Identity of V factor in culture medium used for prior growth of two strains of Staphylococcus aureus.
Staphylococcus aureus ATCC 12598 and ATCC 25923 were starved of pyridine nucleotides and precursors and then grown in a semidefined medium containing [carbonyl-14C]nicotinamide. Samples of medium from late-exponential-phase and stationary-phase cultures were analyzed for 14C-metabolites. In all cases, V factor was present primarily as NAD.