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Biomedical subjects

L Lorente

Publications and source records attributed to L Lorente.

At least 37 records · Page 2Linked to original sources
PubMed1994

Behaviour of nucleolar organizer regions in the different Wistar rat liver lobes.

The area and number of silver-nucleolar organizer regions (Ag-NORs) in the hepatic lobes were determined in 3 male Wistar rats. There was a statistically significant increase in the percentage of Ag-NORs per nucleus in the right lateral and caudate lobe in relation to the left lateral and middle lobes. The area and number of Ag-NORs are greater in the caudate and right lateral lobes in relation to the left lateral and middle lobes. Since the Ag-NOR is a parameter which indicates hepatocytic protein synthesis, the different activity which corresponds to each lobe of the rat's liver makes it possible to assume that there is a functional heterogeneity which should be considered in the study of the hepatic regeneration according to the type of partial hepatectomy carried out.

Animals↗
PubMed1994

Behavior of nucleolar organizer regions in the different hepatic lobes after end-to-side portacaval shunt in the rat.

Protein synthesis activity in the hepatic lobes in control and end-to-side portacaval shunt rats was studied by assaying one of the argyrophilic components (Ag-NOR) of the hepatocytic nucleolus. The differences found in the area and number of Ag-NOR for each nucleus and in the percentage of Ag-NOR area to nucleus area in each hepatic lobe in control rats as well as in the end-to-side portacaval shunt rats suggest that there are interlobular differences in the control rats related to this parameter that are indicative of protein synthesis and cell proliferation. Furthermore, the changes that occur in these parameters after the deprivation of portal flow produced by an end-to-side portacaval shunt make it possible to hypothesize on the existence of a hepatic lobular functional heterogeneity in the rat liver.

Animals↗
PubMed1994

Hemorrhagic gastroesophageal ulceration by pulmonary infection in extrahepatic cholestatic pigs.

We developed a biliary and pulmonary microbiologic study in 22 Large-White pigs that underwent bile-duct ligation in order to demonstrate that sepsis has a biliary and pulmonary origin which may be involved in the gatroesophageal pathology. All the pigs died at 18.2 +/- 8.9 days of the post-operative period. The cause of death was hemorrhagic ulceration of the gastroesophageal region in 36.3% (n = 8) of the animals that also presented multiple bilateral miliary lung abscesses. High infestation rates with intestinal germs were found in the bile and lung. In conclusion, the experimental model of extrahepatic cholestasis in the Large-White pig could be useful for the study of etiopathogenic mechanisms by which the pulmonary infection produces a hemorrhagic gastroesophageal ulceration considered as stress ulcer.

Animals↗
PubMed1993

A model of cholestasis in the rat, using a microsurgical technique.

An experimental model of extrahepatic cholestasis in the rat, using a microsurgical technique, is described. Sixteen days postoperatively all of the animals (n = 10) were alive and had hepatomegaly, splenomegaly, jaundice, and hyperbilirubinemia. The use of this technique prevents the development of hepatic cysts and other complications inherent in the surgical techniques of cholestasis, such as hepatopneumonic abscesses.

Animals↗
PubMed1991

Comparative study on the antithrombotic efficacy of four low-molecular-weight heparins in three different models of experimental venous thrombosis.

In a randomized, blind study, both the antithrombotic efficacy (reduction of thrombus weight) and potency (anti-Xa activity) of several commercially available low-molecular-weight heparins (LMWHs) were compared with those of unfractioned heparin (UFH) and placebo. Three different thrombogenic challenges were used: venous thrombosis was induced by direct endothelial damage in 60 New Zealand rabbits (group I), intracarotid injection of bovine thrombin in an additional series of 60 rabbits (group II), or after inferior-vena-cava ligature in 60 Wistar rats (group III). The drugs were administered subcutaneously 2 h before surgery in a blind fashion. The doses recommended for clinical practice were used (adjusted by body weight), except in group II animals, in whom doses were doubled. No differences were found between UFH and most LMWHs in terms of reduction of thrombus weight in group I animals. But UFH showed a weaker antithrombotic efficacy in the other two models. Similarly, one of the LMWHs used (Clexane) proved to be not as effective as the remainder. However, only clinical studies will provide enough information to verify these differences. Additionally, our findings confirm that the antithrombotic efficacy of a given drug differs according to the stimulus used to induce venous thrombosis.

Animals↗
PubMed1990

Heterotopic auxiliary liver transplantation with portal flow. Gradual development of the collateral circulation.

One of the causes of auxiliary liver transplantation failure is the inter-liver competition between the host liver and the graft for the hepatotrophic factors of the portal blood. We have developed an experimental model of heterotopic partial (30%) liver isotransplant using Wistar rats so as to study this competition. Splenoportography and dissection demonstrate the existence of collateral circulation. The collaterals at 90 days post-transplant (PT) consisted of veins from the portal vein to the host liver (PR), paraesophageal veins (PE) and splenorenal veins (SR). At 60 days P.T., PR and SR veins but not PE ones appeared, and at 30 days P.T., there were only PR veins. Graft atrophy at 90 days P.T. was associated with a severe degree of bile duct proliferation. The gradual development of portal hypertension causes porto-systemic collateral circulation and the graft loses the portal hepatotrophic factors. The late development of the portal hypertension and the biliary proliferation could be caused by the hepatic arterial ischemia in this experimental model. Thus, as has been described in the orthotopic liver transplantation, the heterotopic one might require a double vascularization, both portal and arterial.

Animals↗
PubMed1990

[Critical Care Surgery Unit in general and digestive system surgery].

In a 5-bed Critical Care Surgery Unit (UQCC) where surgeons are in charge of care, 126 patients admitted over 5 consecutive months were divided into 4 groups according to the treatment given: Group I (n = 49): elective gastrointestinal surgery; Group II (n = 52): emergency gastrointestinal surgery; Group III (n = 15): non-gastrointestinal surgery; Group IV (n = 10): medical treatment. The mean stay of all patients in the Surgical Critical Care Unit was 6 +/- 7.7 days and the global mortality was 7.9% (n = 10). The mean age of the patients who died was 71.5 +/- 7.3 years and 70% of the deaths corresponded to Group II. Forty percent of the patients who died had systemic candidiasis. Among the factors implicated in the mortality acute gastrointestinal pathology per se, the coexistence of chronic systemic disease and advanced age were prominent. We discuss the need for typifying the role of Surgical Critical Care Units in General and Gastrointestinal Surgery.

Adolescent↗
PubMedDate not supplied

[Quality improvement of the filling medication trolleys process, for an unit-dose drug distribution system].

OBJECTIVE: To assess the quality of filling medication trolleys process for an unit-dose drug distribution system, after the implantation of a protocol. METHOD: Five criteria were defined: four were related to the medication given to the patient, and one criterion was related to the patient's identification. At the same time, it was designed a standardized protocol of filling medication trolleys process and it was evaluated the degree of compliance with the criteria on all the hospitalized patients medication drawers in two clinical units. The fulfilment of the criteria was measured both before and after the implantation of the protocol, for fourteen and seven days respectively. RESULTS: In the first evaluation the number of errors was 0.84/medication drawer. After the implantation of the protocol, the total number of errors after correction for sample size decreased at 0.22/medication drawer. The degree of compliance improved for all the criteria, and differences were statistically significant for the criteria with most errors. CONCLUSIONS: The implantation of a protocol significantly improved two of the five quality criteria studied in the filling medication trolleys process. The patient's identification has been revealed as a fundamental aspect of intervention in the dispensation quality.

Clinical Protocols↗