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Biomedical subjects

L Lakritz

Publications and source records attributed to L Lakritz.

15 recordsLinked to original sources
PubMed1998

Thiamin, riboflavin, and alpha-tocopherol content of exotic meats and loss due to gamma radiation.

Changes in thiamin, riboflavin, and alpha-tocopherol concentrations due to gamma irradiation were followed in alligator, caiman, bison, and ostrich (exotic) meats. The proximate composition showed that the exotic meats generally had lower fat content than domestic animal meats and that the thiamin content of the reptiles was lower. The changes in the vitamins due to irradiation were similar to those previously observed for domestic species. The results indicate that the loss of vitamins in these species is negligible insofar as the American diet is concerned, and that the concept of "chemiclearance" is applicable to exotic meats.

Analysis of Variance↗
PubMed1993

Effect of reductant level in skeletal muscle and liver on the rate of loss of thiamin due to gamma-radiation.

A study was made of thiamin content of the skeletal muscles and livers of pork, chicken and beef after gamma-irradiation. gamma-Radiation from a 137Cs source was used to irradiate the samples with doses of 0, 1.5, 3, 6 and 10 kGy at 2 degrees C. Samples were also titrated with dichlorophenol-indophenol to determine the reducing capacity of the tissues. The rate of loss of thiamin upon irradiation was found to be about three time as as fast in skeletal muscle as it was in liver, and to be a function of the reducing capacity of the tissues, the loss decreasing with increasing reductant titer. For the same amount of thiamin loss, liver could be irradiated to three times the dose as could muscle.

Animals↗
PubMed1984

Amino acid distribution in brain after use of amphetamines and beta-phenylethylamine.

Amino acids in rat brain were assayed after IP injection d-amphetamine or beta-phenylethylamine (PEA). Results revealed elevated values when one utilized 2.0-12 mg/kg of d-amphetamine. At 15 mg/kg, however, all amino acids fell into the control range except tryptophan which was elevated nearly threefold, and methionine which showed a tenfold decrease. When utilizing PEA to induce the behavioral changes only methionine is decreased at all concentrations of PEA. Chlorpromazine did not disturb the amino acid distribution induced by amphetamine or PEA. When haloperidol was utilized as the neuroleptic to prevent behavioral change there was a significant increase above control of all the amino acids including homocysteine. The implications of this are discussed in the text.

Amino Acids↗
PubMed1984

Survey of human milk for volatile N-nitrosamines and the influence of diet on their formation.

Human milk was analysed for volatile N-nitrosamines and the influence of diet on their possible presence and formation was assessed. 175 samples were obtained from 16 different nursing women. Analysis of samples collected at random, to ascertain baseline levels, indicated that 76.5% of the human milk samples contained less than 0.2 ppb N-nitrosodimethylamine (minimum level of reliable measurement). No other volatile nitrosamines were detected. Expressed milk was collected from volunteers over a 6-hr period after eating meals which included bacon (a source of performed nitrosamines) and at times a vegetable high in nitrate. Eating a meal containing bacon did not result in increased nitrosamine levels in milk. In certain individuals, eating a meal of bacon and a vegetable high in nitrate occasionally resulted in higher levels of N-nitrosodimethylamine in their milk.

Chromatography, Gas↗
PubMed1982

Nitrosamine levels in human blood, urine and gastric aspirate following ingestion of foods containing potential nitrosamine precursors or preformed nitrosamines.

In studies of the effect of diet on nitrosamine levels in selected human physiological fluids, volunteers were fed meals containing fish or beef (sources of precursor amines) or bacon (a source of preformed nitrosamines), in combination with spinach and vegetable juice to supply nitrite via possible reduction of nitrate. Blood, urine and gastric contents were sampled periodically for up to 4 hr after feeding. The results of the study indicated that traces of nitrosamines, usually N-nitrosodimethylamine, were present in many samples of blood, urine and gastric contents, even after an 8-hr fast. Eating the test meals led to a slight increase in nitrosamine levels in the blood and stomach contents in a few subjects. The data obtained from this study suggest that gastric formation of nitrosamine does not appear to be an important health factor in normal people, since the levels of nitrosamines found in physiological fluids are not markedly increased after eating.

Adult↗
PubMed1982

Interaction between sample preparation techniques and three methods of nitrite determination.

Meat samples containing nitrite and varying concentrations of ascorbate, cysteine, and sodium chloride were prepared for nitrite analysis. The methods used were the AOAC method of dilution and heating; the addition of mercuric chloride, charcoal, and Carrez reagents at 2 different pH values; and direct analysis of sample supernatants with no treatment (control). The effect of these initial conditions and preparation methods on measured nitrite was determined by using 3 different Griess reagent combinations and chemiluminescent and differential pulse polarographic techniques. Systematic variations were observed in samples treated with mercuric chloride, while the addition of Carrez reagents had little or no effect. Best results were obtained by the AOAC dilution/heating method under alkaline conditions, or by charcoal addition followed by chemiluminescent or colorimetric nitrite determination. Statistical analysis of the nitrite concentrations determined in the several samples showed that these 3 procedures were precise to about 5-6% CV, which was not significantly different from the CV value of 4% determined from replicate analyses.

Indicators and Reagents↗
PubMed1978

Preliminary observations on amines and nitrosamines in non-normal human gastric contents.

Gastric contents from fasting humans were pooled and analysed for amines. Volatile amines present were dimethylamine, trimethylamine and histamine; non-volatile amines found were cadaverine, putrescine, ethanolamine and tryptamine. Gastric contents from 35 patients, some before and/or after gastric stimulation with histamine or pentagastrin, were analysed for nitrosamines. N-Nitrosodiethylamine (NDEA) (5-30 microgram/kg) was present in four samples, N-nitrosodimethylamine (NDMA) (2 microgram/kg) in two samples and N-nitrosopyrrolidine (NPYR) (6 microgram/kg) in one sample. pH, nitrite and nitrate determinations were made on some samples. Medical diagnosis of patients could not be correlated with the presence of nitrosamines in the gastric contents.

Amines↗