III non-small cell bronchogenic carcinoma involving the chest wall.

Thirty-five patients who had undergone surgery for non-small cell bronchogenic carcinoma with isolated involvement of the chest wall were reviewed. The diagnosis was preoperatively suspected in 80% of cases. En-bloc resection of the invaded chest wall was performed in 25 cases and parietal pleurectomy in ten in which the pleura was easily dissectable from the costal plane. Of the eight patients with major complications in the early postoperative period, six, including the two who died perioperatively, had undergone en-block resection. The 5-year actuarial survival rate was 22% overall and 36% in the patients without lymph node involvement. No significant relationship between survival and type of operation or degree of chest wall invasion was found. Isolated involvement of the chest wall by non-small cell bronchogenic carcinoma does not necessarily contraindicate surgery with curative intent. Parietal pleurectomy is valid in selected cases. Long-term survival depends basically on node involvement.

Actuarial Analysis↗
PubMed1992

[Development of an energy food for athletes].

The energetic requirements of sportsmen fluctuates between 3500 and 8000 Kcal/day. In order to fill these needs it is necessary to have new alternative products which provide the extra required energy. For this purpose a confectionary was designed and elaborated consisting of five different bars, made of natural raw materials (dehydrated fruits, milk, eggs, nuts, fat), which supply an important quantity of fats and carbohtdrates, minerals, and vitamins. The confectioned bars were: papaya, hazelnut, almond, apple and orange. These bars are supposed to be eaten between meals as a complement of the nutrients of the usual diet. The production process is an adaptation of the traditional confectionary techniques and was improved for the 5 bars. The production process of the coating sheet (oblate) was also studied. The controls showed that all the bars had a good microbiological quality and an excellent sensory quality. The humidity fluctuated between 6.85 and 13.66%., with an aw of 0.795. The caloric distribution per cent was 8:40:52. The energetic supply was 520 Kcal/100g; so, the ingest of two displays covers between 25-30% of recommended intake for the most frequently practiced sports. The shelf life studies of the product stored in ambiental conditions (20-25 degrees C and 55-60% RH) showed that the product without additives maintained its good quality up to 60 days. The product plus preservative as well as plus antioxidant stays without quality modifications up to at least 120 days, neither rancid odor no rancid flavor was detected. So, we conclude that it is nor necessary to add any antioxidant. The double sheet polypropilene package film showed very good characteristics in protecting the quality of the product.

Antioxidants↗
PubMed1992

[Functional evaluation of a nutritional energy supplement in athletes].

A nutritional caloric supplement as bar in 5 different flavours was evaluated in 9 sportsmen that ate daily 1040 Kcal/100 g extra during 8 weeks. Anthropometric measurements were used for weight, body fat, lean body mass and functional test for VO2 max, anaerobic threshold (AnT) and anaerobic power (AnP). Both tolerance and acceptability were evaluated too. Other similar sportsmen group was employed as control. Positives answers were registered in VO2 max (8.74%) and AnP (p < or = .04), meanwhile the rest of parameters remained without alterations, the same of control group. Test TAT evidence a high preference and a tolerance without deleterious effect. In conclusion, the product evaluated is an excellent ergogenic aid in effort with elevated energetic demand.

Adult↗
PubMed1988

Lumbar microdiscectomy: analysis of 100 consecutive cases. Its pitfalls and final results.

100 consecutive microsurgically operated patients suffering lumbar prolapsed disc are analyzed in the present work. Three different groups have been distinguished. Under and over forty years of age, and patients operated on after chemonucleolysis failure. General results after all treatments have been 76% good result, 17% improvement and 7% worsening. Special remark should be given about the chemonucleolysis group, that has a highest worsening percentage which raises 25% of the group patients.

Adult↗