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L M Firsov

Publications and source records attributed to L M Firsov.

22 records · Page 2Linked to original sources
PubMed1976

[Mechanism of the phosphoglucomutase reaction].

The points of bonds ruptures in the course of phosphoglucomutase reaction were studied by two methods: incorporation of 18O and kinetic isotope effect with the use of double label (14C and 3H). The method of 18O determination by means of activation analysis is described. Analysis of data leads to the conclusion that phosphoryl, but not phosphate group, moves in the course of the reaction. Possible mechanisms of phosphoglutcomutase reaction are discussed.

Catalysis↗
PubMed1975

A study of the mechanism of action of phosphorylase B using isotope-labelled substrates.

We have measured the alpha-deuterium and alpha-tritium secondary kinetic isotope effects on the synthesis and phosphorolysis of glycogen catalyzed by glycogen phosphorylase B (EC 2.4.1.1). The isotope-labeled substrates glucose-1T-1-phosphate and 1C14-glucose-1D-1-phosphate were used in the experiments, together with 6T-glucose-1-phosphate and C14-glucose-1-phosphate as controls. Measurement by the double label technique showed that both effects studied were equal to zero within the limits of experimental error. The possible mechanism of action of phosphorylase B is discussed.

Alkaline Phosphatase↗