Biomedical subjects

L M Doherty

Publications and source records attributed to L M Doherty.

3 recordsLinked to original sources

PubMed2001

Reference frame for rapid visual processing of line orientation.

Detection of a uniquely oriented line element in a background field of uniformly oriented line elements depends on the orientation of the background field. Is the orientational reference frame for this anisotropy entirely dependent on the orientations of structures outside the line-element display, the spatial regularity of the stimulus elements, and the direction of gravity? The effects of these potential cues were investigated in target-detection experiments with brief displays. The anisotropy was found whether or not gravitational or visual cues defined an orientational reference frame. Stimulus orientation may be coded with respect to the retina or body axis in rapid visual processing.

Anisotropy↗

PubMed1999

Limitations of rapid parallel processing in the detection of long and short oriented line targets.

The purpose of this study was to determine whether the detectability of a uniquely oriented line element in a field of uniformly oriented line elements depends on element length. Displays containing various numbers of elements were presented briefly and followed by a mask. The length and orientation of the elements were varied. With longer (1.0-deg) elements, detection performance varied little with the number of elements present. With shorter (0.25-deg) elements, performance worsened as the element number increased, especially when the uniformly oriented elements were oblique. It seems that rapid spatially parallel processes facilitate detection of targets in many-element displays of long elements but not of short elements.

Adult↗

PubMed1988

Quantitative determination of nuclear estrogen receptors by an enzyme immunoassay: applicability and caveats.

A method is presented with which approx. 95% of nuclear estrogen receptors appear to be extracted from MCF-7 cells. Since both nuclear isolation and nuclear estrogen receptor extraction take place in a single test tube with only vortex mixing, loss of nuclear material is minimized. The amount of nuclear estrogen receptors in the nuclear extract was determined by direct [3H]estradiol labeling of monolayer cultures and with a commercially available estrogen receptor immunoassay (ER-EIA) kit. Since the ER-EIA kit was designed and calibrated for quantitative determination of cytosolic estrogen receptor isolated in low ionic strength buffer, the applicability of the ER-EIA to quantitative determination of estrogen receptor content in high ionic strength nuclear extraction buffer was tested. A linear relationship exists between the amount of nuclear estrogen receptor detected by the immunoassay, the amount of receptor present in serial dilutions of the nuclear extract and the amount of nuclear estrogen receptor detected in cells by [3H]estradiol labeling of monolayer cultures, the absolute amount of nuclear estrogen receptors determined by the immunoassay consistently exceeded the amount of receptor detected by [3H]estradiol labeling. The possibility that the enzyme immunoassay must be properly calibrated for the specific conditions of the nuclear estrogen receptor assay is discussed.

Blotting, Western↗